Transcript: Human NR_003227.1

Homo sapiens AFG3 like matrix AAA peptidase subunit 1, pseudogene (AFG3L1P), transcript variant 2, non-coding RNA.

Source:
NCBI, updated 2018-05-12
Taxon:
Homo sapiens (human)
Gene:
AFG3L1P (172)
Length:
2947
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_003227.1
NBCI Gene record:
AFG3L1P (172)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_003227.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000006678 CCGGTTTCATCGCCAGATTTA pLKO.1 916 3UTR 100% 13.200 18.480 N AFG3L1P n/a
2 TRCN0000006680 ACAAACAATCTGTGCGTGTTA pLKO.1 649 3UTR 100% 4.950 6.930 N AFG3L1P n/a
3 TRCN0000006677 GCTGGACAAGAGCCTCAATAA pLKO.1 1000 3UTR 100% 13.200 9.240 N AFG3L1P n/a
4 TRCN0000006679 CGGCTGGAAGTCGTGAACAAA pLKO.1 633 3UTR 100% 5.625 3.938 N AFG3L1P n/a
5 TRCN0000006681 AGACCCTTCTTGCCAAAGCAA pLKO.1 730 3UTR 100% 3.000 2.100 N AFG3L1P n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_003227.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10220 pDONR223 100% 11.9% None (many diffs) n/a
2 ccsbBroad304_10220 pLX_304 0% 11.9% V5 (many diffs) n/a
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