Construct: ORF ccsbBroad304_10231
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017971.1_s304c1, BRDN0000409470
- Derived from:
- ccsbBroadEn_10231
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SETDB1 (9869)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10231
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9869 | SETDB1 | SET domain bifurcated histo... | NM_001243491.2 | 100% | 100% | |
| 2 | human | 9869 | SETDB1 | SET domain bifurcated histo... | XM_017002955.1 | 100% | 100% | |
| 3 | human | 9869 | SETDB1 | SET domain bifurcated histo... | NM_012432.4 | 30.5% | 29.7% | (many diffs) |
| 4 | human | 9869 | SETDB1 | SET domain bifurcated histo... | NM_001145415.2 | 30.5% | 29.7% | (many diffs) |
| 5 | human | 9869 | SETDB1 | SET domain bifurcated histo... | NM_001366417.1 | 30.5% | 29.7% | (many diffs) |
| 6 | human | 9869 | SETDB1 | SET domain bifurcated histo... | NM_001366418.1 | 30.5% | 29.7% | (many diffs) |
| 7 | mouse | 84505 | Setdb1 | SET domain, bifurcated 1 | NM_001163642.1 | 27.2% | 27.8% | (many diffs) |
| 8 | mouse | 84505 | Setdb1 | SET domain, bifurcated 1 | NM_001163641.1 | 27.2% | 27.8% | (many diffs) |
| 9 | mouse | 84505 | Setdb1 | SET domain, bifurcated 1 | NM_018877.3 | 27.2% | 27.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1257
- ORF length:
- 1191
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttcccttcct gggtgcattg gtttggatgc agcaacagct acagtggagt 121 ctgaagagat tgcagagctg caacaggcag tggttgagga actgggtatc tctatggagg 181 aacttcggca tttcatcgat gaggaactgg agaagatgga ttgtgtacag caacgcaaga 241 agcagctagc agagttagag acatgggtaa tacagaaaga atctgaggtg gctcacgttg 301 accaactctt tgatgatgca tccagggcag tgactaattg tgagtctttg gtgaaggact 361 tctactccaa gctgggacta caataccggg acagtagctc tgaggacgaa tcttcccggc 421 ctacagaaat aattgagatt cctgatgaag atgatgatgt cctcagtatt gattcaggtg 481 atgctgggag cagaactcca aaagaccaga agctccgtga agctatggct gccttaagaa 541 agtcagctca agatgttcag aagttcatgg atgctgtcaa caagaagagc agttcccagg 601 atctgcataa aggaaccttg agtcagatgt ctggagaact aagcaaagat ggtgacctga 661 tagtcagcat gcgaattctg ggcaagaaga gaactaagac ttggcacaaa ggcaccctta 721 ttgccatcca gacagttggg ccagggaaga aatacaaggt gaaatttgac aacaaaggaa 781 agagtctact gtcggggaac catattgcct atgattacca ccctcctgct gacaagctgt 841 atgtgggcag tcgggtggtc gccaaataca aagatgggaa tcaggtctgg ctctatgctg 901 gcattgtagc tgagacacca aacgtcaaaa acaagctcag gtttctcatt ttctttgatg 961 atggctatgc ttcctatgtc acacagtcgg aactgtatcc catttgccgg ccactgaaaa 1021 agacttggga ggacatagaa gacatctcct gccgtgactt catagaggag tatgtcactg 1081 cctaccccaa ccgccccatg gtactgctca agagtggcca gcttatcaag acTGAGTGGG 1141 AAGGCACGTG GTGGAAGTCC CGAGTTGAGG AGGTGGATGG CAGCCTAGTC AGGATCCTCT 1201 TCCTGGTACT GTTCTTCTCT ACAATTTTGG AGGCAGAGGT TGGTGGGGGG GGAACTTACC 1261 CAACTTTCTT GTACaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1321 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1381 tta