Transcript: Human NM_001243491.2

Homo sapiens SET domain bifurcated histone lysine methyltransferase 1 (SETDB1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
SETDB1 (9869)
Length:
1464
CDS:
113..1306

Additional Resources:

NCBI RefSeq record:
NM_001243491.2
NBCI Gene record:
SETDB1 (9869)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001243491.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000276105 AGTTAGAGACATGGGTAATAC pLKO_005 300 CDS 100% 13.200 18.480 N SETDB1 n/a
2 TRCN0000147130 CAGTGACTAATTGTGAGTCTT pLKO.1 375 CDS 100% 4.950 3.960 N SETDB1 n/a
3 TRCN0000285490 CAGTGACTAATTGTGAGTCTT pLKO_005 375 CDS 100% 4.950 3.960 N SETDB1 n/a
4 TRCN0000305552 TGGCACAAAGGCACCCTTATT pLKO_005 749 CDS 100% 13.200 9.240 N Setdb1 n/a
5 TRCN0000179094 CGTGACTTCATAGAGGAGTAT pLKO.1 1100 CDS 100% 4.950 3.465 N SETDB1 n/a
6 TRCN0000276106 CGTGACTTCATAGAGGAGTAT pLKO_005 1100 CDS 100% 4.950 3.465 N SETDB1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001243491.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10231 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_10231 pLX_304 11.1% 100% 100% V5 n/a
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