Construct: ORF ccsbBroad304_10421
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001237.1_s304c1, BRDN0000400156
- Derived from:
- ccsbBroadEn_10421
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARSA (410)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10421
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 410 | ARSA | arylsulfatase A | NM_000487.6 | 99.5% | 99.4% | 1_6delATGTCC;1178C>G |
| 2 | human | 410 | ARSA | arylsulfatase A | NM_001085425.3 | 99.5% | 99.4% | 1_6delATGTCC;1178C>G |
| 3 | human | 410 | ARSA | arylsulfatase A | NM_001085426.3 | 99.5% | 99.4% | 1_6delATGTCC;1178C>G |
| 4 | human | 410 | ARSA | arylsulfatase A | NM_001085427.3 | 99.5% | 99.4% | 1_6delATGTCC;1178C>G |
| 5 | human | 410 | ARSA | arylsulfatase A | XM_017028800.1 | 92.6% | 92.5% | 1_6delATGTCC;1178C>G;1210_1323del |
| 6 | human | 410 | ARSA | arylsulfatase A | NM_001085428.3 | 83.3% | 83.2% | 0_1ins252;920C>G |
| 7 | human | 410 | ARSA | arylsulfatase A | NM_001362782.2 | 83.3% | 83.2% | 0_1ins252;920C>G |
| 8 | human | 410 | ARSA | arylsulfatase A | XM_011530691.3 | 75.6% | 72.3% | 1_6delATGTCC;1106_1107ins103;1161_1162ins263 |
| 9 | human | 410 | ARSA | arylsulfatase A | XM_024452241.1 | 75.6% | 72.3% | 1_6delATGTCC;1106_1107ins103;1161_1162ins263 |
| 10 | mouse | 11883 | Arsa | arylsulfatase A | NM_009713.4 | 82.9% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1587
- ORF length:
- 1521
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ggcaccgcgg tccctcctcc tggccctggc tgctggcctg gccgttgccc 121 gtccgcccaa catcgtgctg atctttgccg acgacctcgg ctatggggac ctgggctgct 181 atgggcaccc cagctctacc actcccaacc tggaccagct ggcggcggga gggctgcggt 241 tcacagactt ctacgtgcct gtgtctctgt gcacaccctc tagggccgcc ctcctgaccg 301 gccggctccc ggttcggatg ggcatgtacc ctggcgtcct ggtgcccagc tcccgggggg 361 gcctgcccct ggaggaggtg accgtggccg aagtcctggc tgcccgaggc tacctcacag 421 gaatggccgg caagtggcac cttggggtgg ggcctgaggg ggccttcctg cccccccatc 481 agggcttcca tcgatttcta ggcatcccgt actcccacga ccagggcccc tgccagaacc 541 tgacctgctt cccgccggcc actccttgcg acggtggctg tgaccagggc ctggtcccca 601 tcccactgtt ggccaacctg tccgtggagg cgcagccccc ctggctgccc ggactagagg 661 cccgctacat ggctttcgcc catgacctca tggccgacgc ccagcgccag gatcgcccct 721 tcttcctgta ctatgcctct caccacaccc actaccctca gttcagtggg cagagctttg 781 cagagcgttc aggccgcggg ccatttgggg actccctgat ggagctggat gcagctgtgg 841 ggaccctgat gacagccata ggggacctgg ggctgcttga agagacgctg gtcatcttca 901 ctgcagacaa tggacctgag accatgcgta tgtcccgagg cggctgctcc ggtctcttgc 961 ggtgtggaaa gggaacgacc tacgagggcg gtgtccgaga gcctgccttg gccttctggc 1021 caggtcatat cgctcccggc gtgacccacg agctggccag ctccctggac ctgctgccta 1081 ccctggcagc cctggctggg gccccactgc ccaatgtcac cttggatggc tttgacctca 1141 gccccctgct gctgggcaca ggcaagagcc ctcggcagtc tctcttcttc tacccgtcct 1201 acccagacga ggtccgtggg gtttttgctg tgcggagtgg aaagtacaag gctcacttct 1261 tcacccaggg ctctgcccac agtgatacca ctgcagaccc tgcctgccac gcctccagct 1321 ctctgactgc tcatgagccc ccgctgctct atgacctgtc caaggaccct ggtgagaact 1381 acaacctgct ggggggtgtg gccggggcca ccccagaggt gctgcaagcc ctgaaacagc 1441 ttcagctgct caaggcccag ttagacgcag ctgtgacctt cggccccagc caggtggccc 1501 ggggcgagga ccccgccctg cagatctgct gtcatcctgg ctgcaccccc cgcccagctt 1561 gctgccattg cccagatccc catgcctgcc caactttctt gtacaaagtg gttggtaagc 1621 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1681 ggcgcgttaa gtcgacaatc aacctctgga tta