Construct: ORF ccsbBroad304_10447
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003228.1_s304c1, BRDN0000407621
- Derived from:
- ccsbBroadEn_10447
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRAF6 (7189)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10447
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7189 | TRAF6 | TNF receptor associated fac... | NM_004620.4 | 99.8% | 99.6% | 35C>T;1561delG |
| 2 | human | 7189 | TRAF6 | TNF receptor associated fac... | NM_145803.3 | 99.8% | 99.6% | 35C>T;1561delG |
| 3 | human | 7189 | TRAF6 | TNF receptor associated fac... | XM_017018220.1 | 52.6% | 39.5% | (many diffs) |
| 4 | mouse | 22034 | Traf6 | TNF receptor-associated fac... | NM_001303273.1 | 86.1% | 87.5% | (many diffs) |
| 5 | mouse | 22034 | Traf6 | TNF receptor-associated fac... | NM_009424.3 | 86.1% | 87.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1632
- ORF length:
- 1566
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tctgctaaac tgtgaaaaca gctgtggatt cagccagtct gaaagtgact 121 gctgtgtggc catggccagc tcctgtagcg ctgtaacaaa agatgatagt gtgggtggaa 181 ctgccagcac ggggaacctc tccagctcat ttatggagga gatccaggga tatgatgtag 241 agtttgaccc acccctggaa agcaagtatg aatgccccat ctgcttgatg gcattacgag 301 aagcagtgca aacgccatgc ggccataggt tctgcaaagc ctgcatcata aaatcaataa 361 gggatgcagg tcacaaatgt ccagttgaca atgaaatact gctggaaaat caactatttc 421 cagacaattt tgcaaaacgt gagattcttt ctctgatggt gaaatgtcca aatgaaggtt 481 gtttgcacaa gatggaactg agacatcttg aggatcatca agcacattgt gagtttgctc 541 ttatggattg tccccaatgc cagcgtccct tccaaaaatt ccatattaat attcacattc 601 tgaaggattg tccaaggaga caggtttctt gtgacaactg tgctgcatca atggcatttg 661 aagataaaga gatccatgac cagaactgtc ctttggcaaa tgtcatctgt gaatactgca 721 atactatact catcagagaa cagatgccta atcattatga tctagactgc cctacagccc 781 caattccatg cacattcagt acttttggtt gccatgaaaa gatgcagagg aatcacttgg 841 cacgccacct acaagagaac acccagtcac acatgagaat gttggcccag gctgttcata 901 gtttgagcgt tatacccgac tctgggtata tctcagaggt ccggaatttc caggaaacta 961 ttcaccagtt agagggtcgc cttgtaagac aagaccatca aatccgggag ctgactgcta 1021 aaatggaaac tcagagtatg tatgtaagtg agctcaaacg aaccattcga acccttgagg 1081 acaaagttgc tgaaatcgaa gcacagcagt gcaatggaat ttatatttgg aagattggca 1141 actttggaat gcatttgaaa tgtcaagaag aggagaaacc tgttgtgatt catagccctg 1201 gattctacac tggcaaaccc gggtacaaac tgtgcatgcg cttgcacctt cagttaccga 1261 ctgctcagcg ctgtgcaaac tatatatccc tttttgtcca cacaatgcaa ggagaatatg 1321 acagccacct cccttggccc ttccagggta caatacgcct tacaattctt gatcagtctg 1381 aagcacctgt aaggcaaaac cacgaagaga taatggatgc caaaccagag ctgcttgctt 1441 tccagcgacc cacaatccca cggaacccaa aaggttttgg ctatgtaact tttatgcatc 1501 tggaagccct aagacaaaga actttcatta aggatgacac attattagtg cgctgtgagg 1561 tctccacccg ctttgacatg ggtagccttc ggagggaggg ttttcagcca cgaagtactg 1621 atgcagggta tacccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct 1681 cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac 1741 aatcaacctc tggatta