Construct: ORF ccsbBroad304_10450
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014923.1_s304c1, BRDN0000410261
- Derived from:
- ccsbBroadEn_10450
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KEAP1 (9817)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10450
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9817 | KEAP1 | kelch like ECH associated p... | NM_012289.4 | 99.9% | 99.8% | 1870T>A |
| 2 | human | 9817 | KEAP1 | kelch like ECH associated p... | NM_203500.2 | 99.9% | 99.8% | 1870T>A |
| 3 | human | 9817 | KEAP1 | kelch like ECH associated p... | XM_005260173.1 | 99.9% | 99.8% | 1870T>A |
| 4 | human | 9817 | KEAP1 | kelch like ECH associated p... | XM_005260174.1 | 99.9% | 99.8% | 1870T>A |
| 5 | human | 9817 | KEAP1 | kelch like ECH associated p... | XM_011528452.1 | 99.9% | 99.8% | 1870T>A |
| 6 | mouse | 50868 | Keap1 | kelch-like ECH-associated p... | NM_001110305.1 | 86.2% | 93.9% | (many diffs) |
| 7 | mouse | 50868 | Keap1 | kelch-like ECH-associated p... | NM_001110306.1 | 86.2% | 93.9% | (many diffs) |
| 8 | mouse | 50868 | Keap1 | kelch-like ECH-associated p... | NM_001110307.1 | 86.2% | 93.9% | (many diffs) |
| 9 | mouse | 50868 | Keap1 | kelch-like ECH-associated p... | NM_016679.4 | 86.2% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1938
- ORF length:
- 1872
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaagag 61 ttggcatgca gccagatccc aggcctagcg gggctggggc ctgctgccga ttcctgcccc 121 tgcagtcaca gtgccctgag ggggcagggg acgcggtgat gtacgcctcc actgagtgca 181 aggcggaggt gacgccctcc cagcatggca accgcacctt cagctacacc ctggaggatc 241 ataccaagca ggcctttggc atcatgaacg agctgcggct cagccagcag ctgtgtgacg 301 tcacactgca ggtcaagtac caggatgcac cggccgccca gttcatggcc cacaaggtgg 361 tgctggcctc atccagccct gtcttcaagg ccatgttcac caacgggctg cgggagcagg 421 gcatggaggt ggtgtccatt gagggtatcc accccaaggt catggagcgc ctcattgaat 481 tcgcctacac ggcctccatc tccatgggcg agaagtgtgt cctccacgtc atgaacggtg 541 ctgtcatgta ccagatcgac agcgttgtcc gtgcctgcag tgacttcctg gtgcagcagc 601 tggaccccag caatgccatc ggcatcgcca acttcgctga gcagattggc tgtgtggagt 661 tgcaccagcg tgcccgggag tacatctaca tgcattttgg ggaggtggcc aagcaagagg 721 agttcttcaa cctgtcccac tgccaactgg tgaccctcat cagccgggac gacctgaacg 781 tgcgctgcga gtccgaggtc ttccacgcct gcatcaactg ggtcaagtac gactgcgaac 841 agcgacggtt ctacgtccag gcgctgctgc gggccgtgcg ctgccactcg ttgacgccga 901 acttcctgca gatgcagctg cagaagtgcg agatcctgca gtccgactcc cgctgcaagg 961 actacctggt caagatcttc gaggagctca ccctgcacaa gcccacgcag gtgatgccct 1021 gccgggcgcc caaggtgggc cgcctgatct acaccgcggg cggctacttc cgacagtcgc 1081 tcagctacct ggaggcttac aaccccagtg acggcacctg gctccggttg gcggacctgc 1141 aggtgccgcg gagcggcctg gccggctgcg tggtgggcgg gctgttgtac gccgtgggcg 1201 gcaggaacaa ctcgcccgac ggcaacaccg actccagcgc cctggactgt tacaacccca 1261 tgaccaatca gtggtcgccc tgcgccccca tgagcgtgcc ccgtaaccgc atcggggtgg 1321 gggtcatcga tggccacatc tatgccgtcg gcggctccca cggctgcatc caccacaaca 1381 gtgtggagag gtatgagcca gagcgggatg agtggcactt ggtggcccca atgctgacac 1441 gaaggatcgg ggtgggcgtg gctgtcctca atcgtctcct ttatgccgtg gggggctttg 1501 acgggacaaa ccgccttaat tcagctgagt gttactaccc agagaggaac gagtggcgaa 1561 tgatcacagc aatgaacacc atccgaagcg gggcaggcgt ctgcgtcctg cacaactgta 1621 tctatgctgc tgggggctat gatggtcagg accagctgaa cagcgtggag cgctacgatg 1681 tggaaacaga gacgtggact ttcgtagccc ccatgaagca ccggcgaagt gccctgggga 1741 tcactgtcca ccaggggaga atctacgtcc ttggaggcta tgatggtcac acgttcctgg 1801 acagtgtgga gtgttacgac ccagatacag acacctggag cgaggtgacc cgaatgacat 1861 cgggccggag tggggtgggc gtggctgtca ccatggagcc ctgccggaag cagattgacc 1921 agcagaactg taccagttgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 1981 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2041 agtcgacaat caacctctgg atta