Construct: ORF ccsbBroad304_10472
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004149.1_s304c1, BRDN0000407123
- Derived from:
- ccsbBroadEn_10472
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LNX1 (84708)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10472
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84708 | LNX1 | ligand of numb-protein X 1 | NM_032622.2 | 99.7% | 99.6% | 1890_1893delTTTTinsNNNN |
| 2 | human | 84708 | LNX1 | ligand of numb-protein X 1 | NM_001126328.3 | 85.2% | 82.8% | (many diffs) |
| 3 | human | 84708 | LNX1 | ligand of numb-protein X 1 | XM_005265785.5 | 85.2% | 82.8% | (many diffs) |
| 4 | human | 84708 | LNX1 | ligand of numb-protein X 1 | XM_017008776.1 | 85.2% | 82.8% | (many diffs) |
| 5 | human | 84708 | LNX1 | ligand of numb-protein X 1 | XM_024454261.1 | 85.2% | 82.8% | (many diffs) |
| 6 | human | 84708 | LNX1 | ligand of numb-protein X 1 | XM_024454262.1 | 85.2% | 82.8% | (many diffs) |
| 7 | mouse | 16924 | Lnx1 | ligand of numb-protein X 1 | NM_001159579.1 | 84.7% | 88.6% | (many diffs) |
| 8 | mouse | 16924 | Lnx1 | ligand of numb-protein X 1 | NM_010727.4 | 84.7% | 88.6% | (many diffs) |
| 9 | mouse | 16924 | Lnx1 | ligand of numb-protein X 1 | NM_001159580.1 | 83.3% | 87.1% | (many diffs) |
| 10 | mouse | 16924 | Lnx1 | ligand of numb-protein X 1 | NM_001159578.1 | 76.6% | 79.2% | (many diffs) |
| 11 | mouse | 16924 | Lnx1 | ligand of numb-protein X 1 | XM_017320691.1 | 76.6% | 79.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1962
- ORF length:
- 1896
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggcgcttctg ttgctggtct tgccttggct cagtcctgct aactacattg 121 acaatgtggg caacctgcac ttcctgtatt cagaactctg taaaggtgcc tcccactacg 181 gcctgaccaa agataggaag aggcgctcac aagatggctg tccagacggc tgtgcgagcc 241 tcacagccac ggctccctcc ccagaggttt ctgcagctgc caccatctcc ttaatgacag 301 acgagcctgg cctagacaac cctgcctacg tgtcctcggc agaggacggg cagccagcaa 361 tcagcccagt ggactctggc cggagcaacc gaactagggc acggcccttt gagagatcca 421 ctattagaag cagatcattt aaaaaaataa atcgagcttt gagtgttctt cgaaggacaa 481 agagcgggag tgcagttgcc aaccatgccg accagggcag ggaaaattct gaaaacacca 541 ctgcccctga agtctttcca aggttgtacc acctgattcc agatggtgaa attaccagca 601 tcaagatcaa tcgagtagat cccagtgaaa gcctctctat taggctggtg ggaggtagcg 661 aaaccccact ggtccatatc attatccaac acatttatcg tgatggggtg atcgccagag 721 acggccggct actgccagga gacatcattc taaaggtcaa cgggatggac atcagcaatg 781 tccctcacaa ctacgctgtg cgtctcctgc ggcagccctg ccaggtgctg tggctgactg 841 tgatgcgtga acagaagttc cgcagcagga acaatggaca ggccccggat gcctacagac 901 cccgagatga cagctttcat gtgattctca acaaaagtag ccccgaggag cagcttggaa 961 taaaactggt gcgcaaggtg gatgagcctg gggttttcat cttcaatgtg ctggatggcg 1021 gtgtggcata tcgacatggt cagcttgagg agaatgaccg tgtgttagcc atcaatggac 1081 atgatcttcg atatggcagc ccagaaagtg cggctcatct gattcaggcc agtgaaagac 1141 gtgttcacct cgtcgtgtcc cgccaggttc ggcagcggag ccctgacatc tttcaggaag 1201 ccggctggaa cagcaatggc agctggtccc cagggccagg ggagaggagc aacactccca 1261 agcccctcca tcctacaatt acttgtcatg agaaggtggt aaatatccaa aaagaccccg 1321 gtgaatctct cggcatgacc gtcgcagggg gagcatcaca tagagaatgg gatttgccta 1381 tctatgtcat cagtgttgag cccggaggag tcataagcag agatggaaga ataaaaacag 1441 gtgacatttt gttgaatgtg gatggggtcg aactgacaga ggtcagccgg agtgaggcag 1501 tggcattatt gaaaagaaca tcatcctcga tagtactcaa agctttggaa gtcaaagagt 1561 atgagcccca ggaagactgc agcagcccag cagccctgga ctccaaccac aacatggccc 1621 cacccagtga ctggtcccca tcctgggtca tgtggctgga attaccacgg tgcttgtata 1681 actgtaaaga tattgtatta cgaagaaaca cagctggaag tctgggcttc tgcattgtag 1741 gaggttatga agaatacaat ggaaacaaac cttttttcat caaatccatt gttgaaggaa 1801 caccagcata caatgatgga agaattagat gtggtgatat tcttcttgct gtcaatggta 1861 gaagtacatc aggaatgata catgcttgct tggcaagact gctgaaagaa cttaaaggaa 1921 gaattactct aactattgtt tcttggcctg gcacnnnntt atacccaact ttcttgtaca 1981 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2041 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta