Construct: ORF ccsbBroad304_10479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014404.1_s304c1, BRDN0000460992
- Derived from:
- ccsbBroadEn_10479
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM81A (145773)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10479
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145773 | FAM81A | family with sequence simila... | NM_152450.3 | 98.8% | 98.9% | (many diffs) |
| 2 | human | 145773 | FAM81A | family with sequence simila... | XM_006720398.3 | 98.8% | 98.9% | (many diffs) |
| 3 | human | 145773 | FAM81A | family with sequence simila... | XM_011521249.2 | 98.8% | 98.9% | (many diffs) |
| 4 | human | 145773 | FAM81A | family with sequence simila... | XM_011521250.2 | 98.8% | 98.9% | (many diffs) |
| 5 | human | 145773 | FAM81A | family with sequence simila... | XM_024449846.1 | 98.8% | 98.9% | (many diffs) |
| 6 | human | 145773 | FAM81A | family with sequence simila... | XM_005254166.2 | 96.1% | 95.7% | (many diffs) |
| 7 | human | 145773 | FAM81A | family with sequence simila... | XM_011521247.2 | 96.1% | 95.7% | (many diffs) |
| 8 | human | 145773 | FAM81A | family with sequence simila... | XM_011521248.2 | 96.1% | 95.7% | (many diffs) |
| 9 | human | 145773 | FAM81A | family with sequence simila... | XM_017021931.1 | 96.1% | 95.7% | (many diffs) |
| 10 | human | 145773 | FAM81A | family with sequence simila... | XM_017021932.1 | 96.1% | 95.7% | (many diffs) |
| 11 | mouse | 76886 | Fam81a | family with sequence simila... | NM_029784.2 | 87.2% | 92.6% | (many diffs) |
| 12 | mouse | 76886 | Fam81a | family with sequence simila... | XM_006511550.3 | 87.2% | 92.6% | (many diffs) |
| 13 | mouse | 76886 | Fam81a | family with sequence simila... | XM_006511551.2 | 87.2% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1161
- ORF length:
- 1095
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca tctaaggcga gttagaacca tgccccgaca cagccagtcc ctgacaatgg 121 caccatactc atctgtaagc ctcgtggagc agctggaaga caggatcctc tgccatgaga 181 aaaccaccgc cgccctcgta gagcacgcct ttcggattaa agatgacatt gtcaacagtt 241 tgcagaaaat gcaaaacaaa gggggaggtg accgcttggc caggcttttc ttggaggagc 301 atatcagaaa cataactgcc atagtgaagc aacttaatcg ggatatcgag gtactccagg 361 agcagattcg tgctcgggac aacattagct atggaactaa ttctgcctta aagaccctgg 421 agatgcgcca gctctccggt ttgggagatc ttcgaggaag agtggcaaga tgtgatgcca 481 gcatagctag actttctgca gagcacaaaa cgacctatga ggggctccag cacttgaaca 541 aagaacagca ggctgccaaa cttatcttgg aaacgaaaat caaagatgca gagggacaga 601 tttctcagct tttgaacaga gtggacttgt caatatcaga gcagagcacc aaactgaaga 661 tgtctcacag agacagtaac caccagcttc agcttttgga cactaaattt aaaggtacag 721 ttgaggaact cagtaaccag atattatctg cacggagttg gttgcaacag gaacaagaac 781 ggatagaaaa agagctttta cagaaaattg atcagctttc cttgattgtt aaggaaaaca 841 gtggagccag tgaaagggat atggagaaga agctcagcca gatgtcagcc aggcttgaca 901 aaatagaaga gggtcaaaag aagacttttg atggtcagag aacaaggcaa gaagaggaga 961 agatgcacgg gcgaatcacc aagctggagt tacagatgaa ccagaacatc aaggaaatga 1021 aagcagaagt taatgctggg tttacagccg tctatgaaag cataggatcc atcaggcaag 1081 ttctcgaggc caagatgaag ctggacaggg accagctaca gaagcaaatc cagctgatgc 1141 agaagccaga gacccccatg tgcccaactt tcttgtacaa agtggttggt aagcctatcc 1201 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1261 ttaagtcgac aatcaacctc tggatta