Construct: ORF ccsbBroad304_10649
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009523.1_s304c1, BRDN0000401046
- Derived from:
- ccsbBroadEn_10649
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A51P4 (494141)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10649
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92014 | SLC25A51 | solute carrier family 25 me... | NM_033412.4 | 45% | 33% | (many diffs) |
| 2 | human | 147407 | SLC25A52 | solute carrier family 25 me... | NM_001034172.4 | 44.1% | 29.7% | (many diffs) |
| 3 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026564.1 | 26.5% | 1_238del;411C>T;701_1739del | |
| 4 | human | 442229 | SLC25A51P1 | SLC25A51 pseudogene 1 | NR_026540.1 | 26.2% | (many diffs) | |
| 5 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026563.1 | 24.4% | 1_383del;556C>T;846_1884del | |
| 6 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026541.1 | 22% | 1_238del;411C>T;701_2093del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 528
- ORF length:
- 462
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa aaaagaagaa cttaagcaac atgatggatt cagaagctca tggaaagaga 121 ccaccaatac taacatcttc gaaacaagat atgtcacctc atattacaga tttagtgaaa 181 tgaagcatta cttgtgtggc tgctgtgcag ccttcaacaa cgtcgcaatc acatttctca 241 ttcagaaggt cctctttcca caacagctgt atggcatcaa aacgggggat gcaatacttc 301 agttgagaac ggatggattt cgaaacttgt atcggggaat ctttccccga ttgatgcaga 361 agacaactac acttgcactt acgtttggtc tgtatgagga tttatcctac cttctccaca 421 agcatgtcag tgctccagag tttgcaacct gtggcgtggc agcagtgctt gcagggacaa 481 cggaagcaat tttcacttca gacattgctt caagaccaca agcaccatgc ccaactttct 541 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 601 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta