Construct: ORF ccsbBroad304_10699
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015994.1_s304c1, BRDN0000401919
- Derived from:
- ccsbBroadEn_10699
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRCA1 (672)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10699
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NM_007297.4 | 72.3% | 72.1% | (many diffs) |
| 2 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NM_007300.4 | 71.6% | 71.4% | (many diffs) |
| 3 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NM_007294.4 | 70.5% | 70.3% | (many diffs) |
| 4 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NR_027676.2 | 55.2% | (many diffs) | |
| 5 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NM_007298.3 | 47.9% | 39.5% | (many diffs) |
| 6 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NM_007299.4 | 43.7% | 33.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4131
- ORF length:
- 4062
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gataaatcag ggaactaacc aaacggagca gaatggtcaa gtgatgaata 121 ttactaatag tggtcatgag aataaaacaa aaggtgattc tattcagaat gagaaaaatc 181 ctaacccaat agaatcactc gaaaaagaat ctgctttcaa aacgaaagct gaacctataa 241 gcagcagtat aagcaatatg gaactcgaat taaatatcca caattcaaaa gcacctaaaa 301 agaataggct gaggaggaag tcttctacca ggcatattca tgcgcttgaa ctagtagtca 361 gtagaaatct aagcccacct aattgtactg aattgcaaat tgatagttgt tctagcagtg 421 aagagataaa gaaaaaaaag tacaaccaaa tgccagtcag gcacagcaga aacctacaac 481 tcatggaagg taaagaacct gcaactggag ccaagaagag taacaagcca aatgaacaga 541 caagtaaaag acatgacagt gatactttcc cagagctgaa gttaacaaat gcacctggtt 601 cttttactaa gtgtccaaat accagtgaac ttaaagaatt tgtcaatcct agccttccaa 661 gagaagaaaa agaagagaaa ctagaaacag ttaaagtgtc taataatgct gaagacccca 721 aagatctcat gttaagtgga gaaagggttt tgcaaactga aagatctgta gagagtagca 781 gtatttcact ggtacctggt actgattatg gcactcagga aagtatctcg ttactggaag 841 ttagcactct agggaaggca aaaacagaac caaataaatg tgtgagtcag tgtgcagcat 901 ttgaaaaccc caagggacta attcatggtt gttccaaaga taatagaaat gacacagaag 961 gctttaagta tccattggga catgaagtta accacagtcg ggaaacaagc atagaaatgg 1021 aagaaagtga actcgatgct cagtatttgc agaatacatt caaggtttca aagcgccagt 1081 catttgctct gttttcaaat ccaggaaatg cagaagagga atgtgcaaca ttctctgccc 1141 actctgggtc cttaaagaaa caaagtccaa aagtcacttt tgaatgtgaa caaaaggaag 1201 aaaatcaagg aaagaatgag tctaatatca agcctgtaca gacagttaat atcactgcag 1261 gctttcctgt ggttggtcag aaagataagc cagttgataa tgccaaatgt agtatcaaag 1321 gaggctctag gttttgtcta tcatctcagt tcagaggcaa cgaaactgga ctcattactc 1381 caaataaaca tggactttta caaaacccat atcgtatacc accacttttt cccatcaagt 1441 catttgttaa aactaaatgt aagaaaaatc tgctagagga aaactttgag gaacattcaa 1501 tgtcacctga aagagaaatg ggaaatgaga acattccaag tacagtgagc acaattagcc 1561 gtaataacat tagagaaaat gtttttaaag gagccagctc aagcaatatt aatgaagtag 1621 gttccagtac taatgaagtg ggctccagta ttaatgaaat aggttccagt gatgaaaaca 1681 ttcaagcaga actaggtaga aacagagggc caaaattgaa tgctatgctt agattagggg 1741 ttttgcaacc tgaggtctat aaacaaagtc ttcctggaag taattgtaag catcctgaaa 1801 taaaaaagca agaatatgaa gaagtagttc agactgttaa tacagatttc tctccatatc 1861 tgatttcaga taacttagaa cagcctatgg gaagtagtca tgcatctcag gtttgttctg 1921 agacacctga tgacctgtta gatgatggtg aaataaagga agatactagt tttgctgaaa 1981 atgacattaa ggaaagttct gctgttttta gcaaaagcgt ccagagagga gagcttagca 2041 ggagtcctag ccctttcacc catacacatt tggctcaggg ttaccgaaga ggggccaaga 2101 aattagagtc ctcagaagag aacttatcta gtgaggatga agagcttccc tgcttccaac 2161 acttgttatt tggtaaagta aacaatatac cttctcagtc tactaggcat agcaccgttg 2221 ctaccgagtg tctgtctaag aacacagagg agaatttatt atcattgaag aatagcttaa 2281 atgactgcag taaccaggta atattggcaa aggcatctca ggaacatcac cttagtgagg 2341 aaacaaaatg ttctgctagc ttgttttctt cacagtgcag tgaattggaa gacttgactg 2401 caaatacaaa cacccaggat cctttcttga ttggttcttc caaacaaatg aggcatcagt 2461 ctgaaagcca gggagttggt ctgagtgaca aggaattggt ttcagatgat gaagaaagag 2521 gaacgggctt ggaagaaaat aatcaagaag agcaaagcat ggattcaaac ttaggtgaag 2581 cagcatctgg gtgtgagagt gaaacaagcg tctctgaaga ctgctcaggg ctatcctctc 2641 agagtgacat tttaaccact cagcagaggg ataccatgca acataacctg ataaagctcc 2701 agcaggaaat ggctgaacta gaagctgtgt tagaacagca tgggagccag ccttctaaca 2761 gctacccttc catcataagt gactcctctg cccttgagga cctgcgaaat ccagaacaaa 2821 gcacatcaga aaaagattcg catatacatg gccaaaggga caactccatg ttttctaaaa 2881 ggcctagaga acatatatca gtattaactt cacagaaaag tagtgaatac cctataagcc 2941 agaatccaga aggcctttct gctgacaagt ttgaggtgtc tgcagatagt tctaccagta 3001 aaaataaaga accaggagtg gaaaggtcat ccccttctaa atgcccatca ttagatgata 3061 ggtggtacat gcacagttgc tctgggagtc ttcagaatag aaactaccca tctcaagagg 3121 ggctcattaa ggttgttgat gtggaggagc aacagctgga agagtctggg ccacacgatt 3181 tgacggaaac atcttacttg ccaaggcaag atctagaggg aaccccttac ctggaatctg 3241 gaatcagcct cttctctgat gaccctgaat ctgatccttc tgaagacaga gccccagagt 3301 cagctcgtgt tggcaacata ccatcttcaa cctctgcatt gaaagttccc caattgaaag 3361 ttgcagaatc tgcccagggt ccagctgctg ctcatactac tgatactgct gggtataatg 3421 caatggaaga aagtgtgagc agggagaagc cagaattgac agcttcaaca gaaagggtca 3481 acaaaagaat gtccatagtg gtgtctggcc tgaccccaga agaatttatg ctcgtgtaca 3541 agtttgccag aaaacaccac atcactttaa ctaatctaat tactgaagag actactcatg 3601 ttgttatgaa aacagatgct gagtttgtgt gtgaacggac actgaaatat tttctaggaa 3661 ttgcgggagg aaaatgggta gttagctatt tctgggtgac ccagtctatt aaagaaagaa 3721 aaatgctgaa tgagcatgat tttgaagtca gaggagatgt ggtcaatgga agaaaccacc 3781 aaggtccaaa gcgagcaaga gaatcccagg acagaaagat cttcaggggg ctagaaatct 3841 gttgctatgg gcccttcacc aacatgccca cagatcaact ggaatggatg gtacagctgt 3901 gtggtgcttc tgtggtgaag gagctttcat cattcaccct tggcacaggt gtccacccaa 3961 ttgtggttgt gcagccagat gcctggacag aggacaatgg cttccatgca attgggcaga 4021 tgtgtgaggc acctgtggtg acccgagagt gggtgttgga cagtgtagca ctctaccagt 4081 gccaggagct ggacacctac ctgatacccc agatccccca cagccactac ttgccaactt 4141 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 4201 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta