Construct: ORF ccsbBroad304_10711
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007666.1_s304c1, BRDN0000398748
- Derived from:
- ccsbBroadEn_10711
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KYAT1 (883)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10711
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 105371063 | LOC105371063 | putative uncharacterized pr... | XM_011522781.1 | 27.9% | 6.7% | (many diffs) |
| 2 | human | 883 | KYAT1 | kynurenine aminotransferase 1 | NR_148224.1 | 12.8% | 1_1691del;1719G>T;2028_2613del | |
| 3 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020991.1 | 8.2% | 6.9% | (many diffs) |
| 4 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020992.1 | 8.2% | 6.9% | (many diffs) |
| 5 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020993.1 | 8.2% | 6.9% | (many diffs) |
| 6 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020994.1 | 8.2% | 6.9% | (many diffs) |
| 7 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020995.1 | 8.2% | 6.9% | (many diffs) |
| 8 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020996.1 | 8.2% | 6.9% | (many diffs) |
| 9 | human | 145282 | MIPOL1 | mirror-image polydactyly 1 | XM_017020997.1 | 8.2% | 6.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 402
- ORF length:
- 336
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg ccaccacacc cggccaattt ttttattttt ggtagagacg gggtttagcc 121 atgttggcca ggctggtctt gaactcctga cctcaggtga tccacccgtc tcagcctccc 181 aaagtgctgg gattataggc gtgagccacc gcacctggcc ttgttcctct gactttgatg 241 cagctctagg tgctggattg gagctaactc tccttccccg cctggtcgtg ggaacctctc 301 tgtcccctcc ggagccaagg cctgctgggc cacgaggccg tgttgagaca tgtccttcct 361 cctccccagg gcttggtggc catccctgtc tccatcttct atacccaact ttcttgtaca 421 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 481 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta