Construct: ORF ccsbBroad304_10718
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003921.1_s304c1, BRDN0000403208
- Derived from:
- ccsbBroadEn_10718
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENTPD3 (956)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10718
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 956 | ENTPD3 | ectonucleoside triphosphate... | NM_001291961.2 | 99.9% | 99.7% | 70A>G |
| 2 | human | 956 | ENTPD3 | ectonucleoside triphosphate... | NM_001248.4 | 85.3% | 85% | 70A>G;1354_1358delGTGGG;1362_1587del |
| 3 | human | 956 | ENTPD3 | ectonucleoside triphosphate... | NM_001291960.2 | 85.3% | 85% | 70A>G;1354_1358delGTGGG;1362_1587del |
| 4 | human | 956 | ENTPD3 | ectonucleoside triphosphate... | XM_011534266.3 | 61.7% | 61.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1422
- ORF length:
- 1356
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt cactgtgctg acccgccaac catgtgagca agcaggcctc aaggccctct 121 accgaactcc aaccgtcatt gccttggtgg tcttgcttgt gagtattgtg gtacttgtga 181 gtatcactgt catccagatc cacaagcaag aggtcctccc tccaggactg aagtatggta 241 ttgtgctgga tgccgggtct tcaagaacca cagtctacgt gtatcaatgg ccagcagaaa 301 aagagaataa taccggagtg gtcagtcaaa ccttcaaatg tagtgtgaaa ggctctggaa 361 tctccagcta tggaaataac ccccaagatg tccccagagc ctttgaggag tgtatgcaaa 421 aagtcaaggg gcaggttcca tcccacctcc acggatccac ccccattcac ctgggagcca 481 cggctgggat gcgcttgctg aggttgcaaa atgaaacagc agctaatgaa gtccttgaaa 541 gcatccaaag ctacttcaag tcccagccct ttgactttag gggtgctcaa atcatttctg 601 ggcaagaaga aggggtatat ggatggatta cagccaacta tttaatggga aatttcctgg 661 agaagaacct gtggcacatg tgggtgcacc cgcatggagt ggaaaccacg ggtgccctgg 721 acttaggtgg tgcctccacc caaatatcct tcgtggcagg agagaagatg gatctgaaca 781 ccagcgacat catgcaggtg tccctgtatg gctacgtata cacgctctac acacacagct 841 tccagtgcta tggccggaat gaggctgaga agaagtttct ggcaatgctc ctgcagaatt 901 ctcctaccaa aaaccatctc accaatccct gttaccctcg ggattatagc atcagcttca 961 ccatgggcca tgtatttgat agcctgtgca ctgtggacca gaggccagaa agttataacc 1021 ccaatgatgt catcactttt gaaggaactg gggacccatc tctgtgtaag gagaaggtgg 1081 cttccatatt tgacttcaaa gcttgccatg atcaagaaac ctgttctttt gatggggttt 1141 atcagccaaa gattaaaggg ccatttgtgg cttttgcagg attctactac acagccagtg 1201 ctttaaatct ttcaggtagc ttttccctgg acaccttcaa ctccagcacc tggaatttct 1261 gctcacagaa ttggagtcag ctcccactgc tgctccccaa atttgatgag gtatatgccc 1321 gctcttactg cttctcagcc aactacatct accacttgtt tgtgaacggt tacaaattca 1381 cagaggagac ttggccccaa atacactttg aaaaagaaga atacccaact ttcttgtaca 1441 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1501 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta