Construct: ORF ccsbBroad304_10832
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016893.1_s304c1, BRDN0000403069
- Derived from:
- ccsbBroadEn_10832
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABRA2 (2555)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10832
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | NM_000807.4 | 79.7% | 79.1% | (many diffs) |
| 2 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | NM_001114175.2 | 79.7% | 79.1% | (many diffs) |
| 3 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_017007982.2 | 79.7% | 79.1% | (many diffs) |
| 4 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_017007983.2 | 79.7% | 79.1% | (many diffs) |
| 5 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453968.1 | 79.7% | 79.1% | (many diffs) |
| 6 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453969.1 | 79.7% | 79.1% | (many diffs) |
| 7 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453970.1 | 79.7% | 79.1% | (many diffs) |
| 8 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453971.1 | 79.7% | 79.1% | (many diffs) |
| 9 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | NM_001330690.1 | 71.2% | 69.4% | (many diffs) |
| 10 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_011513675.1 | 71.2% | 69.4% | (many diffs) |
| 11 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453963.1 | 71.2% | 69.4% | (many diffs) |
| 12 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453964.1 | 71.2% | 69.4% | (many diffs) |
| 13 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453965.1 | 71.2% | 69.4% | (many diffs) |
| 14 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453966.1 | 71.2% | 69.4% | (many diffs) |
| 15 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453967.1 | 71.2% | 69.4% | (many diffs) |
| 16 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453972.1 | 67.6% | 61.8% | (many diffs) |
| 17 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453973.1 | 67.6% | 61.8% | (many diffs) |
| 18 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | XM_024453974.1 | 67.6% | 61.8% | (many diffs) |
| 19 | human | 2555 | GABRA2 | gamma-aminobutyric acid typ... | NM_001286827.3 | 60.4% | 54.2% | (many diffs) |
| 20 | mouse | 14395 | Gabra2 | gamma-aminobutyric acid (GA... | NM_008066.3 | 72.8% | 77.1% | (many diffs) |
| 21 | mouse | 14395 | Gabra2 | gamma-aminobutyric acid (GA... | XM_006503733.3 | 72.8% | 77.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1167
- ORF length:
- 1101
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gacaaaattg aacatctaca acatgcagtt cctgcttttt gttttcttgg 121 tgtgggaccc tgccaggttg gtgctggcta acatccaaga agatgaggct aaaaataaca 181 ttaccatctt tacgagaatt cttgacagac ttctggatgg ttacgataat cggcttagac 241 caggactggg agacagtatt actgaagtct tcactaacat ctacgtgacc agttttggcc 301 ctgtctcaga tacagatatg gaatatacaa ttgatgtttt ctttcgacaa aaatggaaag 361 atgaacgttt aaaatttaaa ggtcctatga atatccttcg actaaacaat ttaatggcta 421 gcaaaatctg gactccagat accttttttc acaatgggaa aaaatcagca gctcataata 481 tgacaatgcc aaataagttg cttcgaattc aggatgatgg gactctgctg tataccatga 541 ggcttacagt tcaagctgaa tgcccaatgc acttggagga tttcccaatg gatgctcatt 601 catgtcctct gaaatttggc agctatgcat atacaacttc agaggtcact tatatttgga 661 cttacaatgc atctgattca gtacaggttg ctcctgatgg ctctaggtta aatcaatatg 721 acctgctggg ccaatcaatc ggaaaggaga caattaaatc cagtacaggt gaatatactg 781 taatgacagc tcatttccac ctgaaaagaa aaattgggta ttttgtgatt caaacctatc 841 tgccttgcat catgactgtc attctctccc aagtttcatt ctggcttaac agagaatctg 901 tgcctgcaag aactgtgttt ggagtaacaa ctgtcctaac aatgacaact ctaagcatca 961 gtgctcggaa ttctctcccc aaagtggctt atgcaactgc catggactgg tttattgctg 1021 tttgttatgc atttgtgttc tctgccctaa ttgaatttgc aactgttaat tacttcacca 1081 aaagaggatg ggcttgggat gggaagagtg tagtaaatga caaggtaagt gagagcttcc 1141 gtgttatcat tattaagaaa ataccctacc caactttctt gtacaaagtg gttggtaagc 1201 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1261 ggcgcgttaa gtcgacaatc aacctctgga tta