Construct: ORF ccsbBroad304_10871

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008230.1_s304c1, BRDN0000404183
Derived from:
ccsbBroadEn_10871
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HELLS (3070)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10871
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3070 HELLS helicase, lymphoid specific NM_001289075.2 80.5% 80.5% 1_252del
2 human 3070 HELLS helicase, lymphoid specific NM_001289074.2 72.9% 72.9% 1_387del
3 human 3070 HELLS helicase, lymphoid specific XM_024447967.1 56.4% 56.4% 1_804del
4 human 3070 HELLS helicase, lymphoid specific NM_001289073.2 49.7% 49.7% 1_1056del
5 human 3070 HELLS helicase, lymphoid specific NM_001289072.2 49.1% 49.1% 1_1080del
6 human 3070 HELLS helicase, lymphoid specific NM_001289071.2 48.7% 48.7% 1_1098del
7 human 3070 HELLS helicase, lymphoid specific NM_001289070.1 47% 47% 1_1176del
8 human 3070 HELLS helicase, lymphoid specific NM_001289069.2 43.1% 43.1% 1_1374del
9 human 3070 HELLS helicase, lymphoid specific NM_001289068.2 42.3% 42.3% 1_1422del
10 human 3070 HELLS helicase, lymphoid specific NM_018063.5 41.5% 41.5% 1_1470del
11 human 3070 HELLS helicase, lymphoid specific NM_001289067.1 39.3% 39.3% 1_1608del
12 human 3070 HELLS helicase, lymphoid specific XM_024447968.1 39.3% 39.3% 1_1608del
13 mouse 15201 Hells helicase, lymphoid specific XM_006526700.3 65.2% 66.6% (many diffs)
14 mouse 15201 Hells helicase, lymphoid specific NM_008234.3 37.9% 38.7% (many diffs)
15 mouse 15201 Hells helicase, lymphoid specific XM_006526699.3 37.9% 38.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1110
ORF length:
1044
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt tggatccagt gagaaagaaa caattgagtt aagtcctact ggtcgaccaa
121aacgacgaac tagaaaatca ataaattaca gcaaaataga tgatttccct aatgaattgg
181aaaaactgat cagtcaaata cagccagagg tggaccgaga aagagctgtt gtggaagtga
241atatccctgt agaatctgaa gttaatctga agctgcagaa tataatgatg ctacttcgta
301aatgttgtaa tcatccatat ttgattgaat atcctataga ccctgttaca caagaattta
361agatcgatga agaattggta acaaattctg ggaagttctt gattttggat cgaatgctgc
421cagaactaaa aaaaagaggt cacaaggtgc tgcttttttc acaaatgaca agcatgttgg
481acattttgat ggattactgc catctcagag atttcaactt cagcaggctt gatgggtcca
541tgtcttactc agagagagaa aaaaacatgc acagcttcaa cacggatcca gaggtgttta
601tcttcttagt gagtacacga gctggtggcc tgggcattaa tctgactgca gcagatacag
661ttatcattta tgatagtgat tggaaccccc agtcggatct tcaggcccag gatagatgtc
721atagaattgg tcagacaaag ccagttgttg tttatcgcct tgttacagca aatactatcg
781atcagaaaat tgtggaaaga gcagctgcta aaaggaaact ggaaaagttg atcatccata
841aaaatcattt caaaggtggt cagtctggat taaatctgtc taagaatttc ttagatccta
901aggaattaat ggaattatta aaatctagag attatgaaag ggaaataaaa ggatcaagag
961agaaggtcat tagtgataaa gatctagagt tgttgttaga tcgaagtgat cttattgatc
1021aaatgaatgc ttcaggacca attaaagaga agatggggat attcaagata ttagaaaatt
1081ctgaagattc cagtcctgaa tgtttgtttt acccaacttt cttgtacaaa gtggttggta
1141agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1201ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)

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