Construct: ORF ccsbBroad304_10885
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006191.1_s304c1, BRDN0000460398
- Derived from:
- ccsbBroadEn_10885
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HLA-L (3139)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10885
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3135 | HLA-G | major histocompatibility co... | XM_017010817.1 | 38.8% | 32.1% | (many diffs) |
| 2 | human | 3135 | HLA-G | major histocompatibility co... | XM_017010818.1 | 38.8% | 32.1% | (many diffs) |
| 3 | human | 3134 | HLA-F | major histocompatibility co... | NM_001098478.2 | 35.9% | 29% | (many diffs) |
| 4 | human | 3133 | HLA-E | major histocompatibility co... | XM_017010807.1 | 35% | 24.3% | (many diffs) |
| 5 | human | 3133 | HLA-E | major histocompatibility co... | XM_017010808.1 | 35% | 24.3% | (many diffs) |
| 6 | human | 3135 | HLA-G | major histocompatibility co... | NM_002127.5 | 30.8% | 24.7% | (many diffs) |
| 7 | human | 3135 | HLA-G | major histocompatibility co... | XM_024446420.1 | 30.8% | 24.7% | (many diffs) |
| 8 | human | 3135 | HLA-G | major histocompatibility co... | NM_001363567.1 | 30.4% | 24.4% | (many diffs) |
| 9 | human | 3105 | HLA-A | major histocompatibility co... | NM_002116.8 | 28.1% | 23% | (many diffs) |
| 10 | human | 3106 | HLA-B | major histocompatibility co... | NM_005514.8 | 27.9% | 21.6% | (many diffs) |
| 11 | human | 3107 | HLA-C | major histocompatibility co... | NM_001243042.1 | 27.9% | 22.5% | (many diffs) |
| 12 | human | 3105 | HLA-A | major histocompatibility co... | NM_001242758.1 | 27.9% | 22.7% | (many diffs) |
| 13 | human | 3107 | HLA-C | major histocompatibility co... | NM_002117.6 | 27.9% | 22.3% | (many diffs) |
| 14 | human | 3134 | HLA-F | major histocompatibility co... | XM_011514564.1 | 27.6% | 22.4% | (many diffs) |
| 15 | human | 3134 | HLA-F | major histocompatibility co... | NM_018950.2 | 27.4% | 22.2% | (many diffs) |
| 16 | human | 3133 | HLA-E | major histocompatibility co... | NM_005516.6 | 27.1% | 21.7% | (many diffs) |
| 17 | human | 3133 | HLA-E | major histocompatibility co... | XM_017010809.2 | 27% | 21.6% | (many diffs) |
| 18 | human | 3134 | HLA-F | major histocompatibility co... | XM_017010813.1 | 24.7% | 20% | (many diffs) |
| 19 | human | 3134 | HLA-F | major histocompatibility co... | XM_017010812.1 | 23% | 18.7% | (many diffs) |
| 20 | human | 3134 | HLA-F | major histocompatibility co... | XM_017010811.1 | 22.5% | 18.2% | (many diffs) |
| 21 | human | 3134 | HLA-F | major histocompatibility co... | XM_017010810.1 | 22.3% | 18.1% | (many diffs) |
| 22 | human | 3134 | HLA-F | major histocompatibility co... | NM_001098479.2 | 22% | 17.8% | (many diffs) |
| 23 | human | 3137 | HLA-J | major histocompatibility co... | NR_024240.1 | 19.7% | (many diffs) | |
| 24 | human | 3134 | HLA-F | major histocompatibility co... | XR_001743373.1 | 9.7% | (many diffs) | |
| 25 | human | 3134 | HLA-F | major histocompatibility co... | XR_001743376.1 | 9.5% | (many diffs) | |
| 26 | human | 3134 | HLA-F | major histocompatibility co... | XR_001743374.1 | 9.4% | (many diffs) | |
| 27 | human | 3139 | HLA-L | major histocompatibility co... | NR_027822.1 | 5.6% | 1_63del;135_136ins229;336_4622del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 570
- ORF length:
- 501
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggtcatg gctccccgaa ccctcctcct gctgctcttg ggggccctgg 121 ccctgaccga gacctgggcc ggtgagtgcg gggtcgggag ggaaagggcc tctgcgggga 181 gaagcgagtg gcccgcccgg cccggggagc cgcgccggga ggagggtcgg gcgggtctca 241 gcctctcctc gcctccaggc tcccactcct tgaggtattt cagcaccgca gtgtcccagc 301 ccggccgcgg ggagccccgg ttcatcgccg tgggctacgt ggacgacaca gagttcgtgc 361 ggttcgacag cgactccgtg agtccgagga tggagcggcg ggcgccgtgg gtggagcagg 421 aggggctgga gtattgggac caggagacac ggaacgccaa gggccacgcg cagatttacc 481 gagtgaacct gcggaccctg ctccgctatt acaaccagag cgaggccggt atgaacagtt 541 cgcctacgat ggcaaggatt acatcgccct tgccaacttt cttgtacaaa gtggttggta 601 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 661 ggtggcgcgt taagtcgaca atcaacctct ggatta