Construct: ORF ccsbBroad304_10949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013099.1_s304c1, BRDN0000410651
- Derived from:
- ccsbBroadEn_10949
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMX1A (4009)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10949
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4009 | LMX1A | LIM homeobox transcription ... | XM_011509538.3 | 33% | 33.1% | 1_606del;810T>C |
| 2 | human | 4009 | LMX1A | LIM homeobox transcription ... | NM_001174069.1 | 26% | 26.1% | 1_846del;1050T>C |
| 3 | human | 4009 | LMX1A | LIM homeobox transcription ... | NM_177398.4 | 26% | 26.1% | 1_846del;1050T>C |
| 4 | mouse | 110648 | Lmx1a | LIM homeobox transcription ... | NM_033652.5 | 23.6% | 25.6% | (many diffs) |
| 5 | mouse | 110648 | Lmx1a | LIM homeobox transcription ... | XM_006496611.2 | 23.6% | 25.6% | (many diffs) |
| 6 | mouse | 110648 | Lmx1a | LIM homeobox transcription ... | XM_017321684.1 | 23.6% | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 366
- ORF length:
- 300
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga aggaatcatg aacccctaca cggctctgcc caccccacag cagctcctgg 121 ccatcgagca gagtgtctac agctcagatc ccttccgaca gggtctcacc ccaccccaga 181 tgcctggaga ccacatgcac ccttatggtg ccgagcccct tttccatgac ctggatagcg 241 acgacacctc cctcagtaac ctgggtgact gtttcctagc aacctcagaa gctgggcctc 301 tgcagtccag agtgggaaac cccattgacc atctgtactc catgcagaat tcttacttca 361 catcttgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 421 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 481 acctctggat ta