Construct: ORF ccsbBroad304_10951
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010287.1_s304c1, BRDN0000412155
- Derived from:
- ccsbBroadEn_10951
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMX1B (4010)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10951
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4010 | LMX1B | LIM homeobox transcription ... | NM_002316.4 | 94.1% | 94.1% | 1_69del |
| 2 | human | 4010 | LMX1B | LIM homeobox transcription ... | NM_001174147.2 | 92.5% | 92.5% | 1_69del;1030_1050del |
| 3 | human | 4010 | LMX1B | LIM homeobox transcription ... | NM_001174146.2 | 91.6% | 91.6% | 1_69del;877_909del |
| 4 | mouse | 16917 | Lmx1b | LIM homeobox transcription ... | NM_010725.2 | 93.3% | 100% | (many diffs) |
| 5 | mouse | 16917 | Lmx1b | LIM homeobox transcription ... | XM_006497746.3 | 85.1% | 91.1% | (many diffs) |
| 6 | mouse | 16917 | Lmx1b | LIM homeobox transcription ... | XM_006497747.3 | 66.9% | 73% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1185
- ORF length:
- 1116
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gttggacggc atcaagatgg aggagcacgc cctgcgcccc gggcccgcca 121 ctctgggggt gctgctgggc tccgactgcc cgcatcccgc cgtctgcgag ggctgccagc 181 ggcccatctc cgaccgcttc ctgatgcgag tcaacgagtc gtcctggcac gaggagtgtt 241 tgcagtgcgc ggcgtgtcag caagccctca ccaccagctg ctacttccgg gatcggaaac 301 tgtactgcaa acaagactac caacagctct tcgcggccaa gtgcagcggc tgcatggaga 361 agatcgcccc caccgagttc gtgatgcggg cgctggagtg cgtgtaccac ctgggctgct 421 tctgctgctg cgtgtgtgaa cggcagctac gcaagggcga cgaattcgtg ctcaaggagg 481 gccagctgct gtgcaagggt gactacgaga aggagaagga cctgctcagc tccgtgagcc 541 ccgacgagtc cgactccgtg aagagcgagg atgaagatgg ggacatgaag ccggccaagg 601 ggcagggcag tcagagcaag ggcagcgggg atgacgggaa ggacccgcgg aggcccaagc 661 gaccccggac catcctcacc acgcagcagc gaagagcctt caaggcctcc ttcgaggtct 721 cgtcgaagcc ttgccgaaag gtccgagaga cactggcagc tgagacgggc ctcagtgtgc 781 gcgtggtcca ggtctggttt cagaaccaaa gagcaaagat gaagaagctg gcgcggcggc 841 accagcagca gcaggagcag cagaactccc agcggctggg ccaggaggtc ctgtccagcc 901 gcatggaggg catgatggct tcctacacgc cgctggcccc accacagcag cagatcgtgg 961 ccatggaaca gagcccctac ggcagcagcg accccttcca gcagggcctc acgccgcccc 1021 aaatgccagg gaacgactcc atcttccatg acatcgacag cgatacctcc ttaaccagcc 1081 tcagcgactg cttcctcggc tcctcagacg tgggctccct gcaggcccgc gtggggaacc 1141 ccatcgaccg gctctactcc atgcagagtt cctacttcgc ctccttgcca actttcttgt 1201 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1261 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a