Construct: ORF ccsbBroad304_10958
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013103.1_s304c1, BRDN0000399234
- Derived from:
- ccsbBroadEn_10958
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP4 (4134)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10958
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4134 | MAP4 | microtubule associated prot... | NM_002375.5 | 84.9% | 84.9% | 1665_2183del |
| 2 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006402.1 | 84.8% | 84.8% | 1665_2183del;3451_3452insGC;3453_3454insC |
| 3 | human | 4134 | MAP4 | microtubule associated prot... | XM_024453529.1 | 83.7% | 83.7% | 223_273del;1716_2234del |
| 4 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006400.2 | 83.6% | 83.5% | (many diffs) |
| 5 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006405.1 | 83.5% | 83.5% | (many diffs) |
| 6 | human | 4134 | MAP4 | microtubule associated prot... | XM_024453530.1 | 82.9% | 82.9% | 221_222ins69;1596_2114del |
| 7 | human | 4134 | MAP4 | microtubule associated prot... | XM_011533719.2 | 82.8% | 82.8% | 223_273del;343_378del;1752_2270del |
| 8 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006395.1 | 82.8% | 82.7% | (many diffs) |
| 9 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006409.2 | 81.6% | 81.6% | 1665_2183del;2859_2860ins114 |
| 10 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006396.2 | 81.6% | 81.6% | (many diffs) |
| 11 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006397.1 | 81.5% | 81.4% | (many diffs) |
| 12 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006411.1 | 81.4% | 81.4% | 528_529ins123;1542_2060del |
| 13 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006412.1 | 81.3% | 81.2% | (many diffs) |
| 14 | human | 4134 | MAP4 | microtubule associated prot... | NM_001134364.2 | 80.9% | 78.7% | (many diffs) |
| 15 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006406.2 | 80.4% | 80.4% | 223_273del;1716_2234del;2910_2911ins114 |
| 16 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006401.1 | 80.2% | 80.2% | 223_273del;579_580ins123;1593_2111del |
| 17 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006413.1 | 78.9% | 78.9% | 1665_2183del;2859_2860ins207 |
| 18 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006410.2 | 78.9% | 78.9% | (many diffs) |
| 19 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006414.1 | 78.9% | 78.8% | (many diffs) |
| 20 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006415.1 | 77.6% | 77.6% | 1665_2183del;2814_2815ins252 |
| 21 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006416.1 | 75.4% | 75.4% | 528_529ins123;1542_2060del;2736_2737ins207 |
| 22 | human | 4134 | MAP4 | microtubule associated prot... | XM_024453527.1 | 43.3% | 42.4% | (many diffs) |
| 23 | human | 4134 | MAP4 | microtubule associated prot... | XM_024453528.1 | 43.3% | 42.3% | (many diffs) |
| 24 | human | 4134 | MAP4 | microtubule associated prot... | XM_011533705.1 | 42.3% | 42.3% | 223_273del;343_378del;1752_5654del |
| 25 | human | 4134 | MAP4 | microtubule associated prot... | XM_024453523.1 | 42% | 42% | 223_273del;343_378del;1752_5705del |
| 26 | human | 4134 | MAP4 | microtubule associated prot... | XM_011533703.2 | 42% | 42% | (many diffs) |
| 27 | human | 4134 | MAP4 | microtubule associated prot... | XM_011533708.2 | 41.7% | 41.7% | (many diffs) |
| 28 | human | 4134 | MAP4 | microtubule associated prot... | XM_011533704.2 | 41.4% | 41.4% | (many diffs) |
| 29 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006391.1 | 41.4% | 41.3% | (many diffs) |
| 30 | human | 4134 | MAP4 | microtubule associated prot... | XM_024453524.1 | 40.7% | 40.7% | (many diffs) |
| 31 | human | 4134 | MAP4 | microtubule associated prot... | XM_024453525.1 | 40.7% | 40.6% | (many diffs) |
| 32 | human | 4134 | MAP4 | microtubule associated prot... | XM_011533709.2 | 40.4% | 40.4% | (many diffs) |
| 33 | human | 4134 | MAP4 | microtubule associated prot... | XM_017006392.1 | 40.4% | 40.3% | (many diffs) |
| 34 | human | 4134 | MAP4 | microtubule associated prot... | XM_024453526.1 | 40.1% | 40.1% | (many diffs) |
| 35 | human | 4134 | MAP4 | microtubule associated prot... | XM_011533718.1 | 39.3% | 39.3% | (many diffs) |
| 36 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959529.1 | 37.1% | (many diffs) | |
| 37 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959531.1 | 35.7% | (many diffs) | |
| 38 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959532.1 | 35.3% | (many diffs) | |
| 39 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959530.1 | 35.1% | (many diffs) | |
| 40 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959533.1 | 34.4% | (many diffs) | |
| 41 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959534.1 | 33.8% | (many diffs) | |
| 42 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959519.1 | 25.9% | (many diffs) | |
| 43 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959527.1 | 25.8% | (many diffs) | |
| 44 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959525.1 | 25.7% | (many diffs) | |
| 45 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959523.1 | 25.7% | (many diffs) | |
| 46 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959521.1 | 25.6% | (many diffs) | |
| 47 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959526.1 | 24.9% | (many diffs) | |
| 48 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959524.1 | 24.8% | (many diffs) | |
| 49 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959522.1 | 24.8% | (many diffs) | |
| 50 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959520.1 | 24.7% | (many diffs) | |
| 51 | human | 4134 | MAP4 | microtubule associated prot... | XR_002959528.1 | 23.7% | (many diffs) | |
| 52 | human | 4134 | MAP4 | microtubule associated prot... | NM_030885.4 | 10% | 9.9% | 293_294delAA;295_296insGTCT;297_298ins2638 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3003
- ORF length:
- 2937
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgacctcagt cttgcagatg cattaacaga accatctcca gacattgagg 121 gagagataaa gcgggacttc attgccacac tagaggcaga ggcctttgat gatgttgtgg 181 gagaaactgt tggaaaaaca gactatattc ctctcctgga tgttgatgag aaaaccggga 241 actcagagtc aaagaagaaa ccgtgctcag aaactagcca gattgaagat actccatctt 301 ctaaaccaac actcctagcc aatggtggtc atggagtaga agggagcgat actacagggt 361 ctccaactga attccttgaa gagaaaatgg cctaccagga atacccaaat agccagaact 421 ggccagaaga taccaacttt tgtttccaac ctgagcaagt ggtcgatcct atccagactg 481 atccctttaa gatgtaccat gatgatgacc tggcagattt ggtctttccc tccagtgcga 541 cagctgatac ttcaatattt gcaggacaaa atgatccctt gaaagacagt tacggtatgt 601 ctccctgcaa cacagctgtt gtacctcagg ggtggtctgt ggaagcctta aactctccac 661 actcagagtc ctttgtttcc ccagaggctg ttgcagaacc tcctcagcca acggcagttc 721 ccttagagct agccaaggag atagaaatgg catcagaaga gaggccacca gcacaagcat 781 tggaaataat gatgggactg aagactactg acatggcacc atctaaagaa acagagatgg 841 ccctcgccaa ggacatggca ctagctacaa aaaccgaggt ggcattggct aaagatatgg 901 aatcacccac caaattagat gtgacactgg ccaaggacat gcagccatcc atggaatcag 961 atatggccct agtcaaggac atggaactac ccacagaaaa agaagtggcc ctggttaagg 1021 atgtcagatg gcccacagaa acagatgtat cttcagccaa gaatgtggta ctgcccacag 1081 aaacagaggt agccccagcc aaggatgtga cactgttgaa agaaacagag agggcatctc 1141 ctataaaaat ggacttagcc ccttccaagg acatgggacc acccaaagaa aacaagaaag 1201 aaacagagag ggcatctcct ataaaaatgg acttggctcc ttccaaggac atgggaccac 1261 ccaaagaaaa caagatagtc ccagccaagg atttggtatt actctcagaa atagaggtgg 1321 cacaggctaa tgacattata tcatccacag aaatatcctc tgctgagaag gtggctttgt 1381 cctcagaaac agaggtagcc ctggccaggg acatgacact gcccccggaa accaacgtga 1441 tcttgaccaa ggataaagca ctacctttag aagcagaggt ggccccagtc aaggacatgg 1501 ctcaactccc agaaacagaa atagccccgg ccaaggatgt ggctccgtcc acagtaaaag 1561 aagtgggctt gttgaaggac atgtctccac tatcagaaac agaaatggct ctgggcaagg 1621 atgtgactcc acctccagaa acagaagtag ttctcatcaa gaacgtatgt ctgcctccag 1681 aaatggaggt ggccctgact gaggatcagg tcccagccct caaaacagaa gctcccacca 1741 ccattggtgg gttgaataaa aaacccatga gccttgcttc aggcttagtg ccagctgccc 1801 cacccaaacg ccctgccgtc gcctctgcca ggccttccat cttaccttca aaagacgtga 1861 agccaaagcc cattgcagat gcaaaggctc ctgagaagcg ggcctcacca tccaagccag 1921 cttctgcccc agcctccaga tctgggtcca agagcactca gactgttgca aaaaccacaa 1981 cagctgctgc tgttgcctca actggcccaa gcagtaggag cccctccacg ctcctgccca 2041 agaagcccac tgccattaag actgagggaa aacctgcaga agtcaagaag atgactgcaa 2101 agtctgtacc agctgacttg agtcgcccaa agagcacctc caccagttcc atgaagaaaa 2161 ccaccactct cagtgggaca gcccccgctg caggggtggt tcccagccga gtcaaggcca 2221 cacccatgcc ctcccggccc tccacaactc ctttcataga caagaagccc acctcggcca 2281 aacccagctc caccaccccc cggctcagcc gcctggccac caatacttct gctcctgatc 2341 tgaagaatgt ccgctccaag gttggctcca cggaaaacat caagcatcag cctggaggag 2401 gccgggccaa agtagagaaa aaaacagagg cagctgctac aacccgaaag cctgaatcta 2461 atgcagtcac taaaacagcc ggcccaattg caagtgcaca gaaacaacct gcggggaaag 2521 tccagatagt ctccaaaaaa gtgagctaca gccatattca gtccaagtgt ggttccaagg 2581 acaatattaa gcatgtccct ggaggtggta atgttcagat tcagaacaag aaagtggaca 2641 tctctaaggt ctcctccaag tgtgggtcta aggctaacat caagcacaag cctggtggag 2701 gagatgtcaa gattgaaagt cagaagttga acttcaagga gaaggcccag gccaaggtgg 2761 gatccctcga taatgtgggc cacctacctg caggaggtgc tgtgaagact gagggcggtg 2821 gcagcgaggc tcctctgtgt ccgggtcccc ctgctgggga ggagccggcc atctctgagg 2881 cagcgcctga agctggcgcc cccacttcag ccagtggcct caatggccac cccaccctgt 2941 cagggggtgg tgaccaaagg gaggcccaga ccttggacag ccagatccag gagacaagca 3001 tctacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 3061 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 3121 tctggatta