Construct: ORF ccsbBroad304_10970
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002637.1_s304c1, BRDN0000401908
- Derived from:
- ccsbBroadEn_10970
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K9 (4293)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10970
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4293 | MAP3K9 | mitogen-activated protein k... | NM_001284230.1 | 99.9% | 100% | 18G>A |
| 2 | human | 4293 | MAP3K9 | mitogen-activated protein k... | NM_033141.4 | 98.7% | 98.7% | 18G>A;2026_2067del |
| 3 | human | 4293 | MAP3K9 | mitogen-activated protein k... | XM_005267683.5 | 97.8% | 97.9% | 18G>A;1842_1843ins69 |
| 4 | human | 4293 | MAP3K9 | mitogen-activated protein k... | XM_011536788.3 | 94.6% | 94.6% | 18G>A;1690_1833del;2170_2211del |
| 5 | human | 4293 | MAP3K9 | mitogen-activated protein k... | NM_001284231.1 | 69.8% | 69.2% | (many diffs) |
| 6 | human | 4293 | MAP3K9 | mitogen-activated protein k... | XM_011536792.1 | 68.4% | 68.4% | 0_1ins918;772_915del;1252_1293del |
| 7 | human | 4293 | MAP3K9 | mitogen-activated protein k... | NM_001284232.1 | 66.4% | 66.4% | (many diffs) |
| 8 | human | 4293 | MAP3K9 | mitogen-activated protein k... | XM_011536794.2 | 63.6% | 63.6% | 0_1ins1086;604_747del;1084_1125del |
| 9 | mouse | 338372 | Map3k9 | mitogen-activated protein k... | XM_006516036.3 | 89.5% | 94.7% | (many diffs) |
| 10 | mouse | 338372 | Map3k9 | mitogen-activated protein k... | NM_001174107.1 | 87.6% | 92.6% | (many diffs) |
| 11 | mouse | 338372 | Map3k9 | mitogen-activated protein k... | XM_017315122.1 | 87.5% | 91.1% | (many diffs) |
| 12 | mouse | 338372 | Map3k9 | mitogen-activated protein k... | XM_006516037.3 | 67.9% | 67.9% | (many diffs) |
| 13 | mouse | 338372 | Map3k9 | mitogen-activated protein k... | NM_177395.5 | 50.1% | 53.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3381
- ORF length:
- 3312
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagccctcc agagcacttc tcggctgcct agcgagcgcc gccgctgccg 121 ccccgccggg ggaggatgga gcaggggccg gggccgagga ggaggaggag gaggaggagg 181 aggcggcggc ggcggtgggc cccggggagc tgggctgcga cgcgccgctg ccctactgga 241 cggccgtgtt cgagtacgag gcggcgggcg aggacgagct gaccctgcgg ctgggcgacg 301 tggtggaggt gctgtccaag gactcgcagg tgtccggcga cgagggctgg tggaccgggc 361 agctgaacca gcgggtgggc atcttcccca gcaactacgt gaccccgcgc agcgccttct 421 ccagccgctg ccagcccggc ggcgaggacc ccagttgcta cccgcccatt cagttgttag 481 aaattgattt tgcggagctc accttggaag agattattgg catcgggggc tttgggaagg 541 tctatcgtgc tttctggata ggggatgagg ttgctgtgaa agcagctcgc cacgaccctg 601 atgaggacat cagccagacc atagagaatg ttcgccaaga ggccaagctc ttcgccatgc 661 tgaagcaccc caacatcatt gccctaagag gggtatgtct gaaggagccc aacctctgct 721 tggtcatgga gtttgctcgt ggaggacctt tgaatagagt gttatctggg aaaaggattc 781 ccccagacat cctggtgaat tgggctgtgc agattgccag agggatgaac tacttacatg 841 atgaggcaat tgttcccatc atccaccgcg accttaagtc cagcaacata ttgatcctcc 901 agaaggtgga gaatggagac ctgagcaaca agattctgaa gatcactgat tttggcctgg 961 ctcgggaatg gcaccgaacc accaagatga gtgcggcagg gacgtatgct tggatggcac 1021 ccgaagtcat ccgggcctcc atgttttcca aaggcagtga tgtgtggagc tatggggtgc 1081 tactttggga gttgctgact ggtgaggtgc cctttcgagg cattgatggc ttagcagtcg 1141 cttatggagt ggccatgaac aaactcgccc ttcctattcc ttctacgtgc ccagaacctt 1201 ttgccaaact catggaagac tgctggaatc ctgatcccca ctcacgacca tctttcacga 1261 atatcctgga ccagctaacc accatagagg agtctggttt ctttgaaatg cccaaggact 1321 ccttccactg cctgcaggac aactggaaac acgagattca ggagatgttt gaccaactca 1381 gggccaaaga aaaggaactt cgcacctggg aggaggagct gacgcgggct gcactgcagc 1441 agaagaacca ggaggaactg ctgcggcgtc gggagcagga gctggccgag cgggagattg 1501 acatcctgga acgggagctc aacatcatca tccaccagct gtgccaggag aagccccggg 1561 tgaagaaacg caagggcaag ttcaggaaga gccggctgaa gctcaaggat ggcaaccgca 1621 tcagcctccc ttctgatttc cagcacaagt tcacggtgca ggcctcccct accatggata 1681 aaaggaagag tcttatcaac agccgctcca gtcctcctgc aagccccacc atcattcctc 1741 gccttcgagc catccagttg acaccaggtg aaagcagcaa aacctggggc aggagctcag 1801 tcgtcccaaa ggaggaaggg gaggaggagg agaagagggc cccaaagaag aagggacgga 1861 cgtgggggcc agggacgctt ggtcagaagg agcttgcctc gggagatgaa ggatcccctc 1921 agagacgtga gaaagctaat ggtttaagta ccccatcaga atctccacat ttccacttgg 1981 gcctcaagtc cctggtagat ggatataagc agtggtcgtc cagtgccccc aacctggtga 2041 agggcccaag gagtagcccg gccctgccag ggttcaccag ccttatggag atggaggatg 2101 aggacagtga aggcccaggg agtggagaga gtcgcctaca gcattcaccc agccagtcct 2161 acctctgtat cccattccct cgtggagagg atggcgatgg cccctccagt gatggaatcc 2221 atgaggagcc caccccagtc aactcggcca cgagtacccc tcagctgacg ccaaccaaca 2281 gcctcaagcg gggcggtgcc caccaccgcc gctgcgaggt ggctctgctc ggctgtgggg 2341 ctgttctggc agccacaggc ctagggtttg acttgctgga agctggcaag tgccagctgc 2401 ttcccctgga ggagcctgag ccaccagccc gggaggagaa gaaaagacgg gagggtcttt 2461 ttcagaggtc cagccgtcct cgtcggagca ccagcccccc atcccgaaag cttttcaaga 2521 aggaggagcc catgctgttg ctaggagacc cctctgcctc cctgacgctg ctctccctct 2581 cctccatctc cgagtgcaac tccacacgct ccctgctgcg ctccgacagc gatgaaattg 2641 tcgtgtatga gatgccagtc agcccagtcg aggcccctcc cctgagtcca tgtacccaca 2701 accccctggt caatgtccga gtagagcgct tcaaacgaga tcctaaccaa tctctgactc 2761 ccacccatgt caccctcacc accccctcgc agcccagcag tcaccggcgg actccttctg 2821 atggggccct taagccagag actctcctag ccagcaggag cccctccagc aatgggttga 2881 gccccagtcc tggagcagga atgttgaaaa cccccagtcc cagccgagac ccaggtgaat 2941 tcccccgtct ccctgacccc aatgtggtct tccccccaac cccaaggcgc tggaacactc 3001 agcaggactc taccttggag agacccaaga ctctggagtt tctgcctcgg ccgcgtcctt 3061 ctgccaaccg gcaacggctg gacccttggt ggtttgtgtc ccccagccat gcccgcagca 3121 cctccccagc caacagctcc agcacagaga cgcccagcaa cctggactcc tgctttgcta 3181 gcagtagcag cactgtagag gagcggcctg gacttccagc cctgctcccg ttccaggcag 3241 ggccgctgcc cccgactgag cggacgctcc tggacctgga tgcagagggg cagagtcagg 3301 acagcaccgt gccgctgtgc agagcggaac tgaacacaca caggcctgcc ccttatgaga 3361 tccagcagga gttctggtct ttgccaactt tcttgtacaa agtggttggt aagcctatcc 3421 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 3481 ttaagtcgac aatcaacctc tggatta