Construct: ORF ccsbBroad304_10994

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007288.1_s304c1, BRDN0000411269
Derived from:
ccsbBroadEn_10994
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NEK2 (4751)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10994
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4751 NEK2 NIMA related kinase 2 NM_001204183.1 55.6% 55.2% 504T>C;639_652del;657_1152del
2 human 4751 NEK2 NIMA related kinase 2 NM_001204182.1 55% 54.6% 504T>C;639_652del;657_1164del
3 human 4751 NEK2 NIMA related kinase 2 XM_005273147.2 48.8% 48.5% 504T>C;639_652del;657_1311del
4 human 4751 NEK2 NIMA related kinase 2 NM_002497.4 48% 47.6% 504T>C;639_652del;657_1335del
5 mouse 18005 Nek2 NIMA (never in mitosis gene... XM_006497123.3 42.2% 45.6% (many diffs)
6 mouse 18005 Nek2 NIMA (never in mitosis gene... NM_010892.3 41.5% 44.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
708
ORF length:
642
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc ttcccgggct gaggactatg aagtgttgta caccattggc acaggctcct
121acggccgctg ccagaagatc cggaggaaga gtgatggcaa gatattagtt tggaaagaac
181ttgactatgg ctccatgaca gaagctgaga aacagatgct tgtttctgaa gtgaatttgc
241ttcgtgaact gaaacatcca aacatcgttc gttactatga tcggattatt gaccggacca
301atacaacact gtacattgta atggaatatt gtgaaggagg ggatctggct agtgtaatta
361caaagggaac caaggaaagg caatacttag atgaagagtt tgttcttcga gtgatgactc
421agttgactct ggccctgaag gaatgccaca gacgaagtga tggtggtcat accgtattgc
481atcgggatct gaaaccagcc aatgttttcc tggatggcaa gcaaaacgtc aagcttggag
541actttgggct agctagaata ttaaaccacg acacgagttt tgcaaaaaca tttgttggca
601caccttatta catgtctcct gaacaaatga atcgcatgtc ctacaatgag aaatcagata
661tctggtcatt gggctgcttg ctgtatgagt tatgtgcatt aatcttttac ccaactttct
721tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
781aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)