Construct: ORF ccsbBroad304_11048

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016735.1_s304c1, BRDN0000409392
Derived from:
ccsbBroadEn_11048
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPARA (5465)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11048
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5465 PPARA peroxisome proliferator act... NM_001001928.3 53.5% 49.5% (many diffs)
2 human 5465 PPARA peroxisome proliferator act... NM_001362872.2 53.5% 49.5% (many diffs)
3 human 5465 PPARA peroxisome proliferator act... NM_001362873.1 53.5% 49.5% (many diffs)
4 human 5465 PPARA peroxisome proliferator act... NM_005036.6 53.5% 49.5% (many diffs)
5 human 5465 PPARA peroxisome proliferator act... XM_005261656.3 53.5% 49.5% (many diffs)
6 human 5465 PPARA peroxisome proliferator act... XM_006724270.3 53.5% 49.5% (many diffs)
7 human 5465 PPARA peroxisome proliferator act... XM_011530239.2 53.5% 49.5% (many diffs)
8 human 5465 PPARA peroxisome proliferator act... XM_011530240.2 53.5% 49.5% (many diffs)
9 human 5465 PPARA peroxisome proliferator act... XM_011530241.2 53.5% 49.5% (many diffs)
10 human 5465 PPARA peroxisome proliferator act... XM_011530242.2 53.5% 49.5% (many diffs)
11 human 5465 PPARA peroxisome proliferator act... XM_011530243.2 53.5% 49.5% (many diffs)
12 human 5465 PPARA peroxisome proliferator act... XR_937870.2 50.1% (many diffs)
13 human 5465 PPARA peroxisome proliferator act... XR_937869.2 49.9% (many diffs)
14 human 5465 PPARA peroxisome proliferator act... XM_011530244.2 24.7% 15.2% (many diffs)
15 human 5465 PPARA peroxisome proliferator act... XM_011530245.2 24.7% 15.2% (many diffs)
16 human 5465 PPARA peroxisome proliferator act... XM_017028839.1 24.7% 15.2% (many diffs)
17 human 5465 PPARA peroxisome proliferator act... XM_024452252.1 24.7% 15.2% (many diffs)
18 human 5465 PPARA peroxisome proliferator act... XR_001755253.1 7.4% (many diffs)
19 mouse 19013 Ppara peroxisome proliferator act... XM_006520624.3 44.7% 45.7% (many diffs)
20 mouse 19013 Ppara peroxisome proliferator act... NM_001113418.1 44.6% 45.6% (many diffs)
21 mouse 19013 Ppara peroxisome proliferator act... NM_011144.6 44.6% 45.6% (many diffs)
22 mouse 19013 Ppara peroxisome proliferator act... XM_006520619.1 44.6% 45.6% (many diffs)
23 mouse 19013 Ppara peroxisome proliferator act... XM_006520620.3 44.6% 45.6% (many diffs)
24 mouse 19013 Ppara peroxisome proliferator act... XM_006520621.3 44.6% 45.6% (many diffs)
25 mouse 19013 Ppara peroxisome proliferator act... XM_006520622.3 44.6% 45.6% (many diffs)
26 mouse 19013 Ppara peroxisome proliferator act... XM_006520623.3 44.6% 45.6% (many diffs)
27 mouse 19013 Ppara peroxisome proliferator act... XM_011245516.2 44.6% 45.6% (many diffs)
28 mouse 19013 Ppara peroxisome proliferator act... XM_011245517.2 44.6% 45.6% (many diffs)
29 mouse 19013 Ppara peroxisome proliferator act... XM_011245519.2 44.6% 45.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
840
ORF length:
774
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt ggacacggaa agcccactct gccccctctc cccactcgag gccggcgatc
121tagagagccc gttatctgaa gagttcctgc aagaaatggg aaacatccaa gagatttcgc
181aatccatcgg cgaggatagt tctggaagct ttggctttac ggaataccag tatttaggaa
241gctgtcctgg ctcagatggc tcggtcatca cggacacgct ttcaccagct tcgagcccct
301cctcggtgac ttatcctgtg gtccccggca gcgtggacga gtctcccagt ggagcattga
361acatcgaatg tagaatctgc ggggacaagg cctcaggcta tcattacgga gtccacgcgt
421gtgaaggctg caagggcttc tttcggcgaa cgattcgact caagctggtg tatgacaagt
481gcgaccgcag ctgcaagatc cagaaaaaga acagaaacaa atgccagtat tgtcgatttc
541acaagtgcct ttctgtcggg atgtcacaca acgcgattcg ttttggacga atgccaagat
601ctgagaaagc aaaactgaaa gcagaaattc ttacctgtga acatgacata gaagattctg
661aaactgcaga tctcaaatct ctggccaaga gaatctacga ggcctacttg aagaacttca
721acatgaacaa ggtcaaagcc cgggtcatcc tctcaggaaa ggccagtaac aatccagtag
781gtgtttgcgg ctgttctggg ttctcttggc aacatggaac cagtgtcgta gaggacgatt
841acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
901attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
961ggatta

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