Construct: ORF ccsbBroad304_11255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007348.1_s304c1, BRDN0000408473
- Derived from:
- ccsbBroadEn_11255
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANP32A (8125)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11255
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8125 | ANP32A | acidic nuclear phosphoprote... | NM_006305.4 | 95.5% | 95.5% | 715_747del |
| 2 | human | 23520 | ANP32C | acidic nuclear phosphoprote... | NM_012403.1 | 92.8% | 86.1% | (many diffs) |
| 3 | human | 723972 | ANP32AP1 | acidic nuclear phosphoprote... | NR_003144.1 | 90.1% | (many diffs) | |
| 4 | human | 23519 | ANP32D | acidic nuclear phosphoprote... | NM_012404.3 | 51.5% | 49.1% | (many diffs) |
| 5 | mouse | 11737 | Anp32a | acidic (leucine-rich) nucle... | NM_009672.3 | 83.8% | 85.1% | (many diffs) |
| 6 | mouse | 11737 | Anp32a | acidic (leucine-rich) nucle... | XM_006510779.3 | 78.4% | 79.1% | (many diffs) |
| 7 | mouse | 11737 | Anp32a | acidic (leucine-rich) nucle... | XM_006510778.2 | 78.2% | 79.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 783
- ORF length:
- 714
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagatgggc agacggattc atttagagct gcggaacagg acgccctctg 121 atgtgaaaga acttgtcctg gacaacagtc ggtcgaatga aggcaaactc gaaggcctca 181 cagatgaatt tgaagaactg gaattcttaa gtacaatcaa cgtaggcctc acctcaatcg 241 caaacttacc aaagttaaac aaacttaaga agcttgaact aagcgataac agagtctcag 301 ggggcctgga agtattggca gaaaagtgtc cgaacctcac gcatctaaat ttaagtggca 361 acaaaattaa agacctcagc acaatagagc cactgaaaaa gttagaaaac ctcaagagct 421 tagacctttt caattgcgag gtaaccaacc tgaacgacta ccgagaaaat gtgttcaagc 481 tcctcccgca actcacatat ctcgacggct atgaccggga cgacaaggag gcccctgact 541 cggatgctga gggctacgtg gagggcctgg atgatgagga ggaggatgag gatgaggagg 601 agtatgatga agatgctcag gtagtggaag acgaggagga cgaggatgag gaggaggaag 661 gtgaagagga ggacgtgagt ggagaggagg aggaggatga agaaggttat aacgatggag 721 aggtagatga cgaggaagat gaagaagagc ttggtgaaga agaaaggggt cagaagcgaa 781 aattgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 841 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 901 tctggatta