Construct: ORF ccsbBroad304_11274
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006340.1_s304c1, BRDN0000403579
- Derived from:
- ccsbBroadEn_11274
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CUL1 (8454)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11274
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8454 | CUL1 | cullin 1 | NM_001370660.1 | 56.5% | 55.1% | (many diffs) |
| 2 | human | 8454 | CUL1 | cullin 1 | NM_001370661.1 | 56.5% | 55.1% | (many diffs) |
| 3 | human | 8454 | CUL1 | cullin 1 | NM_001370662.1 | 56.5% | 55.1% | (many diffs) |
| 4 | human | 8454 | CUL1 | cullin 1 | NM_001370663.1 | 56.5% | 55.1% | (many diffs) |
| 5 | human | 8454 | CUL1 | cullin 1 | NM_001370664.1 | 56.5% | 55.1% | (many diffs) |
| 6 | human | 8454 | CUL1 | cullin 1 | NM_003592.3 | 56.5% | 55.1% | (many diffs) |
| 7 | mouse | 26965 | Cul1 | cullin 1 | NM_012042.3 | 52% | 55.1% | (many diffs) |
| 8 | mouse | 26965 | Cul1 | cullin 1 | XM_006506188.3 | 52% | 55.1% | (many diffs) |
| 9 | mouse | 26965 | Cul1 | cullin 1 | XM_006506189.3 | 52% | 55.1% | (many diffs) |
| 10 | mouse | 26965 | Cul1 | cullin 1 | XM_006506190.3 | 52% | 55.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1452
- ORF length:
- 1386
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca agctcggaat caatatttag ttttgagtgt ggagaataga ttgttttttt 121 cttttatata tataaacaaa atgtcctatg tgtttcaacg atggtaccaa aagtatttct 181 tgctcttatc tcttaacttt gaaaaggacc ccaaaatgta tgtacagaca gtgcttgatg 241 ttcatagaaa atacaatgcc ctggtaatgt ctgcattcaa caatgacgct ggctttgtgg 301 ctgctcttga taaggcttgt ggtcgcttca taaacaacaa cgcggttacc aagatggccc 361 aatcatccag taaatcccct gagttgctgg ctcgatactg tgactccttg ttgaagaaaa 421 gttccaagaa cccagaggag gcagaactag aagacacact caatcaagtg atggttgtct 481 tcaagtacat agaagacaaa gacgtatttc agaagttcta tgcgaagatg ctcgccaaga 541 ggctcgtcca ccagaacagt gcaagtgacg atgccgaagc cagcatgatc tccaagttaa 601 agcaagcttg cgggttcgag tacacctcta aacttcagcg catgtttcaa gacattggcg 661 tgagcaaaga tctgaacgag caattcaaaa agcacttgac aaactcagaa cccctagact 721 tggatttcag cattcaagtg ctgagctccg ggtcctggcc cttccagcag ttttgtacat 781 ttgccttgcc gtcagagttg gaacgtagtt atcagcgatt cacagctttc tacgccagcc 841 gccacagtgg ccgaaaattg acgtggttat atcagttgtc taaaggagaa ttggtaacta 901 actgcttcaa aaacagatat actttgcagg cgtcgacatt ccagatggct atcctgcttc 961 agtacaacac ggaagatgcc tacactgtgc agcagctgac cgacagcact caaattaaaa 1021 tggacatttt ggcgcaagtt ttacagattt tattaaagtc gaagctattg gtcttggaag 1081 atgaaaatgc aaatgttgat gaggtggaat tgaagccaga taccttaata aaattatatc 1141 ttggttataa aaataagaaa ttaagggtta acatcaatgt gccaatgaaa accgaacaga 1201 agcaggaaca agaaaccaca cacaaaaaca tcgaggaaga ccgcaaacta ctgattcagg 1261 cggccatcgt gagaatcatg aagatgagga aggttctgaa acaccagcag ttacttggcg 1321 aggtcctcac tcagctgtcc tccaggttca aacctcgagt ccctgtgatc aagaaatgca 1381 ttgacattct aattgagaaa gaatatttgg agcgagtgga tggtgaaaag gacacctaca 1441 gttacttggc ttacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1501 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1561 caatcaacct ctggatta