Construct: ORF ccsbBroad304_11291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012481.1_s304c1, BRDN0000400431
- Derived from:
- ccsbBroadEn_11291
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUMB (8650)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11291
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005745.1 | 22.8% | 22.8% | 1_1371del |
| 2 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005744.1 | 22.3% | 22.3% | 1_1404del |
| 3 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001320114.1 | 22.3% | 22.3% | 1_1404del |
| 4 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_003744.5 | 21% | 21% | 1_1515del |
| 5 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005743.1 | 20.7% | 20.7% | 1_1548del |
| 6 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001272056.1 | 20.1% | 20.4% | (many diffs) |
| 7 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_010949.2 | 19.8% | 20% | (many diffs) |
| 8 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001272055.1 | 18.6% | 18.8% | (many diffs) |
| 9 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515578.1 | 18.6% | 18.8% | (many diffs) |
| 10 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001136075.2 | 18.3% | 18.5% | (many diffs) |
| 11 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515576.1 | 18.3% | 18.5% | (many diffs) |
| 12 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515577.2 | 18.3% | 18.5% | (many diffs) |
| 13 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_011244012.2 | 18.3% | 18.5% | (many diffs) |
| 14 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515575.2 | 17.5% | 17.7% | (many diffs) |
| 15 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515574.2 | 17.3% | 17.5% | (many diffs) |
| 16 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515573.2 | 16.4% | 16.5% | (many diffs) |
| 17 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515572.2 | 16.1% | 16.3% | (many diffs) |
| 18 | mouse | 18222 | Numb | numb homolog (Drosophila) | NR_073563.1 | 10.1% | (many diffs) | |
| 19 | mouse | 18222 | Numb | numb homolog (Drosophila) | NR_073564.1 | 9.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ctatccagcc cctaatgtgc ctgtggtggg catcactccc tcccagatgg 121 tggccaacgt atttggcact gcaggccacc ctcaggctgc ccatccccat cagtcaccca 181 gcctggtcag gcagcagaca ttccctcact acgaggcaag cagtgctacc accagtccct 241 tctttaagcc tcctgctcag cacctcaacg gttctgcagc tttcaatggt gtagatgatg 301 gcaggttggc ctcagcagac aggcatacag aggttcctac aggcacctgc ccagtggatc 361 cttttgaagc ccagtgggct gcattagaaa ataagtccaa gcagcgtact aatccctccc 421 ctaccaaccc tttctccagt gacttacaga agacgtttga aattgaactt tacccaactt 481 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 541 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta