Transcript: Mouse NM_001272056.1

Mus musculus numb homolog (Drosophila) (Numb), transcript variant 4, mRNA.

Source:
NCBI, updated 2017-06-14
Taxon:
Mus musculus (mouse)
Gene:
Numb (18222)
Length:
3432
CDS:
246..2027

Additional Resources:

NCBI RefSeq record:
NM_001272056.1
NBCI Gene record:
Numb (18222)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001272056.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000105735 GCAGCCTGTTTAGAGCGTAAA pLKO.1 699 CDS 100% 10.800 15.120 N Numb n/a
2 TRCN0000327233 GCAGCCTGTTTAGAGCGTAAA pLKO_005 699 CDS 100% 10.800 15.120 N Numb n/a
3 TRCN0000105736 GCTGGTTAGAAGAAGTGTCAA pLKO.1 1396 CDS 100% 4.950 3.960 N Numb n/a
4 TRCN0000327309 GCTGGTTAGAAGAAGTGTCAA pLKO_005 1396 CDS 100% 4.950 3.960 N Numb n/a
5 TRCN0000105739 CAGCAGACATTCCCTCAATAT pLKO.1 1746 CDS 100% 13.200 9.240 N Numb n/a
6 TRCN0000363599 CAGCAGACATTCCCTCAATAT pLKO_005 1746 CDS 100% 13.200 9.240 N Numb n/a
7 TRCN0000105738 GCCAGAAGATGTCACCCTTTA pLKO.1 1063 CDS 100% 10.800 7.560 N Numb n/a
8 TRCN0000327236 GCCAGAAGATGTCACCCTTTA pLKO_005 1063 CDS 100% 10.800 7.560 N Numb n/a
9 TRCN0000105737 GCGATGGATCTGTCATTGTTT pLKO.1 623 CDS 100% 5.625 3.938 N Numb n/a
10 TRCN0000327235 GCGATGGATCTGTCATTGTTT pLKO_005 623 CDS 100% 5.625 3.938 N Numb n/a
11 TRCN0000007225 GCAGCTTTCAATGGTGTAGAT pLKO.1 1830 CDS 100% 4.950 6.930 N NUMB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001272056.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491413 CTGCATACTTAGTCTAAACTTGTC pLX_317 12.9% 88.9% 94% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000489619 AATGGCTCTCTTGTCCGGCATGCA pLX_317 19.6% 84.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000489234 AACTCGAACCGCAACTACAAGGAA pLX_317 18.8% 81% 85.5% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_11292 pDONR223 100% 35.7% 36.1% None (many diffs) n/a
5 ccsbBroad304_11292 pLX_304 0% 35.7% 36.1% V5 (many diffs) n/a
6 TRCN0000474826 GACTGGCGCTTGCTACGCGTTACG pLX_317 54.4% 35.7% 36.1% V5 (many diffs) n/a
7 ccsbBroadEn_11291 pDONR223 100% 20.1% 20.4% None (many diffs) n/a
8 ccsbBroad304_11291 pLX_304 0% 20.1% 20.4% V5 (many diffs) n/a
9 TRCN0000466523 ATTTCTTGGATTTTTATCGGCCAC pLX_317 93.8% 20.1% 20.4% V5 (many diffs) n/a
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