Construct: ORF ccsbBroad304_11323
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001268.1_s304c1, BRDN0000405329
- Derived from:
- ccsbBroadEn_11323
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P4HA2 (8974)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11323
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001017974.2 | 94.5% | 94.5% | 1_87del |
| 2 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001142598.2 | 94.5% | 94.5% | 1_87del |
| 3 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001365678.2 | 94.5% | 94.5% | 1_87del |
| 4 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001365679.2 | 94.5% | 94.5% | 1_87del |
| 5 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001365680.2 | 94.5% | 94.5% | 1_87del |
| 6 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001142599.2 | 92.2% | 92.3% | (many diffs) |
| 7 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001365677.2 | 92.2% | 92.3% | (many diffs) |
| 8 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_004199.3 | 92.2% | 92.3% | (many diffs) |
| 9 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_005272117.4 | 92.2% | 92.3% | (many diffs) |
| 10 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_005272118.4 | 92.2% | 92.3% | (many diffs) |
| 11 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_005272119.4 | 92.2% | 92.3% | (many diffs) |
| 12 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_005272120.4 | 92.2% | 92.3% | (many diffs) |
| 13 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_006714728.3 | 92.2% | 92.3% | (many diffs) |
| 14 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001365681.2 | 83.4% | 83.4% | 1_87del;903_904ins177 |
| 15 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_024446256.1 | 83.4% | 83.4% | 1_87del;903_904ins177 |
| 16 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_024446255.1 | 81.2% | 81.3% | (many diffs) |
| 17 | mouse | 18452 | P4ha2 | procollagen-proline, 2-oxog... | NM_001136076.2 | 82.3% | 88.4% | (many diffs) |
| 18 | mouse | 18452 | P4ha2 | procollagen-proline, 2-oxog... | NM_011031.2 | 80.8% | 86.2% | (many diffs) |
| 19 | mouse | 18452 | P4ha2 | procollagen-proline, 2-oxog... | XM_006532452.2 | 79.1% | 84.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1581
- ORF length:
- 1512
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gactgacctg atttatgcag agaaagagct ggtgcagtct ctgaaagagt 121 acatccttgt ggaggaagcc aagctttcca agattaagag ctgggccaac aaaatggaag 181 ccttgactag caagtcagct gctgatgctg agggctacct ggctcaccct gtgaatgcct 241 acaaactggt gaagcggcta aacacagact ggcctgcgct ggaggacctt gtcctgcagg 301 actcagctgc aggttttatc gccaacctct ctgtgcagcg gcagttcttc cccactgatg 361 aggacgagat aggagctgcc aaagccctga tgagacttca ggacacatac aggctggacc 421 caggcacaat ttccagaggg gaacttccag gaaccaagta ccaggcaatg ctgagtgtgg 481 atgactgctt tgggatgggc cgctcggcct acaatgaagg ggactattat catacggtgt 541 tgtggatgga gcaggtgcta aagcagcttg atgccgggga ggaggccacc acaaccaagt 601 cacaggtgct ggactacctc agctatgctg tcttccagtt gggtgatctg caccgtgccc 661 tggagctcac ccgccgcctg ctctcccttg acccaagcca cgaacgagct ggagggaatc 721 tgcggtactt tgagcagtta ttggaggaag agagagaaaa aacgttaaca aatcagacag 781 aagctgagct agcaacccca gaaggcatct atgagaggcc tgtggactac ctgcctgaga 841 gggatgttta cgagagcctc tgtcgtgggg agggtgtcaa actgacaccc cgtagacaga 901 agaggctttt ctgtaggtac caccatggca acagggcccc acagctgctc attgccccct 961 tcaaagagga ggacgagtgg gacagcccgc acatcgtcag gtactacgat gtcatgtctg 1021 atgaggaaat cgagaggatc aaggagatcg caaaacctaa acttgcacga gccaccgttc 1081 gtgatcccaa gacaggagtc ctcactgtcg ccagctaccg ggtttccaaa agctcctggc 1141 tagaggaaga tgatgaccct gttgtggccc gagtaaatcg tcggatgcag catatcacag 1201 ggttaacagt aaagactgca gaattgttac aggttgcaaa ttatggagtg ggaggacagt 1261 atgaaccgca cttcgacttc tctaggcgac cttttgacag cggcctcaaa acagagggga 1321 ataggttagc gacgtttctt aactacatga gtgatgtaga agctggtggt gccaccgtct 1381 tccctgatct gggggctgca atttggccta agaagggtac agctgtgttc tggtacaacc 1441 tcttgcggag cggggaaggt gactaccgaa caagacatgc tgcctgccct gtgcttgtgg 1501 gctgcaagtg ggtctccaat aagtggttcc atgaacgagg acaggagttc ttgagacctt 1561 gtggatcaac agaagttgac ttgccaactt tcttgtacaa agtggttggt aagcctatcc 1621 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1681 ttaagtcgac aatcaacctc tggatta