Construct: ORF ccsbBroad304_11419
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004717.1_s304c1, BRDN0000401804
- Derived from:
- ccsbBroadEn_11419
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC6 (9829)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11419
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9829 | DNAJC6 | DnaJ heat shock protein fam... | NM_001256864.2 | 99.9% | 100% | 1500G>A;2010A>C |
| 2 | human | 9829 | DNAJC6 | DnaJ heat shock protein fam... | NM_014787.4 | 93.2% | 93.4% | (many diffs) |
| 3 | human | 9829 | DNAJC6 | DnaJ heat shock protein fam... | NM_001256865.1 | 92.7% | 92.7% | 0_1ins210;1290G>A;1800A>C |
| 4 | mouse | 72685 | Dnajc6 | DnaJ heat shock protein fam... | NM_001164583.1 | 87.5% | 92.8% | (many diffs) |
| 5 | mouse | 72685 | Dnajc6 | DnaJ heat shock protein fam... | XM_006503421.3 | 84.1% | 88.7% | (many diffs) |
| 6 | mouse | 72685 | Dnajc6 | DnaJ heat shock protein fam... | NM_001164584.1 | 83.4% | 88.6% | (many diffs) |
| 7 | mouse | 72685 | Dnajc6 | DnaJ heat shock protein fam... | NM_001164585.1 | 81.3% | 87.2% | (many diffs) |
| 8 | mouse | 72685 | Dnajc6 | DnaJ heat shock protein fam... | NM_198412.2 | 81.3% | 87.2% | (many diffs) |
| 9 | mouse | 72685 | Dnajc6 | DnaJ heat shock protein fam... | XM_017320393.1 | 81.3% | 87.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2979
- ORF length:
- 2910
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcctcctc gggagctacc ggaaaaagac cagcaacgat ggttatgaat 121 ctttgcagct ggtggacagt aacggggact taagtgcggg aagcggcggg gttggcggca 181 agcagagagt gaacgccggg gcagcggcgc ggagtcccgc ccgacagcct ccggaccgcg 241 ccagcaccat ggacagctca ggtgcctcat ctccagacat ggagcccagc tatgggggag 301 gtctctttga catggtaaaa ggaggtgcag ggaggctctt tagtaaccta aaggacaact 361 tgaaagacac cctcaaagac acatcttcta gagtgataca atctgtgacc agctacacaa 421 agggagattt agacttcact tatgttacct ccagaattat tgtgatgtcc tttcctctgg 481 acaatgttga cataggattc aggaatcagg ttgatgacat tcgaagcttt ttggattcca 541 gacatcttga ccactacaca gtatacaatc tgtcacctaa gtcttatcga actgccaagt 601 ttcacagccg ggtctcagaa tgcagttggc ccattaggca ggctcccagt ctgcacaacc 661 tttttgctgt gtgtcggaat atgtataact ggctactgca gaatcccaaa aatgtctgtg 721 ttgtccactg cttggatgga cgggcggcat catcaattct ggttggtgct atgttcattt 781 tctgtaatct ctactctact cctggcccag ccattcgatt gctatatgca aagcgaccag 841 gaattggact ttcaccatcc cataggagat acctgggcta tatgtgtgac ctactggcag 901 acaagcccta ccgccctcac ttcaagcctc tcacaattaa gtcgatcact gtcagtccaa 961 tacccttttt caacaaacag aggaatggat gtcgccctta ctgtgatgta ctcattggag 1021 aaaccaaaat atattcgact tgcacagatt ttgaacgaat gaaagaatat cgtgtccaag 1081 atggaaaaat cttcattccc ttgaacatca ctgtgcaagg agacgtggtt gtttccatgt 1141 atcacttgag gtcaaccatt gggagccggc tacaggctaa ggtgaccaac acacagatat 1201 tccagcttca gtttcacact ggattcatac cactggacac aacagtttta aagttcacca 1261 agcctgagtt agatgcatgt gatgtaccag aaaaatatcc tcagctattt caggtgacac 1321 tggatgtaga actacagccc catgacaaag taatagactt aactccacca tgggaacatt 1381 actgcacaaa agatgtcaat cccagcatcc tcttctcttc tcaccaggaa catcaagata 1441 cgctggcctt aggaggacag gctccaatag atatccctcc agacaacccc aggcattacg 1501 gacaaagtgg tttctttgcc tctctctgtt ggcaagatca gaaatcggag aagtcattct 1561 gtgaggaaga ccacgctgcc ctagtgaatc aggaaagtga gcaatcagat gatgaacttc 1621 tgacactttc cagtccgcat ggcaatgcca atggtgacaa gcctcatgga gtcaagaagc 1681 ccagcaaaaa gcagcaggag ccagcagccc ctccaccccc tgaggatgtg gaccttttgg 1741 gcctggaagg gtctgcaatg agtaacagct tctctccgcc agcggctcct cccaccaatt 1801 ctgaactact gagtgacctg tttgggggtg gaggtgcagc tggtcccacc caggctggac 1861 agtcaggagt ggaagatgtg tttcatccta gtggacctgc gtctacccag tcaacaccac 1921 gccgctctgc cacctccacc tctgcgtctc caaccctaag agtgggagaa ggtgccacct 1981 ttgacccatt tggagcacct tctaaaccat caggtcagga tttgctgggt tcttttctga 2041 acacatccag tgcttccagt gacccctttc tccagcccac aagaagtcct tcgcccacag 2101 tacatgcttc tagtacgcct gctgtgaaca ttcagccaga tgtttctgga ggttgggact 2161 ggcatgctaa accaggaggc tttggaatgg gaagcaagtc agctgccacc agcccaaccg 2221 gatcctcgca tggtactccc acccatcaaa gcaaacccca gactctggat ccttttgccg 2281 accttgggac actaggtagt tcttcctttg ccagcaaacc caccacacca actggattgg 2341 gtggaggatt cccgcctctc agctcgccac agaaggcgtc tccccagcct atgggtggcg 2401 ggtggcagca gggaggtgcc tacaactggc agcagccaca gcctaagcct cagcccagca 2461 tgccccactc ctctccccag aaccgaccca actacaacgt gagcttctca gccatgcctg 2521 ggggccagaa cgaacgtggg aaaggatcaa gtaatttgga agggaaacaa aaagcagctg 2581 attttgaaga cctactctct ggtcaaggtt tcaatgctca caaagacaaa aaggggcctc 2641 ggacaatagc tgagatgaga aaggaggaaa tggccaagga aatggatcct gagaaattaa 2701 agattctgga atggattgaa ggcaaagaaa gaaatatcag agcccttctt tccacgatgc 2761 ataccgtact atgggctggg gagaccaagt ggaaaccagt tggcatggca gacctggtaa 2821 caccagagca ggtgaagaag gtgtacagga aggctgtcct ggtggtgcac ccagataaag 2881 ctactgggca accctatgaa caatacgcaa agatgatttt catggagctc aatgatgcct 2941 ggtctgaatt tgaaaaccaa ggccaaaagc ccttatattt gccaactttc ttgtacaaag 3001 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 3061 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta