Construct: ORF ccsbBroad304_11447
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010539.1_s304c1, BRDN0000407264
- Derived from:
- ccsbBroadEn_11447
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH2B3 (10019)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11447
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100289019 | SLC25A25-AS1 | SLC25A25 antisense RNA 1 | NR_033374.1 | 3.4% | (many diffs) | |
| 2 | human | 10019 | SH2B3 | SH2B adaptor protein 3 | XR_002957278.1 | 2.7% | 1_3974del;4128_5649del | |
| 3 | human | 10019 | SH2B3 | SH2B adaptor protein 3 | XR_001748536.1 | 2.6% | 1_4004del;4158_5679del | |
| 4 | human | 10019 | SH2B3 | SH2B adaptor protein 3 | XR_001748535.1 | 2.6% | 1_4098del;4252_5773del | |
| 5 | human | 3824 | KLRD1 | killer cell lectin like rec... | NR_147040.2 | .8% | (many diffs) | |
| 6 | human | 3824 | KLRD1 | killer cell lectin like rec... | NR_147039.2 | .8% | (many diffs) | |
| 7 | human | 3824 | KLRD1 | killer cell lectin like rec... | NR_147038.2 | .8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 219
- ORF length:
- 153
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gtttctcttg tcaaccgggc tggagtgcag tggtgcaatc ttggctcact 121 gcaacctcca ccttcctggt tcaagcgatt ctgcctcgac ctctcaagta gctgggatta 181 caagcaccag ccaccatgcc tggctaattt tgtattttta cccaactttc ttgtacaaag 241 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 301 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta