Construct: ORF ccsbBroad304_11466

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003484.1_s304c1, BRDN0000400224
Derived from:
ccsbBroadEn_11466
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TNK2 (10188)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11466
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10188 TNK2 tyrosine kinase non receptor 2 NM_005781.4 48.7% 47.4% (many diffs)
2 human 10188 TNK2 tyrosine kinase non receptor 2 NM_001308046.1 48.6% 47.3% (many diffs)
3 human 10188 TNK2 tyrosine kinase non receptor 2 NM_001010938.2 48.5% 47.3% (many diffs)
4 human 10188 TNK2 tyrosine kinase non receptor 2 XM_005269270.3 48.1% 46.9% (many diffs)
5 human 10188 TNK2 tyrosine kinase non receptor 2 XM_011512320.1 48.1% 46.9% (many diffs)
6 human 10188 TNK2 tyrosine kinase non receptor 2 XM_017005510.1 48.1% 46.9% (many diffs)
7 human 10188 TNK2 tyrosine kinase non receptor 2 XM_024453293.1 48.1% 46.9% (many diffs)
8 human 10188 TNK2 tyrosine kinase non receptor 2 XM_024453294.1 48.1% 46.9% (many diffs)
9 human 10188 TNK2 tyrosine kinase non receptor 2 XM_024453295.1 48.1% 46.9% (many diffs)
10 human 10188 TNK2 tyrosine kinase non receptor 2 XM_024453292.1 47.4% 46.2% (many diffs)
11 human 10188 TNK2 tyrosine kinase non receptor 2 XM_017005509.1 47.3% 46% (many diffs)
12 human 10188 TNK2 tyrosine kinase non receptor 2 XM_017005508.1 47.1% 45.9% (many diffs)
13 human 10188 TNK2 tyrosine kinase non receptor 2 XM_011512318.2 46.8% 45.6% (many diffs)
14 human 10188 TNK2 tyrosine kinase non receptor 2 XM_005269268.3 45.5% 44.3% (many diffs)
15 human 10188 TNK2 tyrosine kinase non receptor 2 XM_024453291.1 45.4% 44.3% (many diffs)
16 human 10188 TNK2 tyrosine kinase non receptor 2 XM_011512317.3 41.7% 40.6% (many diffs)
17 human 10188 TNK2 tyrosine kinase non receptor 2 XM_011512321.2 41% 39.6% (many diffs)
18 human 10188 TNK2 tyrosine kinase non receptor 2 XM_005269274.3 25.7% 24.4% (many diffs)
19 human 10188 TNK2 tyrosine kinase non receptor 2 XM_005269275.3 18.7% 17.3% (many diffs)
20 mouse 51789 Tnk2 tyrosine kinase, non-recept... NM_001110147.1 45% 47.9% (many diffs)
21 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_006522340.3 43.7% 46.4% (many diffs)
22 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_017317066.1 43.6% 46.4% (many diffs)
23 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_006522339.1 43.6% 45.9% (many diffs)
24 mouse 51789 Tnk2 tyrosine kinase, non-recept... NM_016788.3 43.2% 45.5% (many diffs)
25 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_006522338.3 43.2% 45.5% (many diffs)
26 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_006522337.3 42.5% 45.1% (many diffs)
27 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_017317065.1 42.4% 45.1% (many diffs)
28 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_006522336.3 42.4% 45% (many diffs)
29 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_006522335.1 42.3% 44.5% (many diffs)
30 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_006522334.3 42% 44.3% (many diffs)
31 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_006522333.3 42% 44.2% (many diffs)
32 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_006522332.3 42% 44.2% (many diffs)
33 mouse 51789 Tnk2 tyrosine kinase, non-recept... XM_011245946.1 29.1% 30.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1650
ORF length:
1584
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca gccagaggag ggcacaggct ggctgctgga gctgctgtcc gaggtgcagc
121tgcaacagta cttcctgcgg ctccgagacg acctcaacgt cacccgcctg tcccactttg
181agtacgtcaa gaatgaggac ctggagaaga tcggcatggg tcggcctggc cagcggcggc
241tgtgggaggc tgtgaagagg aggaaggcct tgtgcaaacg caagtcgtgg atgagtaagg
301tgttcagtgg aaagcgactg gaggctgagt tcccacctca tcactctcag agcaccttcc
361ggaagacctc gcccgcccct gggggcccag caggggaggg gcccctgcag agcctcacct
421gcctcattgg ggagaaggac ctgcgcctcc tggagaagct gggtgatggt tcctttggcg
481tggtgcgcag gggcgagtgg gacgcgccct cagggaagac ggtgagtgtg gctgtgaagt
541gcctgaagcc cgatgtcctg agccagccag aagccatgga cgacttcatc cgggaggtca
601atgccatgca ctcgctcgac caccgaaacc tcatccgcct ctacggggtg gtgctcacgc
661cgcccatgaa gatggtgaca gagctggcac ctctgggatc gttgttggac cggctacgta
721agcaccaggg ccacttcctc ctggggactc tgagccgcta cgctgtgcag gtggctgagg
781gcatgggcta cctggagtcc aagcgcttta ttcaccgtga cctggctgcc cgcaatctgc
841tgttggctac ccgcgacctg gtcaagatcg gggactttgg gctgatgcga gcactacctc
901agaatgacga ccattacgtc atgcaggaac atcgcaaggt gcccttcgcc tggtgtgccc
961ccgagagcct gaagacacgt accttctccc atgccagcga cacctggatg ttcggggtga
1021cactgtggga aatgttcacc tacggccagg agccctggat cggcctcaac ggcagtcaga
1081tcctgcataa gatcgacaag gagggggagc ggctgccccg gcccgaggac tgtccccagg
1141acatctacaa cgtcatggtc cagtgctggg ctcacaagcc agaggacaga cccacgtttg
1201tggccctgcg ggacttcctg ctggaggccc agcccacaga catgcgggcc cttcaggact
1261ttgaggaacc ggacaagctg cacatccaga tgaatgatgt catcaccgtc atcgagggaa
1321gggccgagaa ctactggtgg cgtggccaga acacacggac gctgtgtgtg gggcccttcc
1381ctcgcaacgt ggtgacctcc gtggccggcc tgtcggccca ggacatcagc cagcccctgc
1441agaacagctt catccacaca gggcatggcg acagtgaccc ccgccactgc tggggcttcc
1501cggacaggat tgacgagtgt cctttctctg ccttctctcc aggacacccc ccagctgaga
1561cgtgcggtca ggttctgtgg actgggaggc gggaggcctg tgcttcagac ccaagactac
1621acccggtttc ctccaggacg aaggggctct gcccaacttt cttgtacaaa gtggttggta
1681agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1741ggtggcgcgt taagtcgaca atcaacctct ggatta

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