Transcript: Human XM_011512317.3

PREDICTED: Homo sapiens tyrosine kinase non receptor 2 (TNK2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TNK2 (10188)
Length:
4887
CDS:
411..4070

Additional Resources:

NCBI RefSeq record:
XM_011512317.3
NBCI Gene record:
TNK2 (10188)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149049 GTTCAGTGGAAAGCGACTGG pXPR_003 AGG 628 18% 6 0.7196 TNK2 TNK2 75584
2 BRDN0001148153 TCCCGCAGGGCCACAAACGT pXPR_003 GGG 1506 43% 10 0.502 TNK2 TNK2 75582
3 BRDN0001148514 CGGTCCAACAACGATCCCAG pXPR_003 AGG 1005 28% 8 0.4684 TNK2 TNK2 75583
4 BRDN0001149191 GGGGCCCTTCCCTCGCAACG pXPR_003 TGG 1696 48% 12 -0.3299 TNK2 TNK2 75585
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011512317.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195647 CCCACTTTGAGTACGTCAAGA pLKO.1 1009 CDS 100% 4.950 6.930 N TNK2 n/a
2 TRCN0000002038 GTCGTGGATGAGTAAGGTGTT pLKO.1 1121 CDS 100% 4.050 5.670 N TNK2 n/a
3 TRCN0000002040 TGACGAACTGTATCTGGGAAA pLKO.1 2348 CDS 100% 4.050 5.670 N TNK2 n/a
4 TRCN0000197093 GCCTTACCATGGACTTGAATG pLKO.1 4583 3UTR 100% 10.800 7.560 N TNK2 n/a
5 TRCN0000195521 CATTACGTCATGCAGGAACAT pLKO.1 1749 CDS 100% 4.950 3.465 N TNK2 n/a
6 TRCN0000199131 CCAGACCAACTACGCCTTTGT pLKO.1 2945 CDS 100% 4.950 3.465 N TNK2 n/a
7 TRCN0000002041 CTGCATAAGATCGACAAGGAG pLKO.1 1920 CDS 100% 2.640 1.848 N TNK2 n/a
8 TRCN0000199020 CCGGACAGGATTGACGAACTG pLKO.1 2337 CDS 100% 1.350 0.945 N TNK2 n/a
9 TRCN0000195330 CCAGATGAATGATGTCATCAC pLKO.1 2123 CDS 100% 0.405 0.284 N TNK2 n/a
10 TRCN0000199936 GCGCTGTCCCTGCACACTTTG pLKO.1 4254 3UTR 100% 0.000 0.000 N TNK2 n/a
11 TRCN0000002039 TGCTTCCTCTTCCACCCAATT pLKO.1 4759 3UTR 100% 10.800 6.480 N TNK2 n/a
12 TRCN0000002042 CAACTTCTCCACCAACAACAG pLKO.1 3710 CDS 100% 4.050 2.430 N TNK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011512317.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488774 ATCGAATTATCCTTTTGCTGGTCT pLX_317 11.4% 85.1% 85.1% V5 (not translated due to prior stop codon) 1_492del;2035_2079del;3429_3434delGCTGCA n/a
2 ccsbBroadEn_11466 pDONR223 100% 41.7% 40.6% None (many diffs) n/a
3 ccsbBroad304_11466 pLX_304 0% 41.7% 40.6% V5 (many diffs) n/a
4 TRCN0000466880 CTTTTGTGTCCATAAGCAAACTTA pLX_317 24.8% 41.7% 40.6% V5 (many diffs) n/a
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