Transcript: Human XM_017005509.1

PREDICTED: Homo sapiens tyrosine kinase non receptor 2 (TNK2), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TNK2 (10188)
Length:
3387
CDS:
178..3387

Additional Resources:

NCBI RefSeq record:
XM_017005509.1
NBCI Gene record:
TNK2 (10188)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149049 GTTCAGTGGAAAGCGACTGG pXPR_003 AGG 349 11% 6 0.6499 TNK2 TNK2 75584
2 BRDN0001148153 TCCCGCAGGGCCACAAACGT pXPR_003 GGG 1227 38% 10 0.4894 TNK2 TNK2 75582
3 BRDN0001148514 CGGTCCAACAACGATCCCAG pXPR_003 AGG 726 23% 8 0.4563 TNK2 TNK2 75583
4 BRDN0001149191 GGGGCCCTTCCCTCGCAACG pXPR_003 TGG 1417 44% 12 -0.3644 TNK2 TNK2 75585
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017005509.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195647 CCCACTTTGAGTACGTCAAGA pLKO.1 380 CDS 100% 4.950 6.930 N TNK2 n/a
2 TRCN0000002038 GTCGTGGATGAGTAAGGTGTT pLKO.1 492 CDS 100% 4.050 5.670 N TNK2 n/a
3 TRCN0000002040 TGACGAACTGTATCTGGGAAA pLKO.1 1719 CDS 100% 4.050 5.670 N TNK2 n/a
4 TRCN0000195521 CATTACGTCATGCAGGAACAT pLKO.1 1120 CDS 100% 4.950 3.465 N TNK2 n/a
5 TRCN0000199131 CCAGACCAACTACGCCTTTGT pLKO.1 2271 CDS 100% 4.950 3.465 N TNK2 n/a
6 TRCN0000002041 CTGCATAAGATCGACAAGGAG pLKO.1 1291 CDS 100% 2.640 1.848 N TNK2 n/a
7 TRCN0000199020 CCGGACAGGATTGACGAACTG pLKO.1 1708 CDS 100% 1.350 0.945 N TNK2 n/a
8 TRCN0000195330 CCAGATGAATGATGTCATCAC pLKO.1 1494 CDS 100% 0.405 0.284 N TNK2 n/a
9 TRCN0000002042 CAACTTCTCCACCAACAACAG pLKO.1 3036 CDS 100% 4.050 2.430 N TNK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017005509.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488774 ATCGAATTATCCTTTTGCTGGTCT pLX_317 11.4% 96.9% 96.9% V5 (not translated due to prior stop codon) 1_96del;3207_3208insCGC n/a
2 ccsbBroadEn_11466 pDONR223 100% 47.3% 46% None (many diffs) n/a
3 ccsbBroad304_11466 pLX_304 0% 47.3% 46% V5 (many diffs) n/a
4 TRCN0000466880 CTTTTGTGTCCATAAGCAAACTTA pLX_317 24.8% 47.3% 46% V5 (many diffs) n/a
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