Construct: ORF ccsbBroad304_11469
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004302.1_s304c1, BRDN0000462304
- Derived from:
- ccsbBroadEn_11469
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PATJ (10207)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11469
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_006710278.4 | 90.4% | 89.9% | (many diffs) |
| 2 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_005270347.2 | 90.2% | 89.7% | (many diffs) |
| 3 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_011540469.3 | 87.2% | 86.7% | (many diffs) |
| 4 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_011540470.2 | 80.2% | 79.9% | (many diffs) |
| 5 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XR_001736900.2 | 80% | (many diffs) | |
| 6 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_011540468.3 | 77.4% | 76.9% | (many diffs) |
| 7 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XR_002957157.1 | 72.4% | (many diffs) | |
| 8 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_024448642.1 | 65.2% | 64.8% | (many diffs) |
| 9 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | NM_176877.4 | 65.1% | 64.7% | (many diffs) |
| 10 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_016999999.2 | 63.2% | 62.9% | (many diffs) |
| 11 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_011540467.3 | 63.2% | 62.8% | (many diffs) |
| 12 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_016999998.2 | 62.4% | 62% | (many diffs) |
| 13 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | NM_001350145.2 | 62.3% | 61.9% | (many diffs) |
| 14 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_024448614.1 | 61.8% | 61.5% | (many diffs) |
| 15 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_011540466.3 | 61.5% | 61.1% | (many diffs) |
| 16 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_011540465.3 | 61.3% | 61% | (many diffs) |
| 17 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_011540464.3 | 61% | 60.7% | (many diffs) |
| 18 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_011540462.3 | 60.5% | 60.2% | (many diffs) |
| 19 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_011540463.2 | 60.5% | 60.2% | (many diffs) |
| 20 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_017000000.1 | 44.9% | 44.5% | (many diffs) |
| 21 | human | 10207 | PATJ | PATJ crumbs cell polarity c... | XM_017000001.1 | 11.8% | 11.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3612
- ORF length:
- 3543
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctgaaaat cctgctacag ataaactgca ggtgctgcag gtacttgatc 121 gcctgaaaat gaaattgcag gagaagggtg acacgtcgca gaatgagaag ttatctatgt 181 tttatgagac actaaagagt cctctcttca accagatact cacacttcag cagtccatca 241 agcaactgaa gggtcaactc aaccatatac cctcagattg ttcagccaac tttgattttt 301 ctaggaaagg tttgttagtg ttcacagatg gttccattac taatggaaat gtccacaggc 361 cctctaataa ctcgactgta tctgggttat ttccgtggac cccgaagttg ggaaatgaag 421 actttaactc agtcattcaa cagatggctc agggccggca aattgaatat atagatatag 481 aacggccttc aactggaggc cttggattca gtgtggtggc cctcagaagt caaaatctcg 541 gaaaagttga tatcttcgtg aaggatgtcc agccagggag tgtagcagac agggatcaaa 601 gattaaagga aaatgatcaa atattggcca ttaatcacac gccattggat cagaacattt 661 cccatcagca agcaattgca ttattacaac aaaccactgg atctttgaga ctgattgtgg 721 ccagggaacc agtccacaca aaaagcagta cttctagcag cctaaatgat acaactctgc 781 ctgaaacagt ttgttggggc catgttgaag aggttgagct cattaatgat ggctctggac 841 taggttttgg aatagttgga ggaaaaacaa gtggcgtggt tgtgaggact atagttcctg 901 gaggattagc agatcgagat ggaagactcc agacagggga ccacatcttg aagattggtg 961 gcacaaacgt gcagggaatg accagtgagc aagttgcaca agttctaagg aactgtggga 1021 attcagtcag gatgctcgtt gctagagatc cagctggtga catttcagtc accccccctg 1081 cccctgcagc cttacctgtt gccctgccta ctgtagccag caagggccct ggttctgaca 1141 gttctctttt tgaaacttat aatgttgagc ttgtgagaaa agatgggcag agtcttggaa 1201 ttagaattgt tggctatgtt ggaacatctc atacagggga agcttcaggg atttatgtga 1261 aaagtgtaat acctggcagt gctgcgtacc acaatggcca cattcaagtg aatgacaaaa 1321 tagttgctgt cgatggcgtg aacattcagg gttttgccaa ccatgatgtt gttgaagtat 1381 tacgaaatgc agggcaggtg gtacacctaa ccctagttcg aaggaagaca tcctcatcta 1441 cttctccact tgaaccacct tcagacagag gaactgttgt agaaccactg aaaccaccag 1501 ctctctttct aactggagca gtggaaactg aaactaatgt ggatggtgaa gatgaggaaa 1561 ttaaagaaag aattgatact ttaaaaaatg acaacataca agccttagaa aaattggaaa 1621 aagtcccaga ctctccagaa aatgagctga aatccagatg ggaaaacctg ttgggtcctg 1681 attatgaagt aatggttgct actttggaca cacagattgc agatgatgct gagttacaga 1741 aatattcaaa gctgctgcct attcacactc tgaggcttgg tgtggaagtg gattcctttg 1801 atgggcacca ttatatttct tcaattgttt ctggtggtcc tgttgataca ttgggtctcc 1861 tacagccaga agatgagctg cttgaggtca atggcatgca gctttatgga aaatctcgcc 1921 gagaagcagt ctcctttctt aaagaagtgc cacccccttt tactttggtt tgctgtcgga 1981 ggttgtttga tgatgaagct tctgtagatg aaccaaggcg cactgaaacc tctcttcctg 2041 agacagaggt tgaccacaat atggatgtca atactgaaga agatgatgat ggggaattag 2101 cactgtggtc ccctgaagtc aagattgttg aactagtaaa agattgtaaa ggtttgggat 2161 tcagcatttt ggattaccag gaccctttag atcctacaag atcagtgatt gtgatccgct 2221 ccctggtagc agatggtgta gcagaaagaa gtgggggact attacctgga gaccgcctgg 2281 tctcagtcaa tgaatactgt ttggacaaca cctcacttgc tgaagctgtg gaaatattga 2341 aagctgtgcc accaggccta gtacaccttg gcatctgtaa gcctttggtg gaagataatg 2401 aagaagaaag ttgttatatt ttacattcaa gcagtaatga agacaagact gaattttcag 2461 gaacaattca tgatataaat tcatctttaa tactcgaagc acccaaggga tttagagatg 2521 aaccatattt taaagaagaa cttgtggatg aaccatttct agatctggga aagtctttcc 2581 attcccaaca aaaagagata gagcaaagca aggaggcctg ggagatgcat gaatttctga 2641 ctcctagatt gcaggaaatg gatgaagaaa gagaaatgct tgttgatgaa gaatatgagt 2701 tatatcaaga tccctcacca tccatggagt tgtatccctt gtcgcacatt caagaggcca 2761 ctcctgtgcc ctctgtgaat gaacttcact ttggtacaca gtggttgcat gataatgaac 2821 catccgagtc tcaagaggca agaaccggga ggactgtcta ttcccaggag gcacagccgt 2881 atggctattg ccctgaaaat gtgatgaaag aaaattttgt catggagtcc ctaccatctg 2941 taccatcaac tgaaggaaac agtcaacaag gcagatttga cgacctggaa aatcttaatt 3001 cattagcaaa aactagtctg gatttaggca tgatcccgaa tgatgtccaa ggtcctagct 3061 tgctcattga ccttcctgtt gtggctcaaa ggagggagca agaagatttg cctttatatc 3121 aacaccaagc gacacgagtt atttccaagg cctcagcata cacaggaatg ttgtcttcta 3181 gatatgccac tgatacatgt gagttacctg agagagaaga aggcgaagga gaagaaactc 3241 caaattttag ccactggggt ccaccgagaa ttgttgagat ttttagagaa cccaatgtgt 3301 ctcttgggat cagtattgtt ggtggacaaa ctgttataaa acgtctaaag aatggagagg 3361 agcttaaagg tatattcatc aaacaagttt tagaagacag tccagcaggg aagacgaacg 3421 cacttaaaac tggagataaa atacttgagg tgtctggagt agatttgcag aatgcctcac 3481 acagcgaagc agttgaggcc attaagaatg caggaaaccc tgtggtgttc attgttcaga 3541 gtttgtcatc cactccagcc tgggtgacag agcaagactc tgtctcgaaa aagaaaaaaa 3601 accatgaagg attgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 3661 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 3721 caatcaacct ctggatta