Construct: ORF ccsbBroad304_11563

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008318.1_s304c1, BRDN0000411422
Derived from:
ccsbBroadEn_11563
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CFHR3 (10878)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11563
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10878 CFHR3 complement factor H related 3 XR_241062.3 71.6% 1_71del;768_972del
2 human 10878 CFHR3 complement factor H related 3 NM_021023.5 67.1% 61.2% (many diffs)
3 human 10878 CFHR3 complement factor H related 3 NM_001166624.1 66.6% 62.1% (many diffs)
4 human 10878 CFHR3 complement factor H related 3 XR_426757.2 63.2% 1_71del;768_1101del
5 human 10877 CFHR4 complement factor H related 4 XM_017000112.1 44.3% 33.7% (many diffs)
6 human 10877 CFHR4 complement factor H related 4 NM_006684.5 40.7% 34.3% (many diffs)
7 human 10877 CFHR4 complement factor H related 4 XM_017000111.1 40.2% 30.4% (many diffs)
8 human 10877 CFHR4 complement factor H related 4 XM_017000110.1 39.6% 29.4% (many diffs)
9 human 10878 CFHR3 complement factor H related 3 XR_001736938.1 38.8% 1_71del;768_1791del
10 human 10878 CFHR3 complement factor H related 3 XR_002958987.1 38.5% 1_71del;768_1805del
11 human 10877 CFHR4 complement factor H related 4 NM_001201551.2 36.2% 27.3% (many diffs)
12 human 10877 CFHR4 complement factor H related 4 NM_001201550.3 36.1% 27.3% (many diffs)
13 human 10878 CFHR3 complement factor H related 3 XR_001736937.1 31.6% 1_71del;768_2198del
14 human 3075 CFH complement factor H NM_001014975.2 25.7% 21.3% (many diffs)
15 human 3075 CFH complement factor H XM_017001108.2 25.5% 21.3% (many diffs)
16 human 3075 CFH complement factor H NM_000186.4 16.5% 12.9% (many diffs)
17 human 3075 CFH complement factor H XR_001737134.2 14.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
762
ORF length:
696
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt gttactaatc aatgtcattc tgaccttgtg ggtttcctgt gctaatggac
121aagtgaaacc ttgtgatttt ccagacatta aacatggagg tctatttcat gagaatatgc
181gtagaccata ctttccagta gctgtaggaa aatattactc ctattactgt gatgaacatt
241ttgagactcc ttcaggaagt tactgggatt acattcattg cacacaaaat gggtggtcac
301cagcagtacc atgtctcaga aaatgttatt ttccttattt ggaaaatgga tataatcaaa
361attatggaag aaagtttgta cagggtaact ctacagaagt tgcctgccat cctggctacg
421gtcttccaaa agcgcagacc acagttacat gtacggagaa aggctggtct cctactccca
481gatgcatccg tgtcagaaca tgctcaaaat cagatataga aattgaaaat ggattcattt
541ccgaatcttc ctctatttat attttaaata aagaaataca atataaatgt aaaccaggat
601atgcaacagc agatggaaat tcttcaggat caattacatg tttgcaaaat ggatggtcag
661cacaaccaat ttgcattact gcttgtattg cattccgtgc tcacgctcag aaaagttgta
721catgtcgggg gagaaatgag tgcttaattc tgaatttctg ctacccaact ttcttgtaca
781aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
841ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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