Construct: ORF ccsbBroad304_11708
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000576.1_s304c1, BRDN0000404932
- Derived from:
- ccsbBroadEn_11708
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCF2L (23263)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11708
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | NM_001320817.2 | 100% | 100% | |
| 2 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_017020499.2 | 93.6% | 92.5% | (many diffs) |
| 3 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | NM_024979.4 | 87.6% | 87.4% | 2947_2951delGGTTG;2956_2962delAAAACGT;2965_3369del |
| 4 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | NM_001112732.3 | 86.4% | 84.5% | (many diffs) |
| 5 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | NM_001320816.2 | 86% | 84.9% | (many diffs) |
| 6 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | NM_001320815.2 | 85.6% | 85.5% | 2948_2958del;2961T>C;2964_3444del |
| 7 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | NM_001366644.2 | 85.2% | 85% | (many diffs) |
| 8 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_011537487.1 | 83.4% | 83.2% | 2948_2961del;2965T>C;2967_3537del |
| 9 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_017020495.1 | 82.6% | 81.4% | (many diffs) |
| 10 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_011537485.1 | 82.3% | 80.6% | (many diffs) |
| 11 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_011537484.1 | 81.9% | 80.9% | (many diffs) |
| 12 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_017020494.1 | 81.8% | 81.1% | (many diffs) |
| 13 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | NM_001366646.2 | 81.8% | 79.2% | (many diffs) |
| 14 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_011537488.1 | 81.5% | 81.2% | (many diffs) |
| 15 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_011537490.1 | 81.1% | 81% | (many diffs) |
| 16 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_017020493.1 | 80.8% | 79.7% | (many diffs) |
| 17 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_011537482.1 | 80.1% | 79% | (many diffs) |
| 18 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_011537483.2 | 80.1% | 79% | (many diffs) |
| 19 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | NM_001366645.2 | 80% | 77.5% | (many diffs) |
| 20 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_011537486.2 | 78.8% | 77.7% | (many diffs) |
| 21 | human | 23263 | MCF2L | MCF.2 cell line derived tra... | XM_011537491.1 | 77.9% | 75.5% | (many diffs) |
| 22 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508723.3 | 81% | 79.7% | (many diffs) |
| 23 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508721.3 | 78.7% | 81.5% | (many diffs) |
| 24 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | NM_001159485.1 | 74.4% | 77.3% | (many diffs) |
| 25 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | NM_001159486.1 | 74.1% | 77% | (many diffs) |
| 26 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508720.3 | 74% | 76.3% | (many diffs) |
| 27 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508719.3 | 72.9% | 74% | (many diffs) |
| 28 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508718.3 | 72.8% | 73.9% | (many diffs) |
| 29 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_017312595.1 | 72.4% | 74.6% | (many diffs) |
| 30 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508715.2 | 71.6% | 74.4% | (many diffs) |
| 31 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508716.3 | 71.3% | 73.7% | (many diffs) |
| 32 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508714.3 | 71.3% | 72.3% | (many diffs) |
| 33 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508717.3 | 71.1% | 73.3% | (many diffs) |
| 34 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508712.3 | 71% | 72.3% | (many diffs) |
| 35 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508711.3 | 70.9% | 73.4% | (many diffs) |
| 36 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | NM_178076.3 | 70.9% | 73.1% | (many diffs) |
| 37 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508710.3 | 70.7% | 73.2% | (many diffs) |
| 38 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508713.3 | 70.5% | 72.7% | (many diffs) |
| 39 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508709.3 | 69.6% | 70.7% | (many diffs) |
| 40 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508708.3 | 69.6% | 70.6% | (many diffs) |
| 41 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508706.3 | 69.4% | 70.5% | (many diffs) |
| 42 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508707.2 | 69.4% | 70.5% | (many diffs) |
| 43 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_011242040.1 | 69.4% | 70.5% | (many diffs) |
| 44 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_017312593.1 | 69.4% | 70.5% | (many diffs) |
| 45 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XM_006508722.2 | 60.1% | 62.2% | (many diffs) |
| 46 | mouse | 17207 | Mcf2l | mcf.2 transforming sequence... | XR_001778409.1 | 31.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3018
- ORF length:
- 2952
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac ggtgcgccgg ctgtcactgc tgtgccggga cctctgggcg ctgtggctgc 121 tgctgaaggc cggcgcagat gaaatcatgc accaggacat cgtcccgctc tgtgctgccg 181 acatccagga ccagctaaag aagcgctttg cttacctgtc cggtgggcgg gggcaggacg 241 gaagcccggt tatcaccttc cctgactacc cggccttcag cgagattccg gacaaggagt 301 tccagaatgt catgacctac ctcaccagca tccccagcct gcaggacgct ggcatcggat 361 tcatcctggt gatagaccgg cgacgggaca aatggacctc cgtgaaggcg tccgtcctgc 421 gcatcgcagc atctttcccg gcaaacctgc agctcgtcct cgtgcttcgc ccgacgggtt 481 ttttccaaag gactctctcc gacatcgctt tcaaattcaa tagagatgac tttaagatga 541 aggtgccggt cataatgctg agctccgtac cagacttaca cggttacatc gataagtcgc 601 agctgaccga ggacctgggt gggaccctgg actactgcca ctcccggtgg ctgtgccagc 661 gcacggccat cgaaagtttc gccctcatgg tgaagcagac ggctcagatg ctgcagtcct 721 tcgggaccga gctggctgaa acagagctgc ccaatgacgt ccagtcgaca agctcagtgc 781 tgtgtgcgca cacagagaag aaggacaagg cgaaggagga tttgaggctg gcactgaaag 841 aggggcacag tgtcctggag agcctcaggg agctgcaggc tgagggctca gagcccagtg 901 tgaaccagga ccagcttgac aaccaggcca ccgtgcagag gctcctggcc cagctgaacg 961 aaaccgaggc tgccttcgat gagttctggg caaagcatca gcagaaactg gagcagtgtc 1021 tgcagctccg gcactttgag cagggcttcc gggaggtcaa agccatcttg gacgcagcgt 1081 cccagaagat agcaaccttc acagacatcg gcaacagcct ggcgcatgtg gagcacctgc 1141 tgagggacct ggccagcttc gaggagaaat caggcgtggc cgtggagagg gcccgggccc 1201 tgtctctgga cggcgagcag ctcattggga acaagcacta cgcggtagac tccatccgcc 1261 caaagtgcca ggagctccgg cacctctgtg accagttctc tgcggagatc gcaaggagga 1321 gggggctgct cagcaagtcc ctggagctgc accgccgcct ggagacgtcc atgaagtggt 1381 gtgatgaagg gatttacctg ctggcctcac aacctgtgga caagtgccag tcccaggacg 1441 gcgcggaggc tgccctccag gaaatcgaga agtttttgga gaccggtgcg gaaaataaga 1501 tccaggagct caacgcgatt tacaaggaat acgaatccat cctcaaccaa gatctcatgg 1561 agcacgtgcg aaaggtcttc cagaagcagg caagcatgga ggaggtgttc caccgcaggc 1621 aggccagcct gaagaagctg gcggccaggc agacgcggcc cgtgcagccg gtggccccca 1681 gacccgaggc actggcaaag tcgccctgcc cctccccagg cattcggcga ggctctgaga 1741 actccagctc cgagggcggt gcgctccgga gagggcccta ccggagggcc aagagtgaga 1801 tgagtgagag ccggcagggc cgcggctcag cgggggagga ggaggaaagc ctggccatcc 1861 tgcgcaggca cgtgatgagc gagctcctgg acacagaacg ggcctacgtg gaggagctgc 1921 tgtgcgtcct ggagggctac gccgcggaga tggataaccc actgatggct cacctcctgt 1981 caacaggcct tcacaacaag aaggatgttt tgtttggaaa catggaggaa atctatcact 2041 tccacaacag gatattcctc agggagctgg aaaactacac tgactgccca gaactggttg 2101 gaagatgctt tctggagagg atggaagatt tccagatcta tgagaagtac tgtcagaaca 2161 agccccgctc tgagagcctg tggagacagt gctccgactg cccgtttttc caggaatgcc 2221 agagaaagct ggaccacaag ctgagcctgg actcctacct gctgaagcca gtgcagagga 2281 tcaccaagta ccagctgctg ctcaaggaaa tgctgaaata cagcaggaac tgcgaggggg 2341 ctgaggacct gcaggaggcg ctgagctcca tcctgggcat cctgaaggcc gtgaacgact 2401 ccatgcacct catcgctatc accggctatg acgggaatct cggcgacctg ggcaagctgc 2461 tgatgcaggg ctcgttcagc gtctggaccg accacaagag gggccacacc aaggtgaagg 2521 agctggccag gttcaagccc atgcagcggc acctgttcct gcacgagaag gcagtgctct 2581 tctgcaagaa gagggaggag aatggggagg ggtatgagaa agctccctcc tacagctaca 2641 agcagtcctt aaacatggct gccgttggca ttacggagaa cgtgaaggga gatgctaaga 2701 agttcgagat ctggtacaac gcgcgcgagg aggtctacat cgtccaggcg ccaactcctg 2761 agattaaagc cgcgtgggtg aatgaaattc ggaaagtgct gaccagccag ctgcaggctt 2821 gtagagaagc cagccagcac cgggcgctgg agcagtcaca gagcctgccc ctgccggccc 2881 cgaccagcac cagtccctca agaggaaact caaggaacat caagaagctg gaagaaagga 2941 aaacagaccc cctaagcctg gagggatacg tcagctcagc gccactgaca aagccccccg 3001 aaaagggcaa agagccctac ccaactttct tgtacaaagt ggttggtaag cctatcccta 3061 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 3121 agtcgacaat caacctctgg atta