Transcript: Mouse NM_178076.3

Mus musculus mcf.2 transforming sequence-like (Mcf2l), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Mcf2l (17207)
Length:
5269
CDS:
84..3611

Additional Resources:

NCBI RefSeq record:
NM_178076.3
NBCI Gene record:
Mcf2l (17207)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_178076.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000110010 GCACGTTGTTTATACAACTAA pLKO.1 4411 3UTR 100% 0.563 0.450 N Mcf2l n/a
2 TRCN0000110014 GTTTGGAAACATGGAGGAAAT pLKO.1 2039 CDS 100% 10.800 7.560 N Mcf2l n/a
3 TRCN0000110011 CCAGAACTACACGGCTACATT pLKO.1 594 CDS 100% 5.625 3.938 N Mcf2l n/a
4 TRCN0000110012 GCGGTTTCCAAGAACACTGAT pLKO.1 144 CDS 100% 4.950 3.465 N Mcf2l n/a
5 TRCN0000110013 GCTGATTGAGAACAGGCACTA pLKO.1 1244 CDS 100% 4.050 2.835 N Mcf2l n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178076.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11708 pDONR223 100% 70.9% 73.1% None (many diffs) n/a
2 ccsbBroad304_11708 pLX_304 0% 70.9% 73.1% V5 (many diffs) n/a
3 TRCN0000467665 TTAGCTTTGATTCAATATCGGCAC pLX_317 12.1% 70.9% 73.1% V5 (many diffs) n/a
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