Construct: ORF ccsbBroad304_11713

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005457.1_s304c1, BRDN0000404646
Derived from:
ccsbBroadEn_11713
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMG6 (23293)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11713
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23293 SMG6 SMG6 nonsense mediated mRNA... NM_001256827.2 100% 100%
2 human 23293 SMG6 SMG6 nonsense mediated mRNA... NM_001256828.1 100% 100%
3 human 23293 SMG6 SMG6 nonsense mediated mRNA... XM_005256571.5 100% 100%
4 human 23293 SMG6 SMG6 nonsense mediated mRNA... XM_011523775.2 100% 100%
5 human 23293 SMG6 SMG6 nonsense mediated mRNA... XM_017024399.2 100% 100%
6 human 23293 SMG6 SMG6 nonsense mediated mRNA... NM_001282326.1 62.1% 59.6% (many diffs)
7 human 23293 SMG6 SMG6 nonsense mediated mRNA... XM_005256569.4 36.8% 36.8% 1_2631del
8 human 23293 SMG6 SMG6 nonsense mediated mRNA... XM_011523769.2 36.8% 36.8% 1_2631del
9 human 23293 SMG6 SMG6 nonsense mediated mRNA... XM_024450681.1 36.8% 36.8% 1_2631del
10 human 23293 SMG6 SMG6 nonsense mediated mRNA... NM_017575.5 36% 36% 1_2724del
11 mouse 103677 Smg6 Smg-6 homolog, nonsense med... XM_011248660.1 92.6% 97.6% (many diffs)
12 mouse 103677 Smg6 Smg-6 homolog, nonsense med... XM_011248659.2 33.6% 35.5% (many diffs)
13 mouse 103677 Smg6 Smg-6 homolog, nonsense med... NM_001002764.1 33.3% 35.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1599
ORF length:
1533
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gacattccct gcagtggctg agaaggtcct caaggagttc caggtgttac
121tgcagcacag cccctctccc attggaagta cccgcatgct gcagcttatg accatcaata
181tgtttgcagt acacaactcc cagctgaaag actgcttctc ggaggagtgc cgctctgtga
241tccaggaaca agccgcagct ctgggcttgg ccatgttttc tctactggtc cgccgctgca
301cctgcttact taaggagtcc gccaaagctc agctgtcctc tcctgaggac caggatgacc
361aagacgacat caaggtgtct tcctttgtcc cggacctgaa ggagctgctc cccagtgtca
421aagtctggtc agattggatg ctcggctacc cggacacctg gaatcctcct cccacatccc
481tggatctgcc ctcgcatgtt gctgtggatg tatggtcgac gctggctgat ttctgtaaca
541tactgactgc agtgaatcag tctgaggtgc cactgtacaa ggacccggat gatgacctca
601cccttcttat cctggaagag gatcggcttc tctcgggctt tgtccccttg ctggctgccc
661ctcaggaccc ctgctacgtg gagaaaacct cggataaggt tattgcagct gactgcaaaa
721gggtcacagt gctgaagtat tttctggaag ccctttgtgg acaagaagag cctctgctgg
781cattcaaggg tggaaagtat gtgtcagtgg cacccgtccc agacaccatg ggaaaggaaa
841tgggaagcca agagggaaca cgactggaag atgaggagga ggatgtggtg attgaagact
901ttgaggaaga ttcagaggct gaaggcagcg gaggcgagga tgacatcagg gagcttcggg
961ccaagaagct ggctctggcc aggaagatag ctgagcagca gcgtcgccag gaaaagatcc
1021aggctgtcct ggaggaccac agtcagatga ggcagatgga gctcgaaatc agacctttgt
1081tcctcgtacc agacaccaac ggcttcattg accacctggc cagtctggcg cggctgctgg
1141agagcaggaa gtacatcctg gtggtgcccc tcatcgtgat caatgagctg gacggcctgg
1201ccaaggggca ggagacagac caccgggctg ggggctacgc ccgtgtggta caagagaagg
1261cccgcaagtc catcgagttc ctcgagcagc gattcgagag tcgggactct tgcctgcgag
1321ccctgaccag ccgtggcaat gaactcgaat ccatcgcctt ccgcagtgag gacatcactg
1381gccagctggg taacaacgat gatctcatcc tgtcctgctg cctccactac tgcaaagaca
1441aggctaagga cttcatgccc gccagcaaag aggagccaat ccggctactg cgggaggtgg
1501tgctgttgac ggatgaccgg aacctgcgtg tgaaggcgct cacaaggaat gttcctgtac
1561gggacatccc agccttcctc acgtgggccc aggtgggctg cccaactttc ttgtacaaag
1621tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1681gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)