Construct: ORF ccsbBroad304_11749
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009545.1_s304c1, BRDN0000399246
- Derived from:
- ccsbBroadEn_11749
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSD4 (23550)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11749
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23550 | PSD4 | pleckstrin and Sec7 domain ... | XM_006712392.2 | 97.2% | 97.1% | (many diffs) |
| 2 | human | 23550 | PSD4 | pleckstrin and Sec7 domain ... | NM_012455.3 | 97.1% | 97% | (many diffs) |
| 3 | human | 23550 | PSD4 | pleckstrin and Sec7 domain ... | XM_005263634.2 | 97.1% | 97% | (many diffs) |
| 4 | human | 23550 | PSD4 | pleckstrin and Sec7 domain ... | XM_006712394.2 | 88.6% | 77.8% | (many diffs) |
| 5 | human | 23550 | PSD4 | pleckstrin and Sec7 domain ... | XM_006712393.2 | 88.5% | 77.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3147
- ORF length:
- 3081
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat gggtgactac agactccctg accaccccca gcccatggaa attctcaacc 121 tgtacttggg agacagcctg gagccccacc caggagagtg cccaagggaa acgtgcagcc 181 atgaggatcc accggagcct ttcgaggagc aaacctgggc cactgaccct cctgaaccta 241 ccagacaaaa tgttcctccc tggggctccg gtgtggagct cacacacctg gggagctggg 301 tccatcagga cgggctggag ccttgccagg agcaaacccg ggccactgac cctcctgaat 361 ctaccagaca agatgctcct ccctggggct ccggtgtgga gctcacacac ctggggagcc 421 cctctgccca gagggatcac aggcagaaca cagcatcacc agggtcacca gtgaacagcc 481 atctaccggg gagcccaaag cagaaccgga gcacgtccac acaggtagtg ttctgggcag 541 gcatcctgca ggcccagatg tgtgtcctag acctggagga ggagctggag aagacggaag 601 ggctcaaggc tgggctgaaa tgctgtctcc ccacgccccc tgtggacctc cccggggaca 661 cgggcctgca ctccagccca cctgagaatg aagactcagg ggaagacagc agcgagcctg 721 agggagaggg ccaggcatgg ctgagagagg gaaccccaga ctcttcccca cagtggggag 781 ctgaggagga gagcatgttc ttcagcaacc ccctcttcct ggcgagtcct tgctcagaga 841 acagtgcttc tggagagtgc ttttcctggg cggcttcaga ctcccatgca ggtgtgagga 901 ctggacctga gagcccagcg actctggagc ctcccctccc agaagacaca gtgctgtggg 961 agctggaaag tgagccagat ttgggggacg gcgctgctat cagtgggcat tgtacccctc 1021 cattccctgt gcccatctat aaaccacact ccatctgctg ggcctcagtg gctgccgctg 1081 agggggctcc tgcagcacct cctggtcacg gggagagtga gggagatagg cttggtcctg 1141 ctccatctgc agcaccgtgt gtggacgaag cattgacctg ggaatcagga tgtgtcggat 1201 ctgatcttgg ccctgctgca catcctgtgc aaccttgggc ctctctcagc cctgagggct 1261 ggcagagagg aggtcctttt tggccccagg tgactcttaa ctcccaggac agagagccta 1321 gcagcccaga atctgagagc agaggccctg gtcccaggcc cagccctgca tcgtcccagg 1381 agggcagccc gcagcttcaa caccacagct caggcatttt gcccaagtgg acactagatg 1441 cttcacagtc ttcactcttg gagacggatg gggaacagcc aagttccttg aagaaaaagg 1501 aggcagggga ggccccaaaa ccaggcgagg aagtaaagag tgaaggaaca gccaggcctg 1561 cagagactgg agacgtccag cctgacattc acctgacttc tgcagaacat gagaatctga 1621 ggacaccgat gaactcttct tggcttcctg ggagccctat gccccaagca cagtccccag 1681 aggaaggcca gagaccacca gctggagaca agctagctaa tggcgtcagg aacaacaagg 1741 tagcctggaa cttggcctca cgcctctatc gcctggaggg cttccggaag tctgaagtgg 1801 ctgcctacct gcagaagaac aatgacttta gcagggctgt ggctgaggag tacctgtcct 1861 tcttccagtt tggaggccag agtctggacc gagccctccg gagcttcctc caggccttgg 1921 tgctcagtgg ggagactcag gaacgggagc gaatcctcta ccagttctcc agacgcttcc 1981 accattgcaa tccggggatc ttcccctcag tagattctgt acacaccttg acatgtgcaa 2041 tcatgctgct taacacggac ctgcatggac agaacattgg gaagagcatg agctgccagg 2101 aattcataac caacctgaat gggctgaggg atggcgggaa cttccccaag gagctgctga 2161 aggccctcta ctggtctatc cgcagcgaga agctcgagtg ggccgtggat gaagaagaca 2221 cagccagacc tgagaaggcc cagccgtccc tgccagctgg caagatgagc aagcccttcc 2281 ttcagctggc tcaggatccc acagtgccca cctacaagca gggcatcctg gctcggaaaa 2341 tgcatcaaga tgcagacggc aagaagacgc catggggcaa gcgtggctgg aagatgttcc 2401 acaccttact gcgagggatg gttctctact tcctgaaggg agaagaccac tgtctggagg 2461 gggagagctt ggtggggcag atggtggatg agcccgtggg ggtgcaccac tcgctggcca 2521 cccccgccac gcattacacc aagaagccgc acgtcttcca gctgcgcacg gctgactggc 2581 gcctctacct cttccaggca cccactgcca aggagatgag ctcctggatc gcgcgcatca 2641 acttggctgc ggccacccac tccgcgccgc ccttccccgc cgctgtgggc tcccagcgca 2701 gattcgtgcg gcccatcctg cccgtgggcc ccgcccagag ctccctggag gagcagcatc 2761 gatcccacga gaactgcctg gacgctgccg cggacgacct gctggatcta cagaggaacc 2821 tgccggagcg gcggggccgt ggccgcgagc tggaggagca ccgcctgcgg aaggagtacc 2881 tggagtacga gaaaacccgc tacgagacct acgtgcagct gctggtggcc cgcctgcact 2941 gcccctctga tgctctggac ctgtgggagg agcagctggg gagggaagct ggaggcactc 3001 gggagcccaa gctcagcctg aagaagtccc actcgagccc gtccctgcac caggatgagg 3061 ctcccaccac ggccaaggtg aagcgcaaca tctcagagcg cagaacctac cggaagatca 3121 tccctaagcg gaaccgcaat cagctgtgcc caactttctt gtacaaagtg gttggtaagc 3181 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 3241 ggcgcgttaa gtcgacaatc aacctctgga tta