Construct: ORF ccsbBroad304_11845

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004867.1_s304c1, BRDN0000405305
Derived from:
ccsbBroadEn_11845
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMC1B (27127)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11845
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27127 SMC1B structural maintenance of c... NM_001291501.2 99.9% 99.9% 3148C>A
2 human 27127 SMC1B structural maintenance of c... XM_011530145.2 94.3% 93.7% (many diffs)
3 human 27127 SMC1B structural maintenance of c... NM_148674.5 93.9% 93.9% 3148C>A;3272_3493del
4 human 27127 SMC1B structural maintenance of c... XM_011530144.2 90.6% 90.6% 0_1ins123;3025C>A;3149_3370del
5 human 27127 SMC1B structural maintenance of c... XR_244368.4 82.2% (many diffs)
6 mouse 140557 Smc1b structural maintenance of c... NM_080470.1 79.5% 76.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3552
ORF length:
3483
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcccacctg gagctgctgc ttgtggaaaa tttcaagtcg tggcggggcc
121gccaggtcat tggccccttc cggaggttca cctgcatcat cggccccaac ggctctggaa
181aatctaatgt aatggatgca cttagttttg taatgggaga gaaaatagct aatttaagag
241tgaaaaatat tcaagaactc attcatggag cacatattgg aaaacctatt tcttcttctg
301caagtgtaaa aattatatat gtggaggaaa gtggcgaaga gaaaacattt gcaaggatta
361tccgaggggg atgctcagaa tttcgcttta atgataatct tgtgagtcgt tctgtttaca
421ttgcagagtt ggaaaagata ggcataatag tcaaagcaca aaattgtttg gtttttcagg
481gaactgtaga gtcaatttca gtgaagaaac ccaaagaaag gacccagttt tttgaggaaa
541tcagcacttc aggagagctt ataggagaat atgaagaaaa gaaaagaaag ttacaaaaag
601ccgaagagga tgcacagttt aactttaata agaaaaaaaa tatagcggca gagcgcagac
661aagcaaaatt agagaaggaa gaggcagaac gttaccagag tctccttgaa gaactgaaaa
721tgaacaagat acaactgcag ctttttcaac tataccataa tgagaaaaag attcatctcc
781tgaacaccaa gttagagcat gtgaataggg atttgagtgt caaaagagag tctttgtctc
841atcatgaaaa catagttaaa gccaggaaaa aggaacatgg aatgctaact agacaactac
901aacaaacaga aaaagaatta aaatcggttg aaaccctttt aaatcagaag aggcctcagt
961acattaaagc caaagaaaac acttctcacc accttaagaa attagatgtg gctaagaaat
1021caataaagga cagcgaaaaa caatgttcta aacaggaaga tgatataaaa gccctggaga
1081cagagctggc tgatttagat gctgcatgga gaagttttga aaagcagatt gaggaagaaa
1141ttttacataa aaagcgagac attgaactgg aagccagtca gctggatcgt tataaagaac
1201ttaaggaaca agtaagaaag aaagtagcta caatgactca acaactggaa aaactgcagt
1261gggaacagaa gacagatgaa gaaagactgg catttgaaaa gaggaggcat ggagaagttc
1321agggaaatct aaaacaaata aaagaacaaa tagaagatca taaaaaacga atagagaagt
1381tagaggagta tacaaagaca tgcatggatt gcttgaaaga gaaaaaacag caagaggaaa
1441ccctagtgga tgaaattgaa aaaacaaaat caagaatgtc tgaagttaat gaagaattga
1501atcttattag aagtgaattg cagaatgctg ggattgatac ccatgaggga aaacgtcagc
1561aaaagagagc agaggttctg gaacacctta aaagactgta cccagattct gtgtttggaa
1621gactatttga cctgtgtcat cctattcata agaaatacca gctggctgtt actaaggttt
1681ttggccggtt catcactgcc attgttgtag cctctgaaaa ggtagcaaaa gattgtattc
1741gatttctgaa ggaggaaaga gctgaacctg agacattcct cgctctagat taccttgata
1801tcaagccaat caatgaaaga ctaagggagc ttaaaggctg taaaatggtg attgatgtca
1861taaagactca gtttcctcag ctgaagaaag tgattcagtt tgtgtgtgga aatggtcttg
1921tttgtgagac tatggaagaa gcaaggcata ttgcactcag tggacctgaa agacagaaaa
1981cagtagctct tgatggaaca ttatttttaa aatctggagt gatctctgga gggtcaagtg
2041acttaaaata caaggctaga tgctgggatg agaaagagtt aaagaatcta agagacagac
2101gaagccagaa aatccaagag ctaaagggtt taatgaagac actccgcaaa gaaacagatt
2161tgaaacaaat acagaccctg atacagggaa ctcaaacacg actcaaatat tcacaaaatg
2221aactagagat gattaagaag aagcaccttg ttgcttttta ccaggaacaa tctcagttac
2281aaagtgaact actaaatatt gagtctcaat gtattatgtt gagtgaagga atcaaggaac
2341gacaacgaag aattaaagaa tttcaagaaa agatagataa ggtagaagac gatatcttcc
2401aacacttctg tgaagaaatt ggcgtggaaa atattcgtga atttgagaac aaacatgtta
2461aacggcaaca agaaattgat caaaaaagat tagaatttga aaaacaaaaa actcggctta
2521atgttcaact tgagtatagt cgcagtcacc ttaagaagaa actgaataag atcaacacat
2581taaaagaaac tatccagaaa ggtagtgaag atattgatca cctaaagaag gctgaagaaa
2641actgtctgca gacagtgaat gaactcatgg caaagcagca gcaacttaag gacatacgtg
2701tcactcagaa ctccagtgcc gagaaagttc aaactcaaat tgaagaggaa cggaagaagt
2761ttctggctgt tgatagggaa gtggggaaat tgcaaaaaga agttgtaagt attcaaactt
2821ctctggaaca gaaacgatta gagaagcata acttgctgct tgattgcaaa gtgcaagaca
2881ttgagataat ccttttgtcg gggtcactgg atgacatcat tgaagtggag atgggaactg
2941aagcagaaag tacccaggca acaattgata tctatgaaaa agaagaagcc tttgaaatag
3001actacagctc tctaaaagag gatttgaagg ctctacagtc tgatcaagaa atcgaggccc
3061accttaggct cttattgcag caagtagcat cccaggaaga tatcttactg aaaacagcag
3121ccccaaacct acgagcactg gagaacttaa agactgtcag agacaagttt caagagtcca
3181cagatgcttt tgaggccagc agaaaggaag ccagaatgtg taggcaagag ttcgagcaag
3241tgaaaaaaag gagatacgat cttttcaccc agtgttttga gcatgtctca atctcaattg
3301atcaaatcta caagaagctc tgcagaaaca acagcgccca agtgtcaagt tacatcaaag
3361agcaaactca agaccagttt cagatgatag tcatctccct aaaagaagag ttctattcca
3421gagccgacgc gctgatcggc atctatcctg agtacgatga ctgcatgttc agccgagttt
3481tgaccctaga tctttctcag tatccagaca ctgaaggcca agaaagcagc aagagacacg
3541gagagtcccg cttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
3601tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
3661caatcaacct ctggatta

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