Construct: ORF ccsbBroad304_11902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014166.1_s304c1, BRDN0000400098
- Derived from:
- ccsbBroadEn_11902
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP25 (29761)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11902
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29761 | USP25 | ubiquitin specific peptidas... | XM_011529535.2 | 64.2% | 53.1% | (many diffs) |
| 2 | human | 29761 | USP25 | ubiquitin specific peptidas... | XM_017028325.1 | 47.6% | 38.2% | (many diffs) |
| 3 | human | 29761 | USP25 | ubiquitin specific peptidas... | NM_013396.6 | 42.6% | 42.6% | 779_2593del |
| 4 | human | 29761 | USP25 | ubiquitin specific peptidas... | NM_001283042.3 | 41.3% | 41.3% | 779_2689del |
| 5 | human | 29761 | USP25 | ubiquitin specific peptidas... | XM_011529529.1 | 40% | 32% | (many diffs) |
| 6 | human | 29761 | USP25 | ubiquitin specific peptidas... | NM_001283041.2 | 40% | 40% | 779_2803del |
| 7 | human | 29761 | USP25 | ubiquitin specific peptidas... | XR_001754834.1 | 26.6% | 1_447del;1226_2897del;3470_5059del | |
| 8 | human | 29761 | USP25 | ubiquitin specific peptidas... | XR_002958593.1 | 25.8% | 1_447del;1226_3061del;3634_5220del | |
| 9 | human | 29761 | USP25 | ubiquitin specific peptidas... | XR_002958592.1 | 25.4% | 1_447del;1228_3153del;3724_5313del | |
| 10 | mouse | 30940 | Usp25 | ubiquitin specific peptidas... | NM_013918.2 | 37.8% | 40.7% | (many diffs) |
| 11 | mouse | 30940 | Usp25 | ubiquitin specific peptidas... | XM_006523056.2 | 35.5% | 38.2% | (many diffs) |
| 12 | mouse | 30940 | Usp25 | ubiquitin specific peptidas... | XM_006523055.2 | 35.2% | 37.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1416
- ORF length:
- 1350
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac cgtggagcag aacgtgctgc agcagagcgc ggcgcagaag caccagcaga 121 cgtttttgaa tcaactgaga gaaattacgg ggattaatga cacccagata ctacagcaag 181 ccttgaagga tagtaatgga aacttggaat tagcagtggc tttccttact gcgaagaatg 241 ctaagacccc tcagcaggag gagacaactt actaccaaac agcacttcct ggcaatgata 301 gatacatcag tgtgggaagc caagcagata caaatgtgat tgatctcact ggagatgata 361 aagatgatct tcagagagca attgccttga gtttggccga atcaaacagg gcattcaggg 421 agactggaat aactgatgag gaacaagcca ttagcagagt tcttgaagcc agcatagcag 481 agaataaagc atgtttgaag aggacaccta cagaagtttg gagggattct cgaaaccctt 541 atgatagaaa aagacaggac aaagctcccg ttgggctaaa gaatgttggc aatacttgtt 601 ggtttagtgc tgttattcag tcattattta atcttttgga atttagaaga ttagttctga 661 attacaagcc tccatcaaat gctcaagatt taccccgaaa ccaaaaggaa catcggaatt 721 tgccttttat gcgtgagctg aggtatctat ttgcacttct tgttggtacc aaaaggaagt 781 atgttgatcc atcaagagca gttgaaattc ttaaggatgc tttcaaatca aatgactcac 841 agcaggagtg gcatcaggat tataggaaat tcagggaaac aactatgtat ctcataattg 901 ggctagaaaa ttttcaaaga gaaagttata tagattcctt gctgttcctc atctgtgctt 961 atcagaataa caaagaactc ttgtctaaag gcttatacag aggacatgat gaagaattga 1021 tatcacatta tagaagagaa tgtttgctaa aattaaatga gcaagccgca gaactcttcg 1081 aatctggaga ggatcgagaa gtaaacaatg gtttgattat catgaatgag tttattgtcc 1141 catttttgcc attattactg gtggatgaaa tggaagaaaa ggatatacta gctgtagaag 1201 atatgagaaa tcgatggtgt tcctaccttg gtcaagaaat ggaaccacac ctccaagaaa 1261 agctgacaga ttttttgcca aaactgcttg attgttctat ggagattaaa agtttccatg 1321 agccaccgaa gttaccttca tattccacgc atgaactctg tgagcgattt gcccgaatca 1381 tgttgtccct cagtcgaact cctgctgatg gaagataccc aactttcttg tacaaagtgg 1441 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1501 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta