Construct: ORF ccsbBroad304_11913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004419.1_s304c1, BRDN0000406074
- Derived from:
- ccsbBroadEn_11913
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX12 (29934)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11913
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29934 | SNX12 | sorting nexin 12 | NM_001256185.1 | 93.6% | 92.4% | 478_478delGinsAGTCTTGCTGTGTCT;482delG;486_487insGCTGGAGTGCAGTGGCA |
| 2 | human | 29934 | SNX12 | sorting nexin 12 | NM_013346.4 | 93.6% | 92.4% | 478_478delGinsAGTCTTGCTGTGTCT;482delG;486_487insGCTGGAGTGCAGTGGCA |
| 3 | human | 29934 | SNX12 | sorting nexin 12 | NM_001256188.1 | 91.2% | 90.1% | (many diffs) |
| 4 | human | 29934 | SNX12 | sorting nexin 12 | NM_001256187.1 | 55.8% | 51.1% | 261_262ins125;288_289ins103 |
| 5 | human | 29934 | SNX12 | sorting nexin 12 | NM_001256186.1 | 53.4% | 48.8% | 152_153insGGTTCGCATGCG;249_250ins125;276_277ins103 |
| 6 | mouse | 55988 | Snx12 | sorting nexin 12 | NM_001110311.1 | 90.9% | 93% | (many diffs) |
| 7 | mouse | 55988 | Snx12 | sorting nexin 12 | NM_018875.2 | 89.4% | 93% | (many diffs) |
| 8 | mouse | 55988 | Snx12 | sorting nexin 12 | NM_001110310.1 | 88.2% | 92.4% | (many diffs) |
| 9 | mouse | 55988 | Snx12 | sorting nexin 12 | XM_011247645.2 | 72% | 73.8% | (many diffs) |
| 10 | mouse | 55988 | Snx12 | sorting nexin 12 | XM_011247646.2 | 69.9% | 73.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggacacggca gtagctgata cccggcgcct taactcgaag ccgcaggacc 121 tgaccgacgc ttacgggccg ccaagtaact tcctggagat cgacatcttt aatcctcaga 181 cggtgggcgt gggacgcgcg cgcttcacca cctatgaggt tcgcatgcgg acaaacctac 241 ctatcttcaa gctaaaggag tcctgcgtac ggcggcgcta cagtgacttt gagtggctga 301 aaaatgagct ggagagagat agcaagattg tagtaccacc actgcctggg aaagccttga 361 agcggcagct ccctttccga ggagatgaag ggatctttga ggagtctttc atcgaagaaa 421 ggaggcaggg cctcgagcag tttattaaca aaattgctgg gcacccactg gctcagaatg 481 aacgctgcct acacatgttc ctgcaagagg aggcaattga caggaactac gtcccgggga 541 agagtcttgc tgtgtcttgc ccaggctgga gtgcagtggc atgcccaact ttcttgtaca 601 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 661 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta