Construct: ORF ccsbBroad304_11974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003329.1_s304c1, BRDN0000407534
- Derived from:
- ccsbBroadEn_11974
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WAC (51322)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11974
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51322 | WAC | WW domain containing adapto... | NM_016628.5 | 99.4% | 98.7% | 1917_1918delAA;1941_1942insTGAAGATG |
| 2 | human | 51322 | WAC | WW domain containing adapto... | NM_100264.3 | 92.5% | 91.8% | 0_1ins135;1782_1783delAA;1806_1807insTGAAGATG |
| 3 | human | 51322 | WAC | WW domain containing adapto... | XM_024448036.1 | 92.5% | 91.8% | 0_1ins135;1782_1783delAA;1806_1807insTGAAGATG |
| 4 | human | 51322 | WAC | WW domain containing adapto... | XM_017016315.2 | 92.4% | 91.6% | (many diffs) |
| 5 | human | 51322 | WAC | WW domain containing adapto... | NM_100486.4 | 83.6% | 82.7% | 610_611ins309;1608_1609delAA;1632_1633insTGAAGATG |
| 6 | human | 51322 | WAC | WW domain containing adapto... | XM_017016317.2 | 76.7% | 75.8% | (many diffs) |
| 7 | human | 51322 | WAC | WW domain containing adapto... | XM_017016318.2 | 76.5% | 75.6% | (many diffs) |
| 8 | human | 51322 | WAC | WW domain containing adapto... | XR_001747110.1 | 53.3% | (many diffs) | |
| 9 | human | 51322 | WAC | WW domain containing adapto... | XR_930491.2 | 52.3% | (many diffs) | |
| 10 | mouse | 225131 | Wac | WW domain containing adapto... | NM_153085.4 | 92.2% | 93.8% | (many diffs) |
| 11 | mouse | 225131 | Wac | WW domain containing adapto... | XM_006525803.3 | 91.4% | 92.9% | (many diffs) |
| 12 | mouse | 225131 | Wac | WW domain containing adapto... | XM_006525804.3 | 90.9% | 92.5% | (many diffs) |
| 13 | mouse | 225131 | Wac | WW domain containing adapto... | NM_001282093.1 | 85.6% | 87% | (many diffs) |
| 14 | mouse | 225131 | Wac | WW domain containing adapto... | XM_006525805.2 | 85.6% | 87% | (many diffs) |
| 15 | mouse | 225131 | Wac | WW domain containing adapto... | XM_011246888.2 | 85.6% | 87% | (many diffs) |
| 16 | mouse | 225131 | Wac | WW domain containing adapto... | NM_001146298.2 | 76.8% | 78.2% | (many diffs) |
| 17 | mouse | 225131 | Wac | WW domain containing adapto... | XM_006525806.3 | 76.1% | 77.5% | (many diffs) |
| 18 | mouse | 225131 | Wac | WW domain containing adapto... | XM_006525807.3 | 75.6% | 77% | (many diffs) |
| 19 | mouse | 225131 | Wac | WW domain containing adapto... | XM_017317866.1 | 70.2% | 71.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2013
- ORF length:
- 1947
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt aatgtatgcg aggaaacagc agagactcag tgatggctgt cacgaccgga 121 ggggggactc gcagccttac caggcactta agtattcatc gaagagtcac cccagtagcg 181 gtgatcacag acatgaaaag atgcgagacg ccggagatcc ttcaccacca aataaaatgt 241 tgcggagatc tgatagtcct gaaaacaaat acagtgacag cacaggtcac agtaaggcca 301 aaaatgtgca tactcacaga gttagagaga gggatggtgg gaccagttac tctccacaag 361 aaaattcaca caaccacagt gctcttcata gttcaaattc acattcttct aatccaagca 421 ataacccaag caaaacttca gatgcacctt atgattctgc agatgactgg tctgagcata 481 ttagctcttc tgggaaaaag tactactaca attgtcgaac agaagtttca caatgggaaa 541 aaccaaaaga gtggcttgaa agagaacaga gacaaaaaga agcaaacaag atggcagtca 601 acagcttccc aaaagatagg gattacagaa gagaggtgat gcaagcaaca gccactagtg 661 ggtttgccag tggaatggaa gacaagcatt ccagtgatgc cagtagtttg ctcccacaga 721 atattttgtc tcaaacaagc agacacaatg acagagacta cagactgcca agagcagaga 781 ctcacagtag ttctacgcca gtacagcacc ccatcaaacc agtggttcat ccaactgcta 841 ccccaagcac tgttccttct agtccattta cgctacagtc tgatcaccag ccaaagaaat 901 catttgatgc taatggagca tctactttat caaaactgcc tacacccaca tcttctgtcc 961 ctgcacagaa aacagaaaga aaagaatcta catcaggaga caaacccgta tcacattctt 1021 gcacaactcc ttccacgtct tctgcctctg gactgaaccc cacatctgca cctccaacat 1081 ctgcttcagc ggtccctgtt tctcctgttc cacagtcgcc aatacctccc ttacttcagg 1141 acccaaatct tcttagacaa ttgcttcctg ctttgcaagc cacgctgcag cttaataatt 1201 ctaatgtgga catatctaaa ataaatgaag ttcttacagc agctgtgaca caagcctcac 1261 tgcagtctat aattcataag tttcttactg ctggaccatc tgctttcaac ataacgtctc 1321 tgatttctca agctgctcag ctctctacac aagcccagcc atctaatcag tctccgatgt 1381 ctttaacatc tgatgcgtca tccccaagat catatgtttc tccaagaata agcacacctc 1441 aaactaacac agtccctatc aaacctttga tcagtactcc tcctgtttca tcacagccaa 1501 aggttagtac tccagtagtt aagcaaggac cagtgtcaca gtcagccaca cagcagcctg 1561 taactgctga caagcagcaa ggtcatgaac ctgtctctcc tcgaagtctt cagcgctcaa 1621 gtagccagag aagtccatca cctggtccca atcatacttc taatagtagt aatgcatcaa 1681 atgcaacagt tgtaccacag aattcttctg cccgatccac gtgttcatta acgcctgcac 1741 tagcagcaca cttcagtgaa aatctcataa aacacgttca aggatggcct gcagatcatg 1801 cagagaagca ggcatcaaga ttacgcgaag aagcgcataa catgggaact attcacatgt 1861 ccgaaatttg tactgaatta aaaaatttaa gatctttagt ccgagtatgt gaaattcaag 1921 caactttgcg agagcaaagg atactatttt tgagacaaca aattaaggaa cttgaaaagc 1981 taaaatcaga attccttcat ggtgtgaaga tgtgcccaac tttcttgtac aaagtggttg 2041 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2101 accggtggcg cgttaagtcg acaatcaacc tctggatta