Construct: ORF ccsbBroad304_11993
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004510.1_s304c1, BRDN0000403623
- Derived from:
- ccsbBroadEn_11993
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTF2 (51507)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11993
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51507 | RTF2 | replication termination fac... | NM_001283037.1 | 15.4% | 12.1% | (many diffs) |
| 2 | human | 51507 | RTF2 | replication termination fac... | NM_001283036.1 | 11.7% | 9.1% | (many diffs) |
| 3 | human | 51507 | RTF2 | replication termination fac... | NM_016407.4 | 11.6% | 9.1% | (many diffs) |
| 4 | human | 51507 | RTF2 | replication termination fac... | NM_001283035.1 | 10.2% | 8.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 192
- ORF length:
- 126
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ttgcgacggg ggaacaatcc ccaagaggca tgaactggtg aaggggccga 121 agaaggttga gaaggcaatt attggtgggc tcctggatgg ggcctggtca ccagaaacac 181 caagccaaga ttacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 241 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 301 caatcaacct ctggatta