Transcript: Human NM_001283037.1

Homo sapiens replication termination factor 2 (RTF2), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
RTF2 (51507)
Length:
1617
CDS:
114..806

Additional Resources:

NCBI RefSeq record:
NM_001283037.1
NBCI Gene record:
RTF2 (51507)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001283037.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000365074 GTCGACAAAGATGCTGAATTA pLKO_005 183 CDS 100% 13.200 18.480 N RTF2 n/a
2 TRCN0000142822 CAGCCTTGATTCTAGAGAGAA pLKO.1 751 CDS 100% 4.950 6.930 N RTF2 n/a
3 TRCN0000143283 GCCCAATGGAACTATTGTACT pLKO.1 207 CDS 100% 4.950 6.930 N RTF2 n/a
4 TRCN0000140756 GATGCTGAATTAGTGGCCCAA pLKO.1 192 CDS 100% 2.160 3.024 N RTF2 n/a
5 TRCN0000122229 GCAATAAATCAAGTAGGCCAA pLKO.1 1353 3UTR 100% 2.160 3.024 N RTF2 n/a
6 TRCN0000365073 GTGAACTTGGCAGACTTTATA pLKO_005 265 CDS 100% 15.000 10.500 N RTF2 n/a
7 TRCN0000370062 TGCAGAGTTGGCACATATTAA pLKO_005 1302 3UTR 100% 15.000 10.500 N RTF2 n/a
8 TRCN0000370061 AGGCCTACAAGTCCCTCTTTA pLKO_005 885 3UTR 100% 13.200 9.240 N RTF2 n/a
9 TRCN0000122542 CCAAGTGAAACTGGGCTTTAA pLKO.1 1370 3UTR 100% 13.200 9.240 N RTF2 n/a
10 TRCN0000370018 GAAGGCAGCATCTCACATTAA pLKO_005 341 CDS 100% 13.200 9.240 N RTF2 n/a
11 TRCN0000140232 GCCAAGTGAAACTGGGCTTTA pLKO.1 1369 3UTR 100% 10.800 7.560 N RTF2 n/a
12 TRCN0000143623 GTTGAGAAGGTCGACAAAGAT pLKO.1 174 CDS 100% 5.625 3.938 N RTF2 n/a
13 TRCN0000139552 CCTGTGAACTTGGCAGACTTT pLKO.1 262 CDS 100% 4.950 3.465 N RTF2 n/a
14 TRCN0000140849 CAAGAGGCATGAACTGGTGAA pLKO.1 140 CDS 100% 4.050 2.835 N RTF2 n/a
15 TRCN0000217960 CAAGTGAAACTGGGCTTTAAA pLKO_005 1371 3UTR 100% 15.000 9.000 N RTF2 n/a
16 TRCN0000122230 GTACTCTAAGTCAGGAAATAT pLKO.1 223 CDS 100% 15.000 9.000 N RTF2 n/a
17 TRCN0000142823 CAGCAATGAATGAGAGCTCTT pLKO.1 798 CDS 100% 4.050 2.430 N RTF2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001283037.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08302 pDONR223 100% 75% 66.6% None 511A>G;588_589ins55;690_691ins173 n/a
2 ccsbBroad304_08302 pLX_304 0% 75% 66.6% V5 511A>G;588_589ins55;690_691ins173 n/a
3 TRCN0000472558 TTTCACGACAACCCCGTATTTTAA pLX_317 47.1% 75% 66.6% V5 511A>G;588_589ins55;690_691ins173 n/a
4 ccsbBroadEn_11993 pDONR223 100% 15.4% 12.1% None (many diffs) n/a
5 ccsbBroad304_11993 pLX_304 0% 15.4% 12.1% V5 (many diffs) n/a
6 TRCN0000491291 TGCCCGGATGGCTATCTTCTTGAG pLX_317 100% 15.4% 12.1% V5 (many diffs) n/a
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