Construct: ORF ccsbBroad304_12043

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016761.1_s304c1, BRDN0000399532
Derived from:
ccsbBroadEn_12043
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RETREG1 (54463)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12043
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54463 RETREG1 reticulophagy regulator 1 XM_024446117.1 43.7% 43.7% 1_543del
2 human 54463 RETREG1 reticulophagy regulator 1 XM_024446118.1 43.7% 43.7% 1_543del
3 human 54463 RETREG1 reticulophagy regulator 1 NM_019000.4 39.6% 39.6% 1_645del
4 human 54463 RETREG1 reticulophagy regulator 1 XM_011514055.3 38.7% 38.7% 1_669del
5 human 54463 RETREG1 reticulophagy regulator 1 XM_011514054.2 35.6% 35.6% 1_765del
6 human 54463 RETREG1 reticulophagy regulator 1 NM_001034850.2 28.3% 28.3% 1_1068del
7 human 54463 RETREG1 reticulophagy regulator 1 XM_011514053.3 26.2% 26.2% 1_1188del
8 mouse 66270 Retreg1 reticulophagy regulator 1 NM_001277318.1 37.1% 37.8% (many diffs)
9 mouse 66270 Retreg1 reticulophagy regulator 1 NM_001277317.1 34.5% 35.2% (many diffs)
10 mouse 66270 Retreg1 reticulophagy regulator 1 NM_001277315.1 33.5% 34.2% (many diffs)
11 mouse 66270 Retreg1 reticulophagy regulator 1 NM_025459.3 33.5% 34.2% (many diffs)
12 mouse 66270 Retreg1 reticulophagy regulator 1 XM_006520134.3 33.5% 34.2% (many diffs)
13 mouse 66270 Retreg1 reticulophagy regulator 1 XM_017316720.1 33.5% 34.2% (many diffs)
14 mouse 66270 Retreg1 reticulophagy regulator 1 NM_001277316.1 32.8% 33.5% (many diffs)
15 mouse 66270 Retreg1 reticulophagy regulator 1 NM_001034851.2 24.9% 25.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
489
ORF length:
423
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg aacaaatgat gaagatgaat taagccttgg tttgcccact gagctcaaga
121gaaagaagga acagttggac agtggtcaca gaccaagcaa agagacgcaa tcagcagctg
181gtctcaccct tcctctgaac agtgaccaaa cctttcacct gatgagcaac ctggctgggg
241atgttatcac agctgcagtg actgcagcta tcaaagacca gttagagggt gtgcagcaag
301cactttctca ggctgccccc atcccagaag aggacacaga cactgaagaa ggtgatgact
361ttgaactact tgaccagtca gagctggatc aaattgagag tgaattggga cttacacaag
421accaggaagc agaagcacag caaaataaga agtcttcagg tttcctttca aatctgctgg
481gaggccatta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
541tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
601tcaacctctg gatta

Download FASTA (ORF) (Full)