Construct: ORF ccsbBroad304_12077

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF016216.1_s304c1, BRDN0000402717
• Derived from: ccsbBroadEn_12077
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: RNF111 (54778)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_12077
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	54778	RNF111	ring finger protein 111	NM_001270529.2	13.9%	13.9%	1_2544del
2	human	54778	RNF111	ring finger protein 111	XM_017022343.1	13.9%	13.9%	1_2544del
3	human	54778	RNF111	ring finger protein 111	NM_017610.8	13.8%	13.8%	1_2547del
4	human	54778	RNF111	ring finger protein 111	XM_017022342.1	13.8%	13.8%	1_2547del
5	human	54778	RNF111	ring finger protein 111	XM_017022340.2	13.7%	13.7%	1_2544del;2735_2758del
6	human	54778	RNF111	ring finger protein 111	XM_017022341.1	13.7%	13.7%	1_2544del;2735_2758del
7	human	54778	RNF111	ring finger protein 111	NM_001270530.1	13.7%	13.7%	1_2547del;2738_2761del
8	human	54778	RNF111	ring finger protein 111	XM_017022338.2	13.7%	13.7%	1_2547del;2738_2761del
9	human	54778	RNF111	ring finger protein 111	XM_017022339.2	13.7%	13.7%	1_2571del
10	human	54778	RNF111	ring finger protein 111	NM_001270528.2	13.7%	13.7%	1_2574del
11	human	54778	RNF111	ring finger protein 111	XM_017022337.2	13.6%	13.6%	1_2571del;2762_2785del
12	human	54778	RNF111	ring finger protein 111	NM_001330331.2	13.6%	13.6%	1_2574del;2765_2788del
13	human	54778	RNF111	ring finger protein 111	XM_006720579.3	13.6%	13.6%	1_2574del;2765_2788del
14	human	54778	RNF111	ring finger protein 111	XM_017022336.1	13.5%	13.5%	1_2616del
15	human	54778	RNF111	ring finger protein 111	XM_011521699.2	13.5%	13.5%	1_2631del
16	human	54778	RNF111	ring finger protein 111	XM_006720575.3	13.3%	13.3%	1_2643del;2834_2857del
17	human	54778	RNF111	ring finger protein 111	XM_011521697.3	13.3%	13.3%	1_2655del;2846_2869del
18	human	54778	RNF111	ring finger protein 111	XM_011521696.2	13.2%	13.2%	1_2682del
19	human	54778	RNF111	ring finger protein 111	XM_011521694.2	13.1%	13.1%	1_2682del;2873_2896del
20	mouse	93836	Rnf111	ring finger 111	XM_006511587.3	13.3%	13.8%	(many diffs)
21	mouse	93836	Rnf111	ring finger 111	XM_006511586.2	13.3%	13.8%	(many diffs)
22	mouse	93836	Rnf111	ring finger 111	NM_033604.2	13.2%	13.7%	(many diffs)
23	mouse	93836	Rnf111	ring finger 111	XM_006511582.2	13%	13.6%	(many diffs)
24	mouse	93836	Rnf111	ring finger 111	XM_006511583.3	13%	13.6%	(many diffs)
25	mouse	93836	Rnf111	ring finger 111	XM_006511584.2	13%	13.6%	(many diffs)
26	mouse	93836	Rnf111	ring finger 111	XM_006511581.3	12.1%	12.6%	(many diffs)
27	mouse	93836	Rnf111	ring finger 111	XM_006511580.3	12%	12.5%	(many diffs)
28	mouse	93836	Rnf111	ring finger 111	XM_017313709.1	12%	12.5%	(many diffs)
29	mouse	93836	Rnf111	ring finger 111	XM_006511578.3	12%	12.5%	(many diffs)
30	mouse	93836	Rnf111	ring finger 111	XM_006511577.3	11.9%	12.4%	(many diffs)
31	mouse	93836	Rnf111	ring finger 111	XM_011242841.2	11.9%	12.4%	(many diffs)
32	mouse	93836	Rnf111	ring finger 111	XM_006511576.2	11.9%	12.3%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

477

ORF length:

411

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatggt tcctgatatg gcaggctatc ctcacatccg ttacatttca tcaggattgg
121	atggaacatc attcagaggt cctttcaggg gcaattttga ggaactgatt catttggaag
181	aaagattagg caatgtcaat cgtggagcat cccaggggac aattgaaaga tgtacatatc
241	cacataaata caaaaagagg aaactgcact gcaaacaaga tggggaagaa gggactgagg
301	aagacacaga ggaaaaatgt actatctgtt tgtctatttt agaggaaggt gaagatgtga
361	gacgtcttcc atgtatgcac cttttccacc aagtgtgtgt tgaccaatgg ttgattacca
421	ataagaagtg ccccatatgc agagtggaca ttgaggccca gctgccaagt gaaagttgcc
481	caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
541	ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
601	tta

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