Construct: ORF ccsbBroad304_12085
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014007.1_s304c1, BRDN0000406414
- Derived from:
- ccsbBroadEn_12085
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIT1 (54802)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12085
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | XM_006710706.1 | 99.3% | 99.3% | 279_284delAGTTCT |
| 2 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NM_001312692.1 | 84.1% | 84.1% | 1_183del |
| 3 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | XM_005270954.2 | 83.9% | 83.9% | 1_180del;459_464delAGTTCT |
| 4 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NM_017646.6 | 69.3% | 69.3% | 1_423del;702_707delAGTTCT |
| 5 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NM_001312691.1 | 63.8% | 63.8% | 1_423del;702_707delAGTTCT;928_929ins78 |
| 6 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132408.1 | 51.2% | (many diffs) | |
| 7 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132407.1 | 51.2% | (many diffs) | |
| 8 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132409.1 | 45.9% | (many diffs) | |
| 9 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132402.1 | 45.1% | (many diffs) | |
| 10 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132403.1 | 45.1% | (many diffs) | |
| 11 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132401.1 | 45% | 1_443del;720_723delTTCTinsGC;1418_2155del | |
| 12 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132404.1 | 45% | (many diffs) | |
| 13 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132405.1 | 44.9% | (many diffs) | |
| 14 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132410.1 | 44.9% | (many diffs) | |
| 15 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | XR_946672.2 | 44.1% | (many diffs) | |
| 16 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132415.1 | 43.2% | (many diffs) | |
| 17 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132406.1 | 39.6% | (many diffs) | |
| 18 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132412.1 | 39.5% | (many diffs) | |
| 19 | human | 54802 | TRIT1 | tRNA isopentenyltransferase 1 | NR_132413.1 | 30.3% | (many diffs) | |
| 20 | mouse | 66966 | Trit1 | tRNA isopentenyltransferase 1 | XM_017320359.1 | 64.8% | 61.7% | (many diffs) |
| 21 | mouse | 66966 | Trit1 | tRNA isopentenyltransferase 1 | NM_025873.2 | 60.5% | 61.2% | (many diffs) |
| 22 | mouse | 66966 | Trit1 | tRNA isopentenyltransferase 1 | XR_376339.3 | 35.3% | (many diffs) | |
| 23 | mouse | 66966 | Trit1 | tRNA isopentenyltransferase 1 | XR_376340.3 | 30.3% | (many diffs) | |
| 24 | mouse | 66966 | Trit1 | tRNA isopentenyltransferase 1 | XR_376341.3 | 29.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1038
- ORF length:
- 972
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg cactgagaaa gtgattgacc gaaaagtgga gcttgaaaag gaggatggtc 121 ttgtacttca caaacgccta agccaggtgg acccagaaat ggctgccaag ctgcatccac 181 atgacaaacg caaagtggcc aggagcttgc aagtttttga agaaacagga atctctcata 241 gtgaatttct ccatcgtcaa catacggaag aaggtggtgg tccccttgga ggtcctctga 301 agttctctaa cccttgcatc ctttggcttc atgctgacca ggcagatgag cgcttggata 361 agagggtgga tgacatgctt gctgctgggc tcttggagga actaagagat tttcacagac 421 gctataatca gaagaatgtt tcggaaaata gccaggacta tcaacatggt atcttccaat 481 caattggctt caaggaattt cacgagtacc tgatcactga gggaaaatgc acactggaga 541 ctagtaacca gcttctaaag aaaggtattg aggctctgaa acaagtaact aagagatatg 601 cccggaaaca aaaccgatgg gttaaaaacc gttttttgag cagacctggt cccattgtcc 661 cccctgtcta tggcttagag gtatctgatg tctcgaagtg ggaagagtct gttcttgaac 721 ctgctcttga aatcgtgcaa agtttcatcc agggccacaa gcctacagcc actccaataa 781 agatgccata caatgaagct gagaacaaga gaagttatca cctgtgtgac ctctgtgatc 841 gaatcatcat tggggatcgc gaatgggcag cgcacataaa atccaaatcc cacttgaacc 901 aactgaagaa aagaagaaga ttggactcag atgctgtcaa caccatagaa agtcagagtg 961 tttccccaga ccataacaaa gaacctaaag agaagggatc cccagggcag aatgatcaag 1021 agctgaaatg cagcgtttac ccaactttct tgtacaaagt ggttggtaag cctatcccta 1081 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1141 agtcgacaat caacctctgg atta