Construct: ORF ccsbBroad304_12111
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015033.1_s304c1, BRDN0000402496
- Derived from:
- ccsbBroadEn_12111
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHTF8 (54921)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12111
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113455421 | DERPC | DERPC proline and glycine r... | NM_001002847.3 | 8.3% | 8.3% | 1_1440del |
| 2 | human | 113455421 | DERPC | DERPC proline and glycine r... | NM_001040144.2 | 8.3% | 8.3% | 1_1440del |
| 3 | human | 113455421 | DERPC | DERPC proline and glycine r... | NM_001366603.1 | 8.3% | 8.3% | 1_1440del |
| 4 | human | 113455421 | DERPC | DERPC proline and glycine r... | NM_001366604.1 | 8.3% | 8.3% | 1_1440del |
| 5 | mouse | 214987 | Chtf8 | CTF8, chromosome transmissi... | XM_017312654.1 | 7.5% | 7.5% | (many diffs) |
| 6 | mouse | 214987 | Chtf8 | CTF8, chromosome transmissi... | XM_017312655.1 | 7.5% | 7.5% | (many diffs) |
| 7 | mouse | 214987 | Chtf8 | CTF8, chromosome transmissi... | XM_017312656.1 | 7.5% | 7.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 198
- ORF length:
- 132
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa tggccctgca ggcaagagtt tcgtcccatt tcctagagtg gggagcctcc 121 ctggcacaaa cccagctgct ttccccagac cagggggtcc aatggctgca atgtacccaa 181 atggaatgtt gcccccttac ccaactttct tgtacaaagt ggttggtaag cctatcccta 241 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 301 agtcgacaat caacctctgg atta