Construct: ORF ccsbBroad304_12227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010109.1_s304c1, BRDN0000461085
- Derived from:
- ccsbBroadEn_12227
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MED29 (55588)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12227
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55588 | MED29 | mediator complex subunit 29 | NM_017592.4 | 100% | 100% | |
| 2 | human | 55588 | MED29 | mediator complex subunit 29 | NM_001317770.3 | 80.5% | 63.8% | 359_360ins70;531_588del |
| 3 | human | 55588 | MED29 | mediator complex subunit 29 | NR_133915.3 | 15% | 1_45del;261_262ins59;587_3531del | |
| 4 | mouse | 67224 | Med29 | mediator complex subunit 29 | NM_026042.3 | 84.9% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 669
- ORF length:
- 600
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgcatcc cagcagcaag cttcagcggc ttcctcagct gctggtgtat 121 cgggtcctag ttcggctggc ggcccgggtc cccagcagca gccgcaaccg ccagcacaac 181 tggtgggccc tgcccagagc ggcctcctgc agcaacagca acaggacttc gatcctgtgc 241 agcgttataa gatgctcatc ccgcagctga aggagagtct acagaccttg atgaaggttg 301 cggcccaaaa cttgattcag aacactaaca tcgacaatgg acaaaagagc agtgatggac 361 ccatacagcg ctttgacaag tgcctggaag agttctatgc actctgtgac cagctggagc 421 tgtgcctgcg cctggcgcat gagtgcctgt cacagagttg tgacagtgcc aagcactctc 481 caacgttggt gcccacagcc accaagcccg acgcagtgca gcctgacagc ctcccctacc 541 cacagtacct ggcggtcatc aaagcccaga tttcctgtgc caaggacatt cacaccgccc 601 tgctggactg tgccaacaag gtcacgggca agacacccgc accacctgct ggccctgggg 661 gcactctgtt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 721 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 781 tcaacctctg gatta