Construct: ORF ccsbBroad304_12250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002234.1_s304c1, BRDN0000407274
- Derived from:
- ccsbBroadEn_12250
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIMS2 (55679)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12250
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001256542.1 | 21.6% | 21.6% | 1_444del |
| 2 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452985.1 | 21.6% | 21.6% | 1_444del |
| 3 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452986.1 | 21.6% | 21.6% | 1_444del |
| 4 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_006712627.4 | 18.2% | 18.2% | 1_552del |
| 5 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_017004469.1 | 17.2% | 17.2% | 1_591del |
| 6 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_005263710.2 | 15% | 15% | 1_693del |
| 7 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_011511453.1 | 12.6% | 12.6% | 1_849del |
| 8 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001161404.1 | 12.2% | 12.2% | 1_885del |
| 9 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452983.1 | 12.2% | 12.2% | 1_885del |
| 10 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452984.1 | 12.2% | 12.2% | 1_885del |
| 11 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001161403.2 | 12% | 12% | 1_900del |
| 12 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001136037.3 | 11.2% | 11.2% | 1_966del |
| 13 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_017980.4 | 11.2% | 11.2% | 1_972del |
| 14 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XR_922961.2 | 8.5% | 1_1064del;1165_1166ins22 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 189
- ORF length:
- 123
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcccgtgtgt aagaggtgct acgagaagtt cccgctggag ctgaagaagc 121 ggctgaagaa gctgtcggag ctgacctccc gcaaggccca gcccaaggcc acagacctca 181 actctgcctg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 241 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 301 tcaacctctg gatta