Construct: ORF ccsbBroad304_12272

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014978.1_s304c1, BRDN0000409326
Derived from:
ccsbBroadEn_12272
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MBD5 (55777)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12272
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55777 MBD5 methyl-CpG binding domain p... NM_018328.4 14.9% 14.7% (many diffs)
2 human 55777 MBD5 methyl-CpG binding domain p... XM_024452990.1 14.8% 14.5% (many diffs)
3 human 55777 MBD5 methyl-CpG binding domain p... XR_002959319.1 13.7% (many diffs)
4 human 55777 MBD5 methyl-CpG binding domain p... XM_024452989.1 13.1% 12.9% (many diffs)
5 human 55777 MBD5 methyl-CpG binding domain p... XM_024452988.1 13% 12.8% (many diffs)
6 human 55777 MBD5 methyl-CpG binding domain p... XM_024452987.1 12.9% 12.7% (many diffs)
7 human 55777 MBD5 methyl-CpG binding domain p... XM_011511470.2 12.8% 12.6% (many diffs)
8 human 55777 MBD5 methyl-CpG binding domain p... XM_011511472.2 12.8% 12.6% (many diffs)
9 human 55777 MBD5 methyl-CpG binding domain p... XR_002959318.1 12.2% (many diffs)
10 mouse 109241 Mbd5 methyl-CpG binding domain p... XM_017314916.1 16.3% 16% (many diffs)
11 mouse 109241 Mbd5 methyl-CpG binding domain p... NM_001290656.1 14.4% 14.2% (many diffs)
12 mouse 109241 Mbd5 methyl-CpG binding domain p... XM_017314915.1 14.4% 14.2% (many diffs)
13 mouse 109241 Mbd5 methyl-CpG binding domain p... XM_006497604.2 13.4% 13.2% (many diffs)
14 mouse 109241 Mbd5 methyl-CpG binding domain p... XM_017314914.1 12.9% 12.7% (many diffs)
15 mouse 109241 Mbd5 methyl-CpG binding domain p... NM_029924.2 12.5% 12.3% (many diffs)
16 mouse 109241 Mbd5 methyl-CpG binding domain p... XM_017314909.1 12.5% 12.3% (many diffs)
17 mouse 109241 Mbd5 methyl-CpG binding domain p... XM_017314910.1 12.5% 12.3% (many diffs)
18 mouse 109241 Mbd5 methyl-CpG binding domain p... XM_017314911.1 12.5% 12.3% (many diffs)
19 mouse 109241 Mbd5 methyl-CpG binding domain p... XM_017314912.1 12.5% 12.3% (many diffs)
20 mouse 109241 Mbd5 methyl-CpG binding domain p... XR_001780404.1 6.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
753
ORF length:
687
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt ccctcctact gccaacatgc ttctcccaac aggtgaaggg caaagtggtc
121gagcagcact aagagataag ctgatgtctc agcaaaaaga cgcattgcgg aaaagaaaac
181aaccacctac gacagtgttg agtttgctca gacagtctca aatggatagt tctgcagttc
241ctaaacctgg acctgacttg ctaaggaagc agggtcaggg ttcatttccc atcagttcaa
301tgtctcagtt actacagtct atgagttgtc aaagctctca cttgagtagc aatagtaccc
361cgggttgtgg ggcctcaaat actgctttgc cttgctctgc taaccagctg cattttacag
421atcccagtat gaactctagt gttcttcaga acataccttt aagaggggaa gccgtgcact
481gccacaatgc aaacactaac tttgttcaca gtaacagtcc agtccccaac caccatcttg
541caggtttaat aaatcagatt caggctagcg ggaactgtgg gatgctcagt cagtcgggca
601tggctttagg aaattcctta catcccaatc cacctcagtc aagaatttca acgtcctcca
661ctccagtgat accaaacagc attgttagca gctataatca aacaagttct gaagcaggta
721tggttttatt agaaaaaagt acccaaaggt actacccaac tttcttgtac aaagtggttg
781gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
841accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)